...overhangs by chewing back from the DNA 5’ end. Complementary DNA fragments can subsequently anneal to each... DNA Ligase - covalently joins the annealed complementary DNA fragments, removing any nicks and creating...opens in a new window) Rabe BA, Cepko C. (2020). A Simple Enhancement for Gibson Isothermal Assembly. bioRxiv...

...of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the aqueous DNA sample. Pro-Tip...remaining contaminant proteins and RNase A from the DNA sample. When phenol is mixed with the aqueous solution...

...bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow). It is, however...over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different...

...different samples without cross contamination as long as the tip is changed between samples. Tips can ...

...between samples. In addition to a DNA ladder standard, it is also a good idea to run an uncut sample of each...

... to the BSL-1 requirements. Conclusion Although simple, following appropriate PPE protocol goes a long...

...you can weigh out the reagents in batches. For example, if you need 300 g of sucrose, you can weigh out...

...water baths with instructions on the bottle, for example, number of drops per liter. Place a thermometer...

...liquid culture. Protocol Prepare liquid LB. For example, to make 400 mL of LB, weigh out the following ...