We narrowed to 936 results for: Kin

1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   ...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...

3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...were the only realistic options for scientists working in regenerative medicine, but the longing for a...

4. Plasmids 101: Protein Expression

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   ...antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems Yeast are a great ...

5. Plasmids 101: Degron Tags

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   ...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...

6. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...

7. Anatomy of a Plasmid Page at Addgene

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   ...found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help...

8. Great Results Start with Great Standard Curves

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   ...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

9. An Introduction to Adenovirus

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   ...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...

10. Tips for arabidopsis transformation

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    ...phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself...

11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...

13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...

14. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...been limited, in part, due to the necessity for working with this virus under the highest biosafety level...

15. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    ... version of TnpB is just 408 amino acids long — making it one of the most compact genome editors to date...

16. QC Sequencing Technologies at Addgene

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    ...choose the best method based on the plasmid. By working with the Data team, the QC team has also improved...

17. Hot Plasmids: Fall 2024

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    ...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...

19. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...

20. New Tools Enable CRISPRa for Neuroscience Applications

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    ...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...