We narrowed to 935 results for: Kin

1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   ...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...

2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   ...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

3. Plasmids 101: Control Plasmids

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   ...results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...

4. Fluorescent Proteins 101: When GFP lets you down

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   ...Chartrand. "Using fluorescent proteins to study mRNA trafficking in living cells." Methods in cell biology 85 ...

5. CRISPR 101: Homology Directed Repair

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   .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

6. Quick Guide to Near-Infrared Fluorescent Proteins

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   ...lamps are better, and xenon lamps are the best for working in this spectral range. You can also check that...

7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   ... you chose for your experiment, but, if you’re working with plants, don’t be afraid to try your hand at...

8. Treating Muscular Dystrophy with CRISPR Gene Editing

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   ...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...

9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   ...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...2008). It can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa...

11. Plasmids 101: Restriction Cloning

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    ... using UV as it can cause damage to the eyes and skin. When running a gel for purification purposes it...

12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...inversion, and then lox sites excision, therefore locking the opsin into the correct orientation to be transcribed...

13. Antibodies 101: Validation

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    ...general suggestions given by the International Working Group on Antibody Validation (Uhlen, et al., 2016...

14. Antibodies 101: Introduction to Antibodies

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    ...green), CARD14 (red), and pNFkB (blue) in lesional skin from a psoriasis patient, showing an inflammatory...

15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...induced pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID...