We narrowed to 958 results for: Tes

1. Hot Plasmids and Viral Preps - January 2021

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   ...well-characterized Cas enzymes, which rely on multiple active sites. In addition, Casɸ has a minimal PAM requirement...

2. Gaining Leadership Skills Volunteering at a Professional Organization

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   ...them “Leadership and Management skills”. The NPA states that Postdoctoral scholars should have the skills...

3. Academic vs. Industry Postdocs

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   ... companies, such as Novartis, have specific web sites for postdoc listings, so the method of posting varies...

4. Plasmids 101: Protein Expression

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   ...Production of recombinant proteins in plant root exudates Resources on the Addgene Blog Learn about inducible...

5. Degrading DNA with Cascade-Cas3

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   ...unwinding of dsDNA occur simultaneously, which creates a loop in the target strand that is cut occasionally...

6. Production of Virus in Insect Versus Mammalian Cells

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   ...to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in...

7. Viral Vectors 101: Producing Your rAAV

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   ..., E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally...

8. Antibodies 101: Flow Compensation

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   ...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

9. Twenty Years of Addgene Sharing: CRISPR

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   ... and the longevity of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is...

10. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses. In: Methods...

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ... systems. Some resources will direct you to the latest information about gene function, while others offer...

12. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...localization and transport between healthy and diseased states can also provide interesting insights into disease...

13. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's...

14. Anatomy of a Plasmid Page at Addgene

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    ...is an indication of popularity. A yellow flame indicates that a plasmid has been ordered more than 20 times...

15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...effects in human genomes (Doench et al., 2016). To test the library, they conducted genome-wide negative...

16. A Guide to Starting Your Own Journal Club

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    ...scheduling conflicts. This helped us find a time that accommodates more people. The feedback could be solicited...

17. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...about your communication style, your potential teammates want to be assured that you will contribute openness...

18. Lentiviral Vector Uses and Overview

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    ...transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral...

19. CRISPR Antimicrobials

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    ...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...

20. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...early research career. I've found that research institutes in different places have very different cultures...