We narrowed to 1,106 results for: Ide
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TypeBlog Post...sequencing projects were launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year...Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links...
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Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van ... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi... -
Summer SciComm: Science Storytelling
TypeBlog Post...make them decide to read (or listen or watch) further. Second, the introduction needs to provide enough ... -
Antibodies 101: Epitope Availability
TypeBlog Post...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post... Di Ventura on Twitter, you might have seen the video of her singing about the paper recently published... transcription from the PBAD promoter. “AraC is widely used in synthetic biology, biotechnology, and almost... -
New Viral Vectors - Winter 2025
TypeBlog Post...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ... -
What the HEK?
TypeBlog Post...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise...Addgene's viral service Find viral protocols and videos Find AAVs for your research ... -
Another Pathway into Cells: iTOP
TypeBlog Post...cells to take up protein directly? It’s not a new idea, but it comes with a few caveats. The most common...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...control AAV inventory to give scientists access to a wide array of promoters and fluorophores for validating...Addgene's viral service Find viral protocols and videos Find AAVs for your research ... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post... schedule tools How to write a lot: A practical guide by Paula J. Silvia This book has tips for how to...suggestions. De-jargonizer The De-jargonizer can identify jargon in your writing. While it’s not possible... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...Professor Max Krummel from UCSF showed some impressive videos of these interactions (Ref 3). You can find useful...tips on in vivo imaging in our Fluorescent Protein Guide. Throughout the conference aspirin and the use of... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et... protein. This technique is very similar to the widely used FRET, but it eliminates the need for an excitation... -
Plasmids 101: Inducible Promoters
TypeBlog Post...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide and Plasmids ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...inhibits over 18,000 human genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral...Addgene's viral service Find viral protocols and videos Find AAVs for your research ... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...sequencing primers, and hand deliver them to our Sanger provider. These results come back within 8-12 hours and...and meet our QC standards. If any concerns are identified, we can quickly put a hold on all orders and ... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...messengers. Optical control of kinase activity can provide greater spatiotemporal resolution than pharmacological...vanishing, and pa= photoactivation) domains on either side of the active site of a constitutively active MEK1...