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...of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI...AAV1 AAV2 AAV5 AAV6 AAV8 AAV11 AAVrg Want more information before choosing a serotype? Learn more about ... can do this whenever you are ready. Payment information must be added before we can begin processing ...this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes to make...Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping time will...whether the item can be packaged. Addgene does not perform functional or in vivo testing. We recommend that...Addgene’s Help Center, a searchable database of information to help you every step of the way, including ...

... is stable at 4°C for up to one year. The powder form of polybrene is stable at 4°C for several years....provided for your convenience. See “warranty information” in appendix. Table of Contents A. pLKO.1-TRC...Digesting pLKO.1 TRC-Cloning Vector C.4 Ligating and transforming into bacteria D. Screening for Inserts D.1 Recommended... TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 ...pCMV-VSVG from Robert Weinberg’s lab. For more information, visit Addgene’s Mammalian RNAi Tools page. Several...Measure the DNA concentration. C.4 Ligating and Transforming into Bacteria Use your ligation method of choice...final volume Incubate at 16°C for 4-20 hours. Transform 2 μL of ligation mix into 25 μL competent DH5 ...

...methods, and quality control are described below. Information for a specific antibody can be found on the antibody's...reviewing the specific antibody webpage for further information. Production For recombinant antibodies (rAb) ...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...recommended applications. The specific QC experiments performed varies for each antibody catalog item. Whenever...Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or the.... To learn which specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry...

...literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information (Link...which they were created. Find their contact information by following the link to their laboratory website...Link opens in a new window) Wendy Gold For more information on generating iPSCs using plasmids, please see...MECP2 - MECP2 curated protein and functional information Mouse and Cell Line Repositories (Link opens ...Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH image data...

...MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available to download...window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to assay...techniques. Using a single protein with many available isoforms and mutants provides a shared experience for the...mentor, and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student...

...into an expression vector of their choice and transforming into E. coli bacteria. This can help reduce ...genetic engineering and directed evolution. More information about the collection can be obtained at the Open...an expression vector of their choice and then transform into E. coli for expression and purification (... in this collection is ampicillin. Find more information on the enzymes and protocols for expression in...Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 PGD T4 PGD...

...and resources are up-to-date with reagents and information you need to use CRISPR in your own lab. Check...Resources CRISPR Guide : Essential background information on CRISPR and the basics for planning your first...to reverse transcriptase install new genetic information into a specific DNA site. Base Edit Base editors...editors precisely edit DNA without promoting DSB formation. Delete Cascade-Cas3 generates large genomic deletions...

... Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic protein ...labeling in a sample. Please see the note below for information on substrates and synthases needed for use of...Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP tag FAQs (PDF... Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic...

...experiments to measure protein interactions or conformation changes....can use FRET to report on protein binding or conformational changes. Organic dyes, fluorescent proteins... intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and...and explore the tables below for plasmids and information to help plan your next FRET experiment. Looking...

...viral vector production at Addgene, ordering information, and more. Additional Resources Addgene's Data...from the research community demonstrating AAV performance under various experimental conditions. Protocols...institutional biosafety office and your lab, information on the biosafety of viral vectors, and general...

...CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of ...al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a single...Maeder et al., Nat Methods 2013 ) More detailed information about REAL, REAL-Fast and FLASH, including detailed...

...been cloned, expressed and validated in recombinant form. The recombinant monoclonal antibodies or R-mAbs...IgG2a 149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a 149453 Anti-Kv4.2...IgG2a 188208 Anti-FGF13/FHF2, B isoform [N225A/10R] FGF13/FHF2, B isoform Human Mouse IgG2a 188209 Anti-...190541 FGF13/FHF2, B isoform scFv [N225A/10] N225A/10 scFv FGF13/FHF2, B isoform Human Mouse 190542 Pan-Shank...

... Browse constructs currently available in viral format....AAV5, AAV6, AAV8, AAV11, or AAVrg). For more information, visit our Viral Vector Packaging Service overview...

...the attachment site of the phage 186. Detailed information about the repression system can be found in this...PMID: 29519933 . bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be...

...guarantee the function of any viral vector. Addgene performs QC measures which verify the identity of the viral...shipped, Addgene can provide the QC validation data performed on the material received. Shipping While Addgene...

... in the collection have been developed using a platform (Link opens in a new window) based on yeast display...tools to investigate neurotransmission, synapse formation, neural connectivity, plasticity and neurological...

...vectors are popular tools for delivering genetic information to cells. These vectors rely on production plasmids... consist of a plasmid containing the genetic information to be delivered (transfer or shuttle plasmid)...

...may be available, click on the links for more information. Plasmids are available for all the items listed...) what you would like to see available in viral format. Please note this does not guarantee viral service...

...proteins or viral proteins (VP1, VP2 and VP3) that form the icosahedral capsid of the virus. The ITR sequences...Sf9 cells , have also been developed. The newly formed viral particles produced in HEK293T cells are then...molecule that spontaneously reanneals upon infection, forming a double-stranded DNA (dsDNA) template. This eliminates... When these viral vectors co-infect a cell, they form concatemers and splice together, reconstructing ...capsid proteins VP1, VP2, and VP3, which together form the outer capsid shell that protects the viral genome...Recombinant adeno-associated viral (rAAV) vector Modified form of AAV used to deliver genetic material into cells...of experiments being performed. Your biosafety office can provide more information on your institution'...

... Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation should be...of 5 mL (3 mL SOC + 2 mL transformed Endura from two separate transformations). Pro-Tip Do not pipette...Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli ...assumes familiarity with standard bacterial transformation and basic knowledge of the library being amplified...amplified. Please see our protocol on Bacterial Transformation . The generalized pooled library amplification... be performed at the end of the day to ensure that growth times are limited. Day 2: Harvest cells and ...using a new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes...