Skip to main content

We narrowed to 1,154 results for: Gene

Showing: 901 - 920 of 1154 results
  1. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection... it also has several drawbacks. Direct ELISAs generally have higher levels of background staining. Antigen-containing...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting...
  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about...tests must be met. In the Department of Labor’s general guide to the effect on higher education, you can...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...
  3. Zhang Lab CRISPR Page

    Type
    Collection
    ... information, please visit the Addgene SAM library page . Targeted gene activation using SAM There are...available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed...contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...human codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid... and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV...-Cre recombinase and sgRNAs targeting the mouse genes: Kras , p53 , and Lkb1 . This plasmid also contains...
  4. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ... resources at Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook...sure where to start or what your options are? Addgene's latest animation is here to help! "Antibodies...
  5. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to...and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters, Flannel Fridays...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...
  6. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... are essential for processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction...transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...
  7. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Check out additional microbiology resources Stem cell models for...additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...
  8. Cancer Research Plasmids and Resources

    Type
    Collection
    ... RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled... click on a component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by ...of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor and tumor...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer...
  9. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert... Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
  10. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...particularly genetics research, for decades. Its short life cycle, reasonably close genetic homology to...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...
  11. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...
  12. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...We’re big fans of all our depositors here at Addgene — your contributions make the repository what it... designing a novel topology, then iteratively generated 3D models and amino acid sequences that got closer...Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution Ras...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...
  13. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...signs of holiday spirit still linger on around the Addgene office as we peruse the #DeckTheLab submissions...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating...
  14. Retrovirus Plasmids

    Type
    Collection
    ...plasmid — contains packaging genes Envelope plasmid — contains packaging gene env This collection highlights...Transfer Plasmids Want a retroviral plasmid with your gene of interest? Search our database for retroviral ...Puro IRES GFP empty plasmid) MSCV For cloning and gene expression. Select with puromycin or screen for ... the transgene fused to eGFP. Hans Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...can take advantage of this to stably integrate transgenes of interest into a variety of cell types. Wild-type...plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging...
  15. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
  16. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...
  17. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn... I heard an impressive talk about the use of optogenetics tools to inhibit epileptic seizures in a mouse...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...
  18. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...custom KTR design, plasmids are available from Addgene! References Kudo, T., Jeknić, S., Macklin, D.N...KTR plasmids here! Additional Resources on the Addgene Blog Learn about photoswitchable kinases Learn...protein phosphorylation Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors...
  19. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...fluorescent reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...
  20. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...
Showing: 901 - 920 of 1154 results