We narrowed to 957 results for: REP

1. CRISPR in the Clinic

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   Blog Post

   ...659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell ...

2. Plasmids 101: Modular Cloning

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   Blog Post

   ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...

3. Progress Towards a PAM-Free CRISPR

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   Blog Post

   ...SpCas9, is NGG. Assuming all nucleotides are represented equally, that’s only a 1/16 chance of having ...

4. Early Career Researcher Toolbox: Finding Relevant Papers

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   Blog Post

   ...PubMed search option in early 2020 and eventually replace the existing version of PubMed.  Key features This...

5. 6 Tips for Grant Writing

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   Blog Post

   ...mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...

6. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   ...your protocols, designed your experiments, and prepared your reagents. You’re going to engineer the best...

7. Plasmids 101: Protein Expression

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   Blog Post

   ... Learn about inducible promoters Learn about repressible promoters Check out protein tags Resources on...

8. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody...

9. Transferable Skills: Negotiation

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   ...process felt unnatural and intimidating. I felt unprepared. Mainly, I thought of negotiation as something...

10. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...in your experiment. Figure 2: Visual representation of common AAV serotypes used for different ...

11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    ...improved expression in living cells. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-48604...

12. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    Blog Post

    ...transformants onto the selection plate. Figure 2 shows representative transformation plates obtained from 1-, 12-...

13. Experimenting with New Careers while in Grad School

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    Blog Post

    ..., though often called “nontraditional,” helped prepare me to start CINSO. Here, I share some of the tips...

14. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...Community Learn the Benefits of Depositing Browse the Repository  ...

15. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz (...

16. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

    ... genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration...

17. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...get maximum satisfaction out your work, and be prepared for new and exciting opportunities. Here are some...

18. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post

    ... others were curious about the agricultural repercussions. Was every student riveted? Absolutely not. ...

19. Getting to Know Expansion Microscopy

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    Blog Post

    ...ensure the expanded images are still a good representation of reality. Third, expansion results in dimmer...

20. Tips for arabidopsis transformation

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    Blog Post

    ...RFP in the seed (Emami, Yee and Dinneny, 2013). Preparing plants for transformation Whatever plants you ...