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We narrowed to 1,050 results for: ins

Showing: 921 - 940 of 1050 results

  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...of interest, a medium copy RSF1030 origins or a low copy p15a origins should be used. Find further details...Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the WW2 ...orthogonal translation system to make phosphorylated proteins with one or more phosphorylation sites (Pirman...improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli XpS (Bacterial...other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express the...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...#68307) ) to generate the phosphosites with serine instead of phosphoserine (i.e., a “phosphorylatable” phosphosite...

  2. Lentiviral Prep Service

    Type
    Collection
    ...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178...with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ... ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962...and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells ...

  3. Fluorescent Protein Guide

    Type
    Collection
    ...Guide Fluorescent Proteins: Plasmids and Resources Addgene's plasmid repository contains thousands of fluorescent...fluorescent proteins. Use this guide to learn more about the many applications of fluorescent proteins and to...Fluorescent Proteins Luciferase Plasmid Collection Fluorescent Protein Kits Allen Institute for Cell Science...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...consisting of ORFs tagged with a variety of fluorescent proteins, as well as over 100 empty backbones. Additional...open-source, community-editable database for fluorescent proteins created by Talley Lambert (Link opens in a new...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ... almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often used...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable...for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (comprised of multiple...is important. However, type I-F CASTs favor insertion in the T-RL direction, with the right homology end...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection....Pooled Libraries Feedback Resources Solute carrier proteins (SLCs) are the largest family of transporters ...associations. Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...Superti-Furga CRISPR knockout library targeting human SLC proteins. Mouse SLC Knockout Library 141436 Superti-Furga...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including Markers...Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...with the mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. ...with concomitant expression of aggregation-prone proteins with the iPSC toolbox . Lam et al. bioRxiv. 2022...A foundation dedicated to finding a cure for Parkinson's disease through an aggressively funded research...

  7. Retrovirus Plasmids

    Type
    Collection
    ...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...pUMVC Contains MoMLV gag and pol . Use with an envelope plasmid. Bob Weinberg 12371 pCL-Eco Contains MoMLV...includes a reverse transcriptase and an integrase to insert the viral genome into a host cell. Scientists can...packaging genes Envelope plasmid — contains packaging gene env This collection highlights popular gamma-...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart 20672 MSCV-IRES-GFP MSCV...pol , and env . Inder Verma 35614 pBS-CMV-gagpol Contains MoMLV gag and pol . Patrick Salmon Return to top...

  8. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations using...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate for another...small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease protection...enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide...

  9. Worm Expression Resources

    Type
    Collection
    ...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids... vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids ...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids for building homologous repair...Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes...Caenorhabditis Genetics Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing...

  10. Synthetic Biology - Overview

    Type
    Collection
    ...Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden...environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain...terminators, repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab...Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning ...community-created BioBricks JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering Research...

  11. Rett Syndrome

    Type
    Collection
    ...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal D omain...or bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator...that can be differentiated into neurons Coriell Institute: Patient-specific lines (Link opens in a new window...Repositories (Link opens in a new window) Coriell Institute - cell line repository, including patient-derived...Additional Resources (Link opens in a new window) BrainSpan Atlas of the Developing Human Brain - includes... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

  12. Open Enzyme Collection

    Type
    Collection
    ...synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA...sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve...Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514 pOpen-... to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase...Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506 pOpen-Eco31I Eco31I... Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia... to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol...

  13. Viral Production

    Type
    Collection
    ...of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction of total...relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total.... Three to five days later, cell cultures are inspected for sterility. Transducibility Some viral vectors...virus from Addgene, read our detailed recipient instructions for next steps. Plan an experiment using our...to amplify a portion of the intended lentiviral insert. During this cultivation, cells are routinely checked...

  14. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource..., and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's Disease research. This collection is part of ...search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

  15. COVID-19 Resources

    Type
    Collection
    ...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...Llamas Required - Synthetic Nanobodies Against Membrane Proteins Return to top Pooled Libraries Pooled ...credit: Maya Kostman for the Innovative Genomics Institute The Coronavirus Disease-2019 (COVID-19) pandemic... plasmids expressing genes encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E;...Interaction Network Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins, along with key...create fusion proteins with your gene of interest. Plasmid... Plasmid Collections Fluorescent Proteins Empty Backbones...Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange Red Far-Red Near-Infrared...are prone to dimerization at high concentration. Proteins that are derived from GFP and contain the A206K...Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish/Xenopus/Worm...Expression Return to top Long Stokes Shift These proteins have a large gap between their excitation and ...

  17. Chemogenetics Plasmids

    Type
    Collection
    ...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...specific biological processes. Addgene's collection contains two main classes of chemogenetic receptors: Designer...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags Eligible for Viral Packaging...

  18. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are...collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive...promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources...2019). In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https:/...

  19. Promega Plasmid Collection

    Type
    Collection
    ...useful for detecting and tracking key drug target proteins....operational complexity. This unique collection contains plasmids developed by Promega scientists and designed...biology experimental workflows. The collection contains luciferase reporter vectors for sensitive gene...protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, SmBiT, and...SmBiT and expressed in live cells. When the two proteins interact, the subunits come together to form an...exceptional dynamic range can detect low-abundance proteins at endogenous levels in lysates or live-cells,... and inhibition in real time using full-length proteins or fragments. NanoBRET target engagement (TE) ...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences into the open reading...recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase...intersectional experimental design. The Deisseroth Lab maintains a Standard Operating Procedure (Link opens in ...transgenic mouse and rat lines. The Fenno Lab maintains a public folder (Link opens in a new window) of...additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies... Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock... Cre Yes Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock...
Showing: 921 - 940 of 1050 results