We narrowed to 961 results for: REP

1. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ... efficiently. For select viral vectors in the repository, you can instead have Addgene make the virus ...

2. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   ...important resource that Addgene provides is an open repository of plasmids and other necessary information that...

3. 15 Years of Addgene: The Top 15 Plasmids

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   ...plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and...

4. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...

5. A Deep Dive into BioBuilder

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   ... The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged...

6. Improved Plasmid Maps Powered by SnapGene

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   ...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

7. Luminescent Imaging with Nano-lanterns

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   ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

8. How to Keep a Lab Notebook for Bioinformatic Analyses

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   ... for each analysis or task will keep you from repeating your work or making similar mistakes in the future...

9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...factors. The second vector is a homology directed repair donor plasmid with homology arms and a selection...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

11. 3 Challenges in Plant Synthetic Biology

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    ...comprehensive plant cloning kits available in the repository, MoClo and GreenGate, and we are excited to see...

12. Antibodies 101: Conjugation

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    ... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...

13. dTAG - You're it!

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    ... 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in human cells...

14. Transferable Skills Guide: Public Speaking

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    ... the question, you need only to take that step. Repeat that as often as needed until you get to the end...

15. Transferable Skills Guide: Cross-team Communication

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    ...miscommunication and b) find practical solutions.  My PhD prepared me remarkably well for this task. Good scientists...

16. Antibodies 101: Selecting the Right Antibody

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    ...different samples and experimental conditions. Be prepared to troubleshoot and be sure to include proper ...

17. Celebrating 15 Years of Scientific Sharing

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    ...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

18. Developing Lab Management Software for Biology

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    ...Other steps fail “soft” they simply need to be repeated. We also now have a good interface to begin tracking...

19. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post

    ...Homolog Cpf1 Read How Non-Homologous End Joining Repairs Double Strand Breaks Get Tips on Designing Your...

20. Plasmids 101: Modular Cloning

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    ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...