Plasmids 101: Knockout/Knock-In Plasmids
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... Used to Turn Genes On and Off
Resources on Addgene.org
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Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...Lab E. coli Strains Additional Resources on Addgene.org Find Info on How to Quantify Your DNA Learn ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...User (by a 1st Time CRISPR User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems... -
Genome-wide Screening Using CRISPR
TypeBlog Post... Protocols in Molecular Biology 115: . https://doi.org/10.1002/cpmb.8 Additional Resources on the Addgene... -
Simplify Cloning with in vivo Assembly
TypeBlog Post... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here Find... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post... protein expression Additional Resources on Addgene.org Find more nanobody expression plasmids here ... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...science communication blog posts Resources on Addgene.org Check out our comprehensive CRISPR guide Find... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... -
Great Results Start with Great Standard Curves
TypeBlog Post... A Control for All Seasons More resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post... about CRISPR DNA Base Editing Resources on Addgene.org Browse All CRISPR Plasmids Check out our CRISPR... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...plasmid sharing during COVID-19 Resources on Addgene.org Find plasmids from David Liu's lab Visit our... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post... CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...of our viral vectors blog posts Resources on Addgene.org Learn our protocol for AAV titration by qPCR... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...Methods Validate Your Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check... -
Lentiviral Vector Uses and Overview
TypeBlog Post...Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus... -
Plasmids 101: Colony PCR
TypeBlog Post...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...Contribute to the Addgene Blog Resources on Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...just starting a lab, or are looking for a way to organize samples within your lab, we highly recommend using...