We narrowed to 961 results for: REP

1. Progress Towards a PAM-Free CRISPR

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   Blog Post

   ...SpCas9, is NGG. Assuming all nucleotides are represented equally, that’s only a 1/16 chance of having ...

2. CRISPR in the Clinic

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   Blog Post

   ...659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell ...

3. Science communication: 9 Strategies to Get Your Foot in the Door

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   Blog Post

   ...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...

4. Engaging with science and society at pgEd

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   Blog Post

   ...running these events will be important both in preparing you for future work in these areas, and in helping...

5. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...riboprobes are created, the WISH protocol involves preparation of the tissue sample, several washes, and temperature...

6. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   Blog Post

   ...and labeled protein interactors purified via streptavidin pulldown. Following trypsin digest, PPIs are...

7. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   Blog Post

   ...to be creative. After all, your poster is a representation of your work and a reflection of you, so make...

8. Early Career Researcher Toolbox: Finding Relevant Papers

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   Blog Post

   ...PubMed search option in early 2020 and eventually replace the existing version of PubMed.  Key features This...

9. 6 Tips for Grant Writing

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   Blog Post

   ...mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...

10. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...in your experiment. Figure 2: Visual representation of common AAV serotypes used for different ...

11. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    Blog Post

    ...transformants onto the selection plate. Figure 2 shows representative transformation plates obtained from 1-, 12-...

12. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...your protocols, designed your experiments, and prepared your reagents. You’re going to engineer the best...

13. Plasmids 101: Protein Expression

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    Blog Post

    ... Learn about inducible promoters Learn about repressible promoters Check out protein tags Resources on...

14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody...

15. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...improved expression in living cells. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-48604...

16. Transferable Skills: Negotiation

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    Blog Post

    ...process felt unnatural and intimidating. I felt unprepared. Mainly, I thought of negotiation as something...

17. Experimenting with New Careers while in Grad School

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    Blog Post

    ..., though often called “nontraditional,” helped prepare me to start CINSO. Here, I share some of the tips...

18. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...Community Learn the Benefits of Depositing Browse the Repository  ...

19. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz (...

20. Getting to Know Expansion Microscopy

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    Blog Post

    ...ensure the expanded images are still a good representation of reality. Third, expansion results in dimmer...