We narrowed to 160 results for: ALC

1. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   .... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...

2. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   Blog Post

   ...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...

3. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   Blog Post

   ...genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated between...

4. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   Blog Post

   ... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...

5. Plasmid Cloning by PCR

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   Blog Post

   ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

6. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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   Blog Post

   ...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...

7. Plasmids for Stem Cell Research

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   Collection

   ...fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Mol...

8. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   Blog Post

   ... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...

9. FPbase: A new community-editable fluorescent protein database

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   Blog Post

   ...feature filter spectra and collection efficiency calculations.  With ~80 FP spectra (and rapidly growing, ...

10. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...inhibitor that stalls ribosomes after initiation, to calculate a ribosome translocation rate of ~3.5 codons/second...

11. Addgene Depositors Get More Citations

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    Blog Post

    ... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...

12. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post

    ...wealth distribution” curve, or Lorenz curve. By calculating the area under the curve (AUC), one can gain ...

13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post

    ...algorithms are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al...

14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    Blog Post

    ...) respectively. The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized...

15. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ...wild type target in each sample can be quickly calculated (details in [2]) and can be used to quantify ...

16. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target...

17. CRISPRainbow and Genome Visualization

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    Blog Post

    ...physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously...

18. Hot Plasmids - October 2020

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    Blog Post

    ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

19. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

20. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,...