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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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  1. Lentiviral Vector Guide

    Type
    Guide
    ...infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic material...of experiments being performed. Your biosafety office can provide more information on your institution'...they are different viruses and require different isoforms of these packaging components. Therefore, lentivirus...ccdB-resistant strain is necessary. For more information on cloning and working with plasmids, visit Addgene...will have the lentiviral genome and the genetic information encoded by that genome. Antibiotic resistance...GFP and later expanded into a cell line. More information on generating cell lines with lentiviral vectors... Addgene’s Biosafety Resource Guide for more information (including tropism and viral genome percentage...

  2. Guide to Using Pooled Libraries

    Type
    Guide
    ...your library from Addgene, check the library information to see if it should be amplified before you conduct...in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS) on a control sample...library Apply selection; most cells will survive Perform NGS on the surviving cells Compare NGS results ... Company Resources Science Exchange Scientist (formally Assay Depot) Resources Find additional resources...amplification protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse...

  3. Educational Resources

    Type
    Guide
    ...Selection What is a Plasmid? Genetic Code Strain Information Molecular Cloning Techniques Protocols Find written...virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning Creating Bacterial Glycerol...

  4. Addgene's eBook Collection

    Type
    Guide
    ...find tips for choosing a fluorescent protein; information on FRET, biosensors, and monitoring protein localization... | First Edition About The guide includes information on the many career options in science, advice...

  5. Promoters

    Type
    Guide
    ...molecule binds to the repressor, it changes its conformation, preventing its binding to the operator and ...
Showing: 961 - 965 of 965 results