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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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  3. Transferable Skills: Negotiation

    Type
    Blog Post
    ...specific details about the individuals, each example provides a scenario where both people get something valuable...

  6. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...

  9. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...plasmid of interest. For this example, we will describe how to copy a cDNA from one vector into a new ...(YGOI) for ligation into a recipient plasmid. Designing Primers for PCR Based Cloning The basic PCR primers...that: Do not cut within your insert. Are in the desired location in your recipient plasmid (usually in ...examine the DNA sequence that we want to amplify and design primers that will bind to and replicate it. The...ends of the ORF and how these are used for primer design: Because we are cloning an ORF, we want to clone...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement...

  10. Western Blot

    Type
    Protocol
    ...This protocol describes the basic steps for lysing cells, determining total protein concentration in ...specific to the protein of interest. This protocol describes the basic steps for lysing cells, determining ...continue running the gel until you have obtained the desired separation. Gently open the gel with the spatula...Device. Close the lid of the device. Select the desired method and make sure the parameters are correct... incubation Dilute the primary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...peroxidase-conjugated secondary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...

  15. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...Analysis of a Plasmid You may also like... How to Design a Primer Diagnostic Restriction Digest Introduction...If you require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's...'s sequencing results as a reference for primer design. A good sequencing reaction will produce between...

  16. Water Bath Protocol

    Type
    Protocol
    ... of bacteria or fungi. There are disinfectants designed to be used in water baths with instructions on...bath on and set the appropriate temperature as described in your protocol. How to set the temperature will...Set up your water bath so that it will reach the desired temperature by the time you need it for your experiment...water bath to prevent evaporation and maintain the desired temperature. This also helps the water bath heat...water bath even if you are using disinfectants as described above in step 3. You will also need to maintain...
Showing: 921 - 940 of 994 results