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Showing: 921 - 940 of 998 results
  1. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...Analysis of a Plasmid You may also like... How to Design a Primer Diagnostic Restriction Digest Introduction...If you require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's...'s sequencing results as a reference for primer design. A good sequencing reaction will produce between...
  2. Water Bath Protocol

    Type
    Protocol
    ... of bacteria or fungi. There are disinfectants designed to be used in water baths with instructions on...bath on and set the appropriate temperature as described in your protocol. How to set the temperature will...Set up your water bath so that it will reach the desired temperature by the time you need it for your experiment...water bath to prevent evaporation and maintain the desired temperature. This also helps the water bath heat...water bath even if you are using disinfectants as described above in step 3. You will also need to maintain...
  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,...
  4. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...
  5. Transferable Skills: Negotiation

    Type
    Blog Post
    ...specific details about the individuals, each example provides a scenario where both people get something valuable...
  6. Immunocytochemistry

    Type
    Protocol
    ...This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody...antibodies to detect antigens in cells. Here we describe the basic steps for fixing and labeling cells ... per well. Allow the HeLa cells to grow to the desired density before labeling. Section 2: Fixing and ... container. Dilute the primary antibody to the desired concentration in antibody dilution buffer. Pro-...fluorescently-labeled secondary antibody to the desired concentration in antibody dilution buffer. Pro-...
Showing: 921 - 940 of 998 results