We narrowed to 978 results for: REP

1. Getting to Know Expansion Microscopy

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   Blog Post

   ...ensure the expanded images are still a good representation of reality. Third, expansion results in dimmer...

2. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...successfully infected the cell type of interest. Repeat rounds of infection and screening by PCR enrich...

3. Plasmids 101: Secondary Nanobody Toolbox

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   ... genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration...

4. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...get maximum satisfaction out your work, and be prepared for new and exciting opportunities. Here are some...

5. Tips for arabidopsis transformation

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   ...RFP in the seed (Emami, Yee and Dinneny, 2013). Preparing plants for transformation Whatever plants you ...

6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...Subsequent advances in fluorescent proteins have replicated many of the properties once exclusive to small-molecules...

7. A Career in Grants: How To Become a Grant Professional

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   ...opportunity deadlines, and we ensure that grant reports are submitted and project evaluations are completed...

8. Transferable Skills Guide: External Collaborations

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   ...But, as so often happens, one gets better with repetition. I learned to look at the event from the company...

9. Transferable Skills Guide: Career Planning Resources

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   ...of some of the resources you can use to better prepare for your career, and increase your chances of landing...

10. Using Video to Share Your Science: We Share Science

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    ...total view count and videos published, YouTube reported a total of 865,995 views for the 926 video abstracts...

11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...scientists, students, and those from historically underrepresented groups. Through such mentoring programs, scientists...

12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

13. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...7. https://doi.org/10.1038/s41557-023-01214-0. Preprint on bioRxiv: https://doi.org/10.1101/2022.09.26.509516...

14. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...CRISPR, RNAi methods are often quicker and the repositories of validated small RNAs are vast and difficult...

15. Transferable Skills Guide: Time Management

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    ... can spend accomplishing small tasks (like... preparing plasmids to send to Addgene). Following my advice...

16. Five Popular Model Organisms, Part 2

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    Blog Post

    ... ease of manipulation and their ability to be replicated. Because E. coli are easily propagated and have...

17. Adeno-associated virus (AAV) Guide

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    Guide

    ...reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs). The Rep region encodes... Terminal Repeat, contains a Rep binding element (RBE) that serves as a binding site for Rep proteins ...plasmid Rep in trans The rep gene encodes four proteins that are required for viral genome replication and...Packaging plasmid (also known as Rep/Cap plasmid) — containing the Rep and Cap regions necessary for the...integration is mediated by proteins Rep78 and Rep68, which can bind to specific Rep-binding elements present in...DNA replication and packaging. 3' ITR (RITR) in cis Right Inverted Terminal Repeat, contains a Rep binding...Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the two ITRs with ...

18. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs). The Rep region encodes... Terminal Repeat, contains a Rep binding element (RBE) that serves as a binding site for Rep proteins ...plasmid Rep in trans The rep gene encodes four proteins that are required for viral genome replication and...Packaging plasmid (also known as Rep/Cap plasmid) — containing the Rep and Cap regions necessary for the...integration is mediated by proteins Rep78 and Rep68, which can bind to specific Rep-binding elements present in...DNA replication and packaging. 3' ITR (RITR) in cis Right Inverted Terminal Repeat, contains a Rep binding...Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the two ITRs with ...

19. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...for instance, may not transduce the adjacent norepinephrine neuron at all. There may be more than one serotype...

20. Finding Your Perfect Job After University

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    Blog Post

    ...lacking during degree courses; they never really prepare you for the amount of failure and problem solving...