Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 971 results for: lars;

Showing: 481 - 490 of 971 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...transformants. Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags.... John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification in...at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome... to the collections featured above, many other modular yeast tagging systems have been developed in the...

  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is a very common...continues to work on further improvements. In particular, they’re focused on generating variants with ...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...can help reduce off-target activity. Deleting a large region of the genome by removing the sequence between...Transcript Multiplexing Plasmids: These plasmids are similar to the Csy4 polycistronic system described above...Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for rapid combinatorial mammalian... genome editing in monocot and dicot plants.” Molecular Plant 8(8) (2015): 1274-1284. PubMed PMID: 25917172.... 11. Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays...

  4. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...control over neuronal activity. While they serve similar purposes, both techniques offer researchers different...have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel...with light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent intracranial...

  5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...Problems with Chip-seq ChIP-seq typically requires a large amount of input material, cells or tissue, to produce...with cell culture, because it is easy to harvest large quantities of cells. However, when working on specific...false-positives, as proteins may appear to be binding at particular genomic sites when in fact they are just non-...situations when it is technically challenging to collect large numbers of cells or when a high degree of signal...

  6. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...Plasmids with Addgene Find Molecular Biology Protocols and Videos Find Other Molecular Biology Resources ...paper and then another figure in another paper. A large fraction of readers cover the same ground again ...allows you to make meaningful collections from a large section of open access life sciences data. You can...

  7. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...easy to navigate. We also updated some our most popular CRISPR blogs to keep them current. Here are some...plasmid class covered on that page. To help you find popular plasmids, we’ve also added four new plasmid pages...mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been requested...only the specified RNA target. Bonus: It displays similar on-target activity to siRNA, but with far fewer...

  8. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...General Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At... tissues. sdAbs can also be used to manipulate cellular functions, like in this early example we highlighted...

  9. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...researchers and ready access to stably archived large-scale datasets by computational biologists. ...genome-wide datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it ... interest. In addition, users interested in a particular disease, cell type or drug treatment can browse...used to search the entire CRISPR database for particular screens of interest.   Figure 3: Each ...
Showing: 481 - 490 of 971 results