CRISPR 101: Making a Knock-In Cell Line
Type
Blog Post
... CRISPR Editing in Human Cells
Resources on Addgene.org
Browse All CRISPR Plasmids
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Simplify Cloning with in vivo Assembly
TypeBlog Post... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here Find... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post... protein expression Additional Resources on Addgene.org Find more nanobody expression plasmids here ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...Methods Validate Your Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check... -
Lentiviral Vector Uses and Overview
TypeBlog Post...Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus... -
Plasmids 101: Colony PCR
TypeBlog Post...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...Contribute to the Addgene Blog Resources on Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...just starting a lab, or are looking for a way to organize samples within your lab, we highly recommend using... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...directly from A. Victoria and expressed in any organism while still maintaining fluorescence. The protein... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...engine update Find all Addgene news Resources on Addgene.org Check out our job postings Learn more about ... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...Beginner's Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...for gene expression in mammals. Resources on Addgene.org Search for AAV vectors to use with your research... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...sheet Download our CRISPR eBook Resources on Addgene.org Check out our comprehensive CRISPR guide Find... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...end-point data, like our ongoing website survey on addgene.org. Currently, we’re evaluating our depositor process... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant... -
How to Be an Excellent Trainee
TypeBlog Post... to remember in the moment….but you’ll probably forget and end up asking your mentor later if you don’... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature 553, 86–90 (2018... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...drosophila, worms and mammals (Browse plasmids by model organism, here). More information on the various delivery...