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TypeCollection...and resources are up-to-date with reagents and information you need to use CRISPR in your own lab. Check...Resources CRISPR Guide : Essential background information on CRISPR and the basics for planning your first...to reverse transcriptase install new genetic information into a specific DNA site. Base Edit Base editors...editors precisely edit DNA without promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic...
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New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection... Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic protein ...labeling in a sample. Please see the note below for information on substrates and synthases needed for use of...Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP tag FAQs (PDF... Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic... -
Viral Vectors
TypeCollection...genetic information, viruses are a powerful tool for the delivery of this genetic information to cells.... Viruses facilitate the delivery of genetic information to hard-to-transfect mammalian cells. In addition...of different types of viruses. More detailed information can be found in the guides for each virus type... -
TALEN Engineering
TypeCollection...CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of ...al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a single...Maeder et al., Nat Methods 2013 ) More detailed information about REAL, REAL-Fast and FLASH, including detailed... -
Trimmer Lab NeuroMab Collection
TypeCollection...been cloned, expressed and validated in recombinant form. The recombinant monoclonal antibodies or R-mAbs...IgG2a 149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a 149453 Anti-Kv4.2...IgG2a 188208 Anti-FGF13/FHF2, B isoform [N225A/10R] FGF13/FHF2, B isoform Human Mouse IgG2a 188209 Anti-...190541 FGF13/FHF2, B isoform scFv [N225A/10] N225A/10 scFv FGF13/FHF2, B isoform Human Mouse 190542 Pan-Shank... -
Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids
TypeCollection...programmable endonucleases that can be used to perform targeted genome editing. RGNs consist of two components...needed to construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems can also be... -
AAV Viral Preps
TypeCollection... Browse constructs currently available in viral format....(AAV1, AAV2, AAV5, AAV8, or AAVrg). For more information, visit our Viral Vector Packaging Service overview... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...the attachment site of the phage 186. Detailed information about the repression system can be found in this...PMID: 29519933 . bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be... -
Addgene Packaged on Request: Scope of Service
TypeCollection...guarantee the function of any viral vector. Addgene performs QC measures which verify the identity of the viral...shipped, Addgene can provide the QC validation data performed on the material received. Shipping While Addgene... -
CRISPR Pooled gRNA Libraries
TypeCollection...includes information about the type of genetic modification and target species. Additional information about... -
Penn Vector Core Partnership with Addgene
TypeCollection...may be available, click on the links for more information. Plasmids are available for all the items listed...) what you would like to see available in viral format. Please note this does not guarantee viral service... -
Adeno-associated virus (AAV) Guide
TypeGuide...proteins or viral proteins (VP1, VP2 and VP3) that form the icosahedral capsid of the virus. The ITR sequences...Sf9 cells , have also been developed. The newly formed viral particles produced in HEK293T cells are then...molecule that spontaneously reanneals upon infection, forming a double-stranded DNA (dsDNA) template. This eliminates... When these viral vectors co-infect a cell, they form concatemers and splice together, reconstructing ...capsid proteins VP1, VP2, and VP3, which together form the outer capsid shell that protects the viral genome...Recombinant adeno-associated viral (rAAV) vector Modified form of AAV used to deliver genetic material into cells...of experiments being performed. Your biosafety office can provide more information on your institution'... -
KLF Research Plasmids
TypeCollection...addgene.org to get started. Genes KLF gene pages with information on available plasmids, references, and links.... -
Individual REAL TALE Repeat Plasmids
TypeCollection...four TALEN expression vectors. More detailed information about REAL, including detailed and up-to-date... -
Ginkgo Bioworks COVID-19 Collection
TypeCollection...academic and industrial organizations. For more information on ordering from a for-profit entity, please ... -
Cancer Research Plasmids and Resources
TypeCollection...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of... -
Fluorescent Protein Guide
TypeCollection...standards to study protein-protein interactions or conformational changes within a protein. Biosensors Monitor... -
Structural Genomics Consortium Plasmids
TypeCollection...process online. Click here for additional ordering information.... -
Institute for Protein Innovation
TypeCollection... in the collection have been developed using a platform (Link opens in a new window) based on yeast display... -
Brzezinski Lab CRISPR Collection
TypeCollection...all pX458 family plasmids. Additional Cloning Information Cloning Protocols Single guide cloning: Zhang...