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Showing: 121 - 150 of 275 results

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses ...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our...

  3. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch... switch to droplet digital PCR to quantify AAV titers. Get the details on the methods in this blog post...

  5. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...diseases. The researchers injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a...Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal...viral service Find viral protocols and videos Find AAVs for your research ...vigilance. A recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor...specifically activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver...

  6. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print...

  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...are named after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And... Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... it can be packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter... smaller than SaCas9 and is also compatible with AAV delivery. NmCas9, another small ortholog, displays... Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page Resources...

  11. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and 5HT sensors New AAV tools to selectively...from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps  ...

  12. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally manipulate...tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here...27798629 Listen to Our Podcast Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that...

  13. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Adeno-Associated Viral (AAV) Plasmids Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids...Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org Adeno-Associated Viral (AAV) Vector Guide...Transfection AAV Production in HEK293T Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration...AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 James M. Wilson 112865 pAAV2/9n AAV9 AAV packaging ...rh10 capsid James M. Wilson 81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid, expressing Rep2...Plasmids AAV Helper Plasmids Adeno-associated viral (AAV) vectors offer precise delivery of genetic material...These genes are not present in the AAV genome, but are required for AAV viral replication. This collection...

  14. How Addgene Creates Content

    Type
    Blog Post
    ...Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website, Data Hub...enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists to easily...

  15. Brain Armamentarium

    Type
    Collection
    ...Alias: pAAV_WDP0003_GCaMP6f Gordon Fishell Viviana Gradinaru 213941-AAV1 pAAV_BiPVe3_ChR2_mCherry AAV construct...Alias: pAAV_WDC0004_GCaMP6f Gordon Fishell Viviana Gradinaru 213937-AAV1 pAAV_BiPVe4_ChR2_mCherry AAV construct...Alias: pAAV_WDL0003_jGCaMP8m Gordon Fishell James M. Wilson 213917-AAV1 pAAV_BiLAMP5e3_GCaMP6f AAV construct...Alias: pAAV_YWE4.2_GCaMP6f Gordon Fishell Viviana Gradinaru 213856-AAV1 pAAV_BiVIPe4_ChR2_mCherry AAV construct...Alias: pAAV_S9E27_GCaMP6f Gordon Fishell James M. Wilson 213833-AAV5 pAAV_BiCHATe27_GCaMP6f AAV construct...Alias: pAAV_S9E10_GCaMP6f Gordon Fishell Viviana Gradinaru 213815-AAV1 pAAV_BiSSTe10_ChR2_mCherry AAV construct...Science , Bosiljka Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part of our Viral...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA...Project 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Douglas Kim 104489 pGP-AAV-syn-jGCaMP7b-...Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Douglas Kim 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE Project 162375 pGP-AAV-syn-jGCaMP8m-WPRE...

  17. Brain Initiative Collection

    Type
    Collection
    ...vectors. Jonathan Ting 172907-AAV1 pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor...GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...

  18. Recombinases AAV Preps

    Type
    Collection
    ...Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps that encode site-specific...Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore...with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40... 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Stanley Thomas Carmichael 214848 AiP13780: pAAV-AiE0743m...

  19. Serotype Testing AAV

    Type
    Collection
    ...Service AAV Serotype Testing AAV Viral Vector Packaging Service: Serotype Testing AAV These AAV encode ...for serotype testing: AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and AAV retrograde. Viral...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid... Cart AAV9 37825-AAV9.T 20 µL $ 150 Add to Cart AAV11 37825-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde...150 Add to Cart AAV11 50465-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde 50465-AAVrg.T 20 µL $ 150 Add...have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description...

  20. Caltech Systemic Capsids

    Type
    Collection
    ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-...GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...scientific community. Text Description for the AAV Decision Tree The AAV decision tree graphic above helps you ...

  21. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...and shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one...my lab mate’s with her project. Unfortunately the AAV didn’t give us the results we hoped for, but it did...

  23. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce...

  24. Biosensor AAV Preps

    Type
    Collection
    ...Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our AAV that express ...pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 5, 9, rg* GENIE Project 162379 pGP-AAV-syn-FLEX-jGCaMP8f-WPRE...169258 AAV-hSyn-Soma-jGCaMP8f Syn soma-jGCaMP8f none Constitutive 1, 9, rg* Marianne Fyhn 176750 AAV-CamKIIa-jGCaMP8f-WPRE...176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Loren Looger 176756 AAV-EF1a-jGCaMP8f-WPRE...pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE Project 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg* GENIE Project 169256 AAV-hSyn-Soma-jGCaMP8s...176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 9, rg* Loren Looger 176752 AAV-CamKIIa-jGCaMP8s-WPRE...

  25. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed targets. ...from Neumann et al. 2024. The team showed that AAV-delivered CHARM could silence the expression of prion... (2024). Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic...

  26. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing...facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline modification...especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important...with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively...

  27. AAV Viral Preps

    Type
    Collection
    ...Vector Packaging Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful...Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech... one of eight serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, or AAVrg). For more information, visit...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro...protection levels. High quality and high titer in-stock AAV preps are affordable, available in small volumes,...

  28. Addgene: The First Twenty Years

    Type
    Blog Post
    ...vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing supportive...be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...
Showing: 121 - 150 of 275 results