We narrowed to 955 results for: tes

1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting...

2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   ...doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang D, Baltes NJ, Paul JW III, Tang X, Zheng X, Voytas DF, Hsieh...

3. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   ... the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling it to bind...

4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   ...transduce neurons for retrograde labeling, as well as astrocytes in the CNS (Han et al., 2023), and has also been...

5. Typing CRISPR Systems

   Type

   Blog Post

   ... clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is already on...

6. Science Guides

   Type

   Guide

   ...experiment. Read More Optogenetics Optogenetics integrates optics and genetic engineering to measure and...

7. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   ...descriptions first before entering the data. This step associates an Addgene ID with your plasmids, and adding ...

8. Guide to Using Pooled Libraries

   Type

   Guide

   ...maxiprep). If delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled...

9. Sequencing Primers

   Type

   Guide

   ...Forward pAd-CMV GCTAGAGATCTGGTACCGTC For cloning sites after SalI in pAd-CMV vector Forward pBABE 3' ACCCTAACTGACACACATTCC...

10. Protocol - How to Design Primers

    Type

    Protocol

    ...also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process...

11. Protocol - How to Create a Bacterial Glycerol Stock

    Type

    Protocol

    ...2 mL screw top tube or cryovial and gently mix. Notes: Make the 50% glycerol solution by diluting 100%...

12. Protocol - How to Streak a Plate

    Type

    Protocol

    ... Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...

13. Fluorescence Titering Assay

    Type

    Protocol

    ...lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one ...

14. Lab Safety for Biosafety Levels One and Two

    Type

    Protocol

    ... in a final concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste...

15. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ...PES filter or centrifugation at >500 x g for 5 minutes. Do not use the medium if the cells are overly ...