Addgene

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Showing: 941 - 960 of 1015 results

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What's New in CRISPR - December 2019

Type

Blog Post

... they’ve engineered SECURE adenine base editor variants using a protein-truncation strategy with reduced...
Scientist Networking: What is an Informational Interview?

Type

Blog Post

...opportunities for advancement, challenges and disappointments. These more personal questions can elicit the...
X-CHIME: Context Dependent Germline Knockout in Immune Cells

Type

Blog Post

...transduced stem cells can be implanted in irradiated recipients to create mice with gene knockouts in their immune...
Inside Addgene's 10 Year Anniversary Gala

Type

Blog Post

...entire night was when Melina and Ken called their parents up to the podium to be recognized. John and Stephanie...
What's New in CRISPR - March 2020

Type

Blog Post

...CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout et al.,...
Cre-ating New Methods for Site-specific Recombination in Drosophila

Type

Blog Post

...recombinases were also active in mammalian cells. In experiments, B3 and KD were shown to be functional in cultured...
London Calling - Addgene’s New Europe Office in London

Type

Blog Post

...information and I believe that a lot of European students and researchers are craving, like I was, for a...
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

Type

Blog Post

...protein-coding genes as well as non-coding genetic elements.” These lentiviral libraries and their demonstrated...
Antibodies 101: Affinity Tags

Type

Blog Post

...chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ...
Antibodies 101: Fc Effector Functions

Type

Blog Post

...classical complement pathway, starting a cascade of events that results in the pores being opened in the pathogen...
CRISPR 101: Drug discovery and CRISPR

Type

Blog Post

... line knock-ins of patient mutations which are variants of unknown significance – mutations with unclear...
New Viral Vectors - Fall 2024

Type

Blog Post

... prep packaged in the AAV9-X1.1 serotype. In rodents, AAV9-X1.1 enables expression in brain endothelial...
Build Your CRISPR Vocabulary

Type

Blog Post

...labs have created Cas9 enzymes with looser PAM requirements, which are available from Addgene! RNA crRNA...
Plasmids 101: Positive and Negative Selection for Plasmid Cloning

Type

Blog Post

...an essential amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical...
Five Questions to Ask If You're Deciding Between a Master's or PhD Program

Type

Blog Post

.... Don’t discount job postings looking for PhD applicants since many will also accept applications from...
Viral Vectors 101: Parts of the AAV Packaging Plasmid

Type

Blog Post

...plasmid and take a look at each of its parts. Key elements of the AAV packaging plasmid The packaging plasmid...
Bringing Sustainable Practices to the Lab: Recycling

Type

Blog Post

...boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will likely end up in a ...
Oh, The Places You Can Go: Careers in Science Communication - Science Writing

Type

Blog Post

...what types of jobs you are interested in in the comments. They could be featured in future career blog ...
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

Type

Blog Post

...of my time meeting with and talking to graduate students, post-docs, and PIs. I won’t pretend that that...
Management for Scientists: Managing vs. Leading

Type

Blog Post

...successful because you are not fulfilling the requirements of the current task. Most of us start as doers...

Showing: 941 - 960 of 1015 results

Results per page:

What's New in CRISPR - December 2019

Scientist Networking: What is an Informational Interview?

X-CHIME: Context Dependent Germline Knockout in Immune Cells

Inside Addgene's 10 Year Anniversary Gala

What's New in CRISPR - March 2020

Cre-ating New Methods for Site-specific Recombination in Drosophila

London Calling - Addgene’s New Europe Office in London

Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

Antibodies 101: Affinity Tags

Antibodies 101: Fc Effector Functions

CRISPR 101: Drug discovery and CRISPR

New Viral Vectors - Fall 2024

Build Your CRISPR Vocabulary

Plasmids 101: Positive and Negative Selection for Plasmid Cloning

Five Questions to Ask If You're Deciding Between a Master's or PhD Program

Viral Vectors 101: Parts of the AAV Packaging Plasmid

Bringing Sustainable Practices to the Lab: Recycling

Oh, The Places You Can Go: Careers in Science Communication - Science Writing

Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

Management for Scientists: Managing vs. Leading

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