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We narrowed to 386 results for: nans

Showing: 121 - 150 of 386 results

  1. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

  2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...pathophysiological complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation...renewable sensor, a bacterium that can sense contaminants and emit light as a response. To create this...fluorescent protein transcription in response to river contaminants and transform these plasmids into a bacterial...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are used. For predominantly retrograde tracing, albumin protein labelled...PMC2600441. Foust, K.D., Nurre, E., Montgomery, C.L., Hernandez, A., Chan, C.M. and Kaspar B.K. (2009). Intravascular...2009). Comparison of transduction efficiency of recombinant AAV serotypes 1, 2, 5 and 8 in the rat nigrostriatal...motor neurons after intramuscular delivery of recombinant AAV serotype 6. Gene Ther 17, 141–146. PubMed...

  4. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...field-based software product manager at Oxford Nanopore Technologies. If the first thing you hear when...streamlined manner. Now, in my current role at Oxford Nanopore Technologies, I work with our customers in the...currently a Field-based Product Manager at Oxford Nanopore Technologies for the North America region. She...

  5. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...embryos produced were not being used to establish a pregnancy, the researchers were able to examine each individual...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...optogenetics, and chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application of AAV is...the deliverable DNA) are removed, resulting in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...

  9. CRISPR in the Clinic

    Type
    Blog Post
    ... CRISPR’s therapeutic potential in mice with a dominant cataract disorder caused by a single copy of a...have experienced hiccups (either clinically or financially), and others we just don’t have the time to talk...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...

  10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...immunogenic profile. AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral... responses to adeno-associated virus and its recombinant vectors." Gene therapy 10.11 (2003): 964-976....

  11. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Watch the Video! Water Baths Proper water bath maintenance and use Watch the Video! Pipetting Learn about...produce antibodies Antibody Purification Purify recombinant antibodies using Protein A or Protein G columns... Stain Determine purity and concentration of recombinant antibodies Watch the Video! Western Blot Separate...

  12. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic gRNA, is incubated...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4...

  13. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under conditions commonly...Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct...Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent...

  14. An Introduction to Adenovirus

    Type
    Blog Post
    ...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...from the genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the...natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral vectors all have a genomic...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Cloin BMC, Finan K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label density...

  16. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... genes required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball...hMDH1). They then expressed the original and recombinant enzymes in bacteria, purified them to near homogeneity...complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119...

  17. Plasmids 101: Protein tags

    Type
    Blog Post
    ...to aid in the expression and purification of recombinant proteins. Tags for stability and solubility ...hurdles to overcome in order to overexpress a recombinant protein? It is not generally in a cell’s best...glutathione S-transferase (GST), which can be fused to recombinant proteins for one-step purification with glutathione...

  18. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...has created an automated tank for feeding and maintenance that had the audience gasping. Animal husbandry...the number of plants you need to grow to get a recombinant because this process is expensive. They are using...fluorescent indicator system to rapidly identify recombinants. Arthropods (Jason Rasgon) This lab is developing...

  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ..., Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose or magnetic...their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed, which can...on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...non-specific binding or false positives.  If testing a recombinant protein, especially one with tags, be sure to...the endogenous protein, since folding of the recombinant protein may differ from the native form, which...addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...

  21. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...results in the production of air-filled protein nanostructures called gas vesicles, which scatter sound waves... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...Maresca, D. et al. Nonlinear ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, ...

  22. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...implemented and from that, develop better software.  Maintenance  We’re not just about developing new features...: we take time during each sprint to do some maintenance work on our code. One way to look at this is ...continuing to add new features.  Another area of maintenance is agreeing to the names of the things in our...

  23. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...to the daily news briefing, to quick notes, to finances, to flights & ordering food - everything is covered...significant barrier to ELN adoption. This includes financial outlay, staff hours, troubleshooting, and the ... long-term use is likely to require on-going maintenance and support.” Besides that, academic institutions...

  24. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR...11012), and counterstained with DAPI. New recombinant antibodies targeting Glypicans By Ashley Waldron...) recently released a set of highly-specific recombinant antibodies against three of the six human glypican...

  25. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Central PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction and expression of genes...expression and largely defines the amount of recombinant protein that will be produced. Many commonly-...

  26. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...components can be electroporated into the oviduct of pregnant females, a technique called Genome editing via...injection into either an oocyte or into the oviduct of pregnant females (Yoon et al., 2018, Mizuno et al., 2018...in vivo genome editing in mouse embryos using recombinant adeno-associated viruses. Nat Commun 9: . https...

  27. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...and should not be confined by birth country or financial means. Instead, talent should be realized through...globalized environment re-emerging from international financial struggle, our new generations feel thrown, to ...twitter @zcastan or on linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard...

  28. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...When to Use Them Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide... software Reagents Purified antigen Purified recombinant antibody Isotype control antibody HRP-conjugated...plate was coated with serial dilutions of human recombinant purified Desmin (blue) or a negative control ...

  30. Important Considerations When Using AAVs

    Type
    Blog Post
    ...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...-limiting step for efficient transduction by recombinant adeno-associated virus vectors." Journal of virology...10. Wu, Jianqing, et al. "Self-complementary recombinant adeno-associated viral vectors: packaging capacity...
Showing: 121 - 150 of 386 results