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Showing: 481 - 490 of 997 results
  1. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...chalk talk is effectively a million dollar sales pitch. Given this large sum, one might expect that applicants...accept you as a colleague and give you a million dollar startup package. There are three steps to chalk...resources required to feasibly accomplish the aims and clarify what type of aim you are describing. It's easy ...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...
  2. Hot Plasmids - August 2020

    Type
    Blog Post
    ...from purified recombinant proteins at small and large scale.  This new technique involves selection in...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...affinity purification required! The team used other molecular techniques to introduce time modulation of the... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...PHP.V1 that exhibits efficient transduction of vesicular brain cells. Find this AAV at Addgene New serotypes...
  3. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...their communication with gene promoters. Cellular and Molecular Life Sciences, 78(19–20), 6453–6485. https...systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX...investigator at the Allen Institute in the department of Molecular Genetics. References and Resources References...Zhou, T., Mortrud, M., Yao, S., Siverts, L. A., Larsen, R., Gore, B. B., Szelenyi, E. R., Trader, C., ...
  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    ..., which is not well protected by Cas9. This is similar to the proposed mechanism for engineered pegRNAs...related voltage indicator Ace2N-mNeon also led to similar improvements in performance.      Figure...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...
  5. Tips for Writing a Good Cover Letter

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    Blog Post
    ...out.  In some fields, a cover letter is vital, particularly in fields where communication and writing are...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...working for that organization or group. This is particularly important for labs and nonprofits, who tend ... did, and state that it would help you manage a large number of files as part of the job. Structure of.... It can also convey your excitement for that particular job, your commitment to the organization's mission...
  6. Viral Production at Addgene

    Type
    Blog Post
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination ...silver staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid...lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a...virus protocols. Browse our plasmid collection of popular lentiviral vectors. This article was written by...
  7. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... pCas/pTargetF system has been one of the most popular genomic editing systems in E. coli. However, some... linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or directly ligated...Academy of Sciences (CAS) Center for Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences since December 2020. His research... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate...
  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more...young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...getting a phone interview! If you don’t have a particular employer in mind, you may consider starting with...pages. Make yourself visible on LinkedIn and other popular job site webpages. Importantly, find a good mentor...
  9. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...often used to study the biology surrounding a particular gene (although it has been quite successfully...mutation and need to focus your attention to that particular region. No matter where you are looking, you ...the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA expression), one gRNA at a time...Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ...
  10. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...research. Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...test chamber is used in which a nose-poke at a particular hole (the active hole) induces laser stimulation...without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation and is a sufficient... happens. You never know how stimulation of a particular brain region may affect the animal; stimulation...
Showing: 481 - 490 of 997 results