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We narrowed to 1,144 results for: Gene

Showing: 961 - 980 of 1144 results

  1. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ... hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene. What is initiative... Once in-person, peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking...almost every career talk I present, I’m asked what Addgene “looks for” when hiring a scientist. Of course,... comments below! Additional resources on the Addgene blog Read our science career blog posts Check ...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate... Generate Insertion and Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...Fluorescent proteins (FPs) are the gold standard for genetically-encoded FRET donors (Fig. 1). Scores of FP variants...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

  4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...Fatima's work and keep checking back in with the Addgene blog for future award opportunities. Xin Zhou:...when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked...Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation...Chemical Biology! Additional Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein Biosensors Browse Plasmids...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...specifically how dry ice can affect virus from Addgene. Bottom line: there’s good news and there’s bad... results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What we did To... recommend that once you receive your AAV from Addgene, you store the AAV at -80°C for 96 hours prior ... PMCID: PMC4420252 Additional Resources on the Addgene Blog Learn How You Can Use the Viral Service Tips...Your Lentivirus Preps Additional Resources on Addgene.org Check out the Viral Service Find Virus Protocols...

  6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...suited than another. Additional resources on the Addgene blog Read more about molecular biology protocols...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

  7. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...external collaborations Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ...efforts to shift scientific culture towards a more generous and rewarding way of making science.   How...currently based in Spain and works in the field of genetics and cancer. You can follow her on Twitter at @...mahesa2504         Resources Additional resources on Addgene's blog Check out our blog post about remote collaborations...

  8. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication...yourself how every piece of feedback you receive generalizes.  If someone suggests a change to a slide, think... science communication, including the teams at Addgene, ComSciCon, and many more great organizations. ...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...expression and protein interactions, including epigenetic modifications. But it's not an approach for the... this investment can well be worth it once you generate your data. However, it may be a good idea to add...  References and Resources More resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies...

  10. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics:...antibody for a…less sensitive moment.   Fig 1: Blugene fading into darkness because they accidentally ...accidentally left their antibodies out. It's okay, Blugene!    Buffers  You know what temperature to store your.../help/spectral-overlap/   More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies...

  11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... Labeling in Flow Cytometry Resource on addgene.org Addgene's Antibody Guide rAb Affinity Purification...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...Harvard Medical School.   More resources on the Addgene blog Antibodies 101: Flow Cytometry Conventional...Purification Protocol Video rAbs in the Addgene repository...

  12. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    .... Different setting, different population, new genetic variation, or different handling can all be important... tested with a new experiment; and hypothesis generation, in which data are investigated to look for unexpected...trends or differences between groups. Hypothesis generation is critical for advancement, but we are too often...using hypothesis testing statistical tools that generate a p-value.  As an example, if you hypothesize ...clear distinction between hypothesis testing and generation, can be sure that results will be reported regardless...

  13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three...PMCID: PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...

  14. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene Blog Which...the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural affinity and...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

  15. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...leading up to this date, as discussed in a previous Addgene blogpost and more recently in a paper published...There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel...Research @FORsymp.   Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor...

  16. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced in animals...instead of via recombinant DNA, they can’t be genetically tagged and instead are conjugated to reporters...monoclonal antibodies, but they are laborious to generate and costly to share. What is a RANbody? A RANbody...PubMed PMID: 29440485. Additional Resources on the Addgene Blog Learn more about using the secondary nanobody...Check out this post. Additional Resources on Addgene.org More nanobody expression plasmids can be found...

  18. Summer SciComm: Preprints

    Type
    Blog Post
    ...attendees to read and comment online! Want the general public (say, policymakers or community organizations...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...more openly — which is also our mission here at Addgene! Along with other open-science organizations, we...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...because it helps me see problem solving as a generalizable process.  Step One: Identify the problem ...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  20. To Each HIS Own

    Type
    Blog Post
    ...  Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to ..., Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant...
Showing: 961 - 980 of 1144 results