Plasmids 101: Optimizing Plasmid Yields
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xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...about Homology Directed Repair Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...Addgene. The CRISPR Journal, 6(6), 493–501. https://doi.org/10.1089/crispr.2023.0059. More resources on the... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...doi: 10.1038/s41598-019-52370-8 Resources on Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection ... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...Plasmids 101: Mammalian Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...: A Practical Guide Additional Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Empty ... -
Academic vs. Industry Postdocs
TypeBlog Post...Kamen's 8 tactics for choosing a lab Resources on Addgene.org Find open positions at Addgene Check out our... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene produces AAV and lentivirus... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...other plasmid cloning techniques Resources on Addgene.org Find basic molecular biology protocols Find ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...Download the Plasmids 101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...provide an on-topic answer by creating time to organize your thoughts. It’s likely you won’t know every... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...professional network Volunteer at a professional organization to expand your skill set Resources on the Addgene... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...libraries Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference plasmids Find... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...gRNAs Find other CRISPR software Resources on Addgene.org Find CRISPR plasmids and resources Download ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...User (by a 1st Time CRISPR User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems... -
Genome-wide Screening Using CRISPR
TypeBlog Post... Protocols in Molecular Biology 115: . https://doi.org/10.1002/cpmb.8 Additional Resources on the Addgene... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...Methods Validate Your Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check... -
Lentiviral Vector Uses and Overview
TypeBlog Post...Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus...