We narrowed to 959 results for: LAT;

...silence the same neuronal population, or activate different neural populations at distinct times. Temporal... Two factors determine which neuronal population is manipulated in a given experiment: the expression ... integrates optics and genetic engineering to manipulate cells (frequently neurons) and their governing...neurons upon exposure to light through direct stimulation of ion channels. Naturally occurring channelrhodopsins... nonspecific cation influx into the cell when stimulated. Channelrhodopsin-2 (ChR2), the first widely ...Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type halorhodopsin, known...High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from...

...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...single strand of RNA contains packaging genes, regulatory genes, accessory genes, and the long-terminal...Wild-type lentiviruses additionally require the regulatory genes tat and rev , along with virus-specific... — contains packaging genes gag and pol , and regulatory genes tat and rev ; separated into two plasmids...plasmid — contains entire viral genome (packaging, regulatory, and accessory genes), only envelope removed ...can more specifically dictate the target cell population and reduce cellular toxicity. Common Uses of ...(FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information on generating...

...Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol....Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering Plasmid...

...doors. For those with latex allergies, be aware that some gloves may contain latex. Consider nitrile gloves...

... water, not tap water, to prevent salts from accumulating on the surfaces of the water bath as the water... your experiment. Water baths tend to heat up relatively slowly. Place the water bath cover on the top...

.... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

...be in the liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...

...Daniel Voytas 93 additional plasmids including regulatory elements, coding sequences, CRISPR parts GreenGate... for assembling large DNA fragments based on methylation-switching. 4G-Cloning Plasmid Kit Bacterial Expression...Expression, Mammalian Expression Stephan Gruber Platform for rapid assembly of expression vectors for multi-subunit...TALE-TFs) for genome editing and transcription modulation applications Golden Gate TALEN and TAL Effector...TALEN and TAL Effector Kit (Voytas and Bogdanove). Platinum Gate TALEN Kit Genome Engineering, TALEN Takashi...Yamamoto Plasmids for construction of highly-active Platinum TALENs for use in a wide variety of target organisms...

...Workflow Timeline Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand and...

...name, pooled libraries are supplied as a mixed population of plasmids in a single tube. Pooled libraries...may lead to false positives or false negatives in later experiments, and can also negatively affect data...every plasmid is adequately represented in the population, you’ll need to infect many more cells than the...the cells die, removing their plasmids from the population, and only a small fraction survive. After the... serves as a non-selected control. These two populations are then sequenced using NGS to determine which...

...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...(FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell line. You...best practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR in cis...

...molecular cloning, scientists can amplify and manipulate genes of interest and then insert them into plasmids...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...

...any of our eBooks to learn about popular topics related to our collections. Titles include: Plasmids 101...Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics include: Genome...

...start learning about various scientific concepts related to our plasmid collection. Find links to the guides...optics and genetic engineering to measure and manipulate cells (frequently neurons) and their governing...

...balance. This will help you remove it from the scale later on as otherwise, the paper could stick to the surface...

...sequence is used, plus a minimum of 18 bp of your template sequence. 5' and 3' primers will have different...

...lab. Ensure that a designated chemical waste accumulation site is present, and that you have received ...

...gel. Pro-Tip If you will be purifying the DNA for later use, use long-wavelength UV and expose for as little...

...you need to know about plasmids, from parts, nomenclature, and uses to common promoters and cloning techniques...