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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: mpl

Showing: 921 - 938 of 938 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... unknown sample (don’t forget to factor in that dilution when calculating the original sample concentration...science. General Considerations The ELISA can be completed in a single day or broken up over several days...is a suggested starting point. If your unknown sample’s absorbance falls above the range of the standard...concentration!). Sample Data Figure 1: The plate was coated with serial dilutions of human recombinant purified...

  2. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...tags or shRNAs to any vector (the procedure will simply differ in terms of primer design). Let's assume...CAATTG ). The bottom oligo will be the reverse compliment so that they can anneal. Top oligo: 5' - CATATG...bp We also need to include additional bases to complement the overhangs generated when digesting the vector...could leave off the 3’ G on each oligo (and the complementary C of the other oligo), but this would destroy...

  3. Video Library

    Type
    Protocol
    ...multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple tips...bands out of the agarose gel and purify the DNA samples. This is a commonly used technique for molecular... Your guide to the MTA process when requesting samples from Addgene Technology Transfer How to Order A...

  4. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ... strain Watch the Video! CRISPR Library Amplification Amplify CRISPR pooled-plasmid libraries Diagnostic...centrifuge to separate different components in a liquid sample Using a Light Microscope Learn about the parts ...protocols are the building blocks for many more complicated procedures. Name Description (Link opens in a...

  5. Protocol - Bacterial Transformation

    Type
    Protocol
    ...plasmid DNA for the purposes of storage and amplification. Higher efficiency cells are more important ...their own competent cells. This is a relatively simple procedure and is useful for performing low efficiency...so when higher efficiency is needed follow the complete protocol. Thaw the competent cells in your hand...outgrowth (for Ampicillin resistance it is ok to completely skip the outgrowth, for the other antibiotics...

  6. Immunocytochemistry

    Type
    Protocol
    ... the host species of the primary antibody. For example, use an anti-mouse secondary antibody for primary...optimal fixation method will vary depending on the sample type and the protein of interest. You may need ...expected and specific, include a positive control sample that you know expresses the protein, such as cells...the protein of interest, and a negative control sample such as cells that do not express the protein of...

  7. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...before you place the sample at -80°C. Frozen tubes are hard to write on and samples stored for long periods...streaking onto LB agar will prevent it from thawing completely and will improve the shelf life. It is very important...

  8. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... one of the four nucleotide bases. This is an example of a trace file from a high-quality portion of ...that these base calls are unreliable. This is an example of a trace file from a portion of a sequencing ... trace file will give you more information than simply looking at the bases provided by your sequencing...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...overhangs by chewing back from the DNA 5’ end. Complementary DNA fragments can subsequently anneal to each... DNA Ligase - covalently joins the annealed complementary DNA fragments, removing any nicks and creating...opens in a new window) Rabe BA, Cepko C. (2020). A Simple Enhancement for Gibson Isothermal Assembly. bioRxiv...

  10. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...that don’t cause disease in healthy humans, for example, non-pathogenic E . coli . BSL-2 is for labs that...your workspace with appropriate materials. For example, you may use 70% ethanol, quaternary ammonium, ...containers that can be autoclaved. Conclusion Although simple, following appropriate BSL-1 and BSL-2 protocol...

  11. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the aqueous DNA sample. Pro-Tip...remaining contaminant proteins and RNase A from the DNA sample. When phenol is mixed with the aqueous solution...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow). It is, however...over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different...

  13. Pipetting Protocol

    Type
    Protocol
    ...different samples without cross contamination as long as the tip is changed between samples. Tips can ...

  16. Weighing Reagents Protocol

    Type
    Protocol
    ...you can weigh out the reagents in batches. For example, if you need 300 g of sucrose, you can weigh out...

  17. Water Bath Protocol

    Type
    Protocol
    ...water baths with instructions on the bottle, for example, number of drops per liter. Place a thermometer...
Showing: 921 - 938 of 938 results