We narrowed to 957 results for: REP

1. Viral Vectors 101: The AAV Retrograde Serotype

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   ..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

2. Antibodies 101: Flow Cytometry Controls

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   ...interest. Using isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample...

3. A Deep Dive into BioBuilder

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   ... The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged...

4. How to Keep a Lab Notebook for Bioinformatic Analyses

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   ... for each analysis or task will keep you from repeating your work or making similar mistakes in the future...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...factors. The second vector is a homology directed repair donor plasmid with homology arms and a selection...

6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

7. 3 Challenges in Plant Synthetic Biology

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   ...comprehensive plant cloning kits available in the repository, MoClo and GreenGate, and we are excited to see...

8. dTAG - You're it!

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   ... 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in human cells...

9. Celebrating 15 Years of Scientific Sharing

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   ...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

10. Antibodies 101: Selecting the Right Antibody

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    ...different samples and experimental conditions. Be prepared to troubleshoot and be sure to include proper ...

11. Transferable Skills Guide: Public Speaking

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    ... the question, you need only to take that step. Repeat that as often as needed until you get to the end...

12. Antibodies 101: Conjugation

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    ... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...

13. Transferable Skills Guide: Cross-team Communication

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    ...miscommunication and b) find practical solutions.  My PhD prepared me remarkably well for this task. Good scientists...

14. Developing Lab Management Software for Biology

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    ...Other steps fail “soft” they simply need to be repeated. We also now have a good interface to begin tracking...

15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...Homolog Cpf1 Read How Non-Homologous End Joining Repairs Double Strand Breaks Get Tips on Designing Your...

16. Engaging with science and society at pgEd

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    ...running these events will be important both in preparing you for future work in these areas, and in helping...

17. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...riboprobes are created, the WISH protocol involves preparation of the tissue sample, several washes, and temperature...

18. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...and labeled protein interactors purified via streptavidin pulldown. Following trypsin digest, PPIs are...

19. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...

20. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...to be creative. After all, your poster is a representation of your work and a reflection of you, so make...