...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...

...Collection of guides that describe how popular scientific techniques work... Science Guides Addgene has created... created the following guides to help you start learning about various scientific concepts related to ...to our plasmid collection. Find links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics... sets. Read More Viral Vectors Find educational guides to learn more about the different types of viruses...

... it includes labs that work with agents associated with diseases in healthy humans. BSL-2 includes all...There are four biosafety levels. This protocol provides information for both biosafety level 1 (BSL-1)...important! Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository and...

...Cell type-specific delivery by modular envelope design. Nature Communications, 14(1), Article 1. https...

...addgene.org with any questions. Protocols Video protocol guides for standard laboratory procedures. How-To Videos...Protocols (Link opens in a new window) Video Link Description Related Page Making LB Agar Plates Create plates...Videos (Link opens in a new window) Video Link Description Related Page MTA Guide - Requests Your guide ...Videos (Link opens in a new window) Video Link Description Jessica Welch, PhD In this first installment ...

...magnifies it 10 times, and so on. The ocular lens also provides magnification and the power should be provided...provided on the microscope; often this lens provides 10x magnification. To determine the final magnification...that object! Equipment Light Microscope Prepared Slides (or other sample that can fit on a microscope stage...eyepiece so that you can see your image through both sides. Once you are happy with the lighting, use the coarse...

...be pushed away from the well comb or towards the sides/edges of the gel with a pipette tip. Place newly...Note: Loading buffer serves two purposes: 1) it provides a visible dye that helps with gel loading and ...Always Run to Red. Turn OFF power, disconnect the electrodes from the power source, and then carefully remove...-30 mins, replace EtBr solution with water and destain for 5 mins. Using any device that has UV light,...

...may also like... AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...2-3 minutes for cells to detach. Gently tap the sides of the CS2 to help detach the cells, add 200 mL ...for ~72 h. Harvest cells and media by tapping the sides of the CS5. Cells should detach easily. Transfer...

...library during amplification. This protocol is designed to be as general as possible but note that individual...activating pulse and follow all specifications described in the equipment manual. Immediately add 1 mL ...µl aliquots of cell/DNA mixture used (8 in the described protocol) using a new vented falcon tube for every...than Nanodrop, although most protocols have been designed with Nanodrop as the quantification method. If...

...Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol describes a general...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

...phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically... likely to ligate to itself rather than to the desired insert. If you are in this situation, it is important...reactions. Because ligase buffer contains ATP, which degrades upon freeze/thaw cycles, it is a good idea to ...

...maximum possible setting on the pipette, set the desired volume of the liquid on the pipette. Depending ...of the pipette. Select the pipette tip that is designed to fit your pipette. To load a tip onto a pipette...from the container making sure not to touch the sides of the container with the pipette. Discard the pipette...

...Last Update: Feb. 8, 2018 Equipment Desktop microcentrifuge Desktop vortexer Vacuum (optional) Reagents... several μg/μL. The protocol below is meant to describe the general procedure for purifying plasmid DNA...

...with Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...dilutions and pick the population that has the most desirable level of expression. Over time, transgene expression...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

...Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes how to...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

...samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

...and with a clean, sterile razor blade, slice the desired DNA fragment from the gel. Notes: To protect the...down on the UV box and trim the top, bottom and sides with the razor blade. This is especially important...

...Lentivirus Production You may also like... Viral Vector Guides Mol Bio Protocols Introduction This protocol can...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

...Ultracentrifugation You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction...preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules. The...

...-agar 1 L Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which...next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the first plate...