We narrowed to 957 results for: REP

1. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...successfully infected the cell type of interest. Repeat rounds of infection and screening by PCR enrich...

2. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   ...Subsequent advances in fluorescent proteins have replicated many of the properties once exclusive to small-molecules...

3. A Career in Grants: How To Become a Grant Professional

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   Blog Post

   ...opportunity deadlines, and we ensure that grant reports are submitted and project evaluations are completed...

4. Transferable Skills Guide: External Collaborations

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   Blog Post

   ...But, as so often happens, one gets better with repetition. I learned to look at the event from the company...

5. Transferable Skills Guide: Career Planning Resources

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   ...of some of the resources you can use to better prepare for your career, and increase your chances of landing...

6. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ...scientists, students, and those from historically underrepresented groups. Through such mentoring programs, scientists...

7. Using Video to Share Your Science: We Share Science

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   Blog Post

   ...total view count and videos published, YouTube reported a total of 865,995 views for the 926 video abstracts...

8. Transferable Skills Guide: Time Management

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   ... can spend accomplishing small tasks (like... preparing plasmids to send to Addgene). Following my advice...

9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   ...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

10. Five Popular Model Organisms, Part 2

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    Blog Post

    ... ease of manipulation and their ability to be replicated. Because E. coli are easily propagated and have...

11. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post

    ...CRISPR, RNAi methods are often quicker and the repositories of validated small RNAs are vast and difficult...

12. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...7. https://doi.org/10.1038/s41557-023-01214-0. Preprint on bioRxiv: https://doi.org/10.1101/2022.09.26.509516...

13. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...for instance, may not transduce the adjacent norepinephrine neuron at all. There may be more than one serotype...

14. Finding Your Perfect Job After University

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    Blog Post

    ...lacking during degree courses; they never really prepare you for the amount of failure and problem solving...

15. Adeno-associated virus (AAV) Guide

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    Guide

    ...reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs). The Rep region encodes... Terminal Repeat, contains a Rep binding element (RBE) that serves as a binding site for Rep proteins ...plasmid Rep in trans The rep gene encodes four proteins that are required for viral genome replication and...Packaging plasmid (also known as Rep/Cap plasmid) — containing the Rep and Cap regions necessary for the...integration is mediated by proteins Rep78 and Rep68, which can bind to specific Rep-binding elements present in...DNA replication and packaging. 3' ITR (RITR) in cis Right Inverted Terminal Repeat, contains a Rep binding...Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the two ITRs with ...

16. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ... to evolve over time, which is bad news for repeatability and bad news for a screen. Because Addgene viruses...

17. A Guide to Getting Started in Undergrad Research

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    ...can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more involved...

18. Deep Dive: Statistical Tests (Comparisons)

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    ...Explorations in statistics: statistical facets of reproducibility. Adv Physiol Educ. 40(2): 248-252 (2016). 0.1152...

19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish ...

20. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    ...), and ability to metastasize. There have been reports of clinical trials going amiss and patient deaths...