...be targeted with optical stimuli. The restricted light exposure to a specific area in combination with ...

... Since the signal level provided by SunTag is so high, cells may be imaged under lower illumination settings...

...consider. Antibiotic selection Selection is straightforward in cases where the inserted DNA includes an...

...pinpoint exactly when I realized I had made the right decision to choose Addgene for my co-op, but I know...

...Antibody dependent cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC,...

...recommended names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

...’s not our only job - we strive to also give you high-quality educational resources to help you do better...

...document may sound like something out of your nightmares, shared ownership of materials produced from ...

...troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a question about a...

...productivity by providing world-wide access to curated, high-quality data and reagents Addgene makes it easy ...

...faculty and introducing the kits to clients who might initially be doubtful of online science lab classes...

...an opportune time or go all out with constitutive high level expression? Finally, you can add bells and...

...container Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...each well of the 6-well dish. Incubate the cells overnight. If using freshly collected virus, filter through...If you anticipate that your viral stock will be higher titer, consider additional dilutions. Dilution ...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields are...

... the same protein, their gene sequence may have slight or large variations depending on how closely related...

...-enveloped virus (left) and an enveloped virus (right). Created using biorender.com   If you’re new to...

...fertilizer, seeds and a growth space with fluorescent lights. Other experiments require only seeds, petri dishes...

...biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions...

...elements Coding sequence features are relatively straightforward to define, but for control elements such as...

...of troubleshooting failed experiments. Pick the right strain While common E. coli strains like DH5-alpha...