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We narrowed to 1,023 results for: Cat

Showing: 161 - 200 of 1023 results

  1. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...Look up the IDs of particular Penn Vector Core catalog items. Virus-associated DNA Each viral vector service...

  2. Worm Expression Resources

    Type
    Collection
    ...Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. ...consortium of biologists and computer scientists dedicated to providing the research community with accurate...

  3. p53 Pathway

    Type
    Collection
    ...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific...

  4. Arf GTPase Family

    Type
    Collection
    ...involved in GTPase signaling activity. Figure 1: Locations of ARF superfamily members. Figure from Szutl,...28964 770 Mammalian (pcDNA3, pEGFP) GAP Git2 (PKL, CAT2) 9815 759 GAP ELMOD1 55531 334 Bacterial (pGEX4T...

  5. Viral Vectors

    Type
    Collection
    ...of certain genes that are required for viral replication. Thus, as a safety feature, viruses used in research...Instead, the genes that are required for viral replication are usually divided among many plasmids, such...

  6. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...extensively validated for neuroscience research applications. A substantial number of these have been cloned...expression, and an ER signal/leader sequence (L) for translocation of the heavy chain across the ER membrane. PCR-mediated...expression, and an ER signal/leader sequence (L) for translocation of the light chain across the ER membrane. Downstream...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...opens in a new window) UC Davis/NIH NeuroMab Publications (Link opens in a new window)...

  7. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids which use...= Substantial non-specific labeling observed Publications Ben-Simon Y, Hooper M, Narayan S, Daigle T, ...

  8. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...No. 9, pp. 741-6 Shemiakina et al. : Nature Communications, November 2012, Vol. 3 No. 1204 Wiedenmann ... Vol. 11 No. 5, pp.572-8 Yu et al. : Nature Communications, May 2014 Vol. 15 No. 5, p. 3626 Do you have...

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Controls To look up a viral vector with the Penn catalog ID: Based on low demand, production of some viral...discontinued. The following viral vectors in the indicated serotypes are no longer available as aliquots....

  10. Zinc Finger Consortium Reagents

    Type
    Collection
    ... and nonprofit laboratories for research and educational use. Links to additional pages describing these...

  13. AAV Viral Preps

    Type
    Collection
    ...Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic Neuronal Tracing...

  14. Plasmid Collections

    Type
    Collection
    ...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...

  15. Mammalian RNAi Tools

    Type
    Collection
    ...search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1...

  16. CRISPR Plasmids and Resources

    Type
    Collection
    ...fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR libraries are a powerful tool ...

  17. Recombinases AAV Preps

    Type
    Collection
    ...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  18. Chemogenetics AAV Preps

    Type
    Collection
    ...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  19. Lentiviral Prep Service

    Type
    Collection
    ... Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...Society (IZFS) - An organization that promotes and advocates for zebrafish research in the international research...

  21. Control AAV Preps

    Type
    Collection
    ...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  22. Brain Armamentarium

    Type
    Collection
    .... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru 163505-PHPeB...

  23. Lentivirus Plasmids

    Type
    Collection
    ...included to monitor expression Parijs 11619 pLB 3rd Modification of pLL3.7; Genetic elements known to prevent...

  24. Optogenetics AAV Preps

    Type
    Collection
    ...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  25. Deisseroth INTRSECT Collection

    Type
    Collection
    ...inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene...

  26. Biosensor AAV Preps

    Type
    Collection
    ...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  27. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Spinocerebellar ataxia 22 Bernard Weinstein 21238 PKC gamma CAT PRKCG HA CMV Spinocerebellar ataxia 21 Bernard Weinstein... HA CMV ALS Catherine Tomasetto 104450 pEGFPC1-hVAP-B KD/MD VAPB GFP, HA CMV ALS Catherine Tomasetto 104465...

  29. Educational Resources

    Type
    Guide
    ...Addgene's educational resources, including eBooks, science guides, videos, and protocols... Educational Resources...eBooks to videos and blog posts to protocols, our educational resources cover a wide range of topics in molecular...written and video protocols for a wide range of applications in molecular biology, plasmid cloning, and virus...

  30. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...Origin of Replication? See our Plasmids 101: Origin of Replication blog).  The replication problem Plasmids...directly inhibit replication by binding to the origin of replication and blocking replication machinery (del... of microbial plasmids: classification and identification based on replication and transfer systems and...plasmid that contains the origin of replication and the replication control machinery (Need a refresher... that inhibits replication when copy number in the cell is high, but allows replication when copy number...either indirectly inhibit replication through inhibiting translation of replication machinery proteins, or...antisense RNAs inhibit plasmid replication.   Figure 1: The replication problem. Iterons are repeated...

  31. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ... can and do consume science communication.  Formal education is education that is provided through a structured...underlying all communications efforts.  Scicomm definitions (as used by Addgene)    Science communication, or science...time engaging with science communication efforts.  Scientific communication or scientific writing is peer...to note that it is the actual communication itself, not the communication method, that determines if something...scientific or science communication. A Tweet is still considered scientific communication if the language is... engage or disengage at will.  Informal education is education that is provided, typically ad hoc, for...Series, where we’ll be talking about science communication, best practices in the field, and even getting...

  32. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...epigenome (the sum of epigenetic modifications across the genome) has been implicated in disease states, and targeting...DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of epigenetic... the catalytic domain of p300 acetyltransferase increases levels of H3K27ac histone modification at specified...Preps for Epigenetic Modification Why use epigenetic modifiers? Epigenetic modification is certainly not ...Nature Communications 8: . https://doi.org/10.1038/ncomms16026 Find plasmids from this publication at Addgene..., 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional layer of control over gene...efficient. In general, epigenetic chromatin modifications are correlated with alterations in gene expression...

  33. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...that allows category-specific, narrow searches. We defined three top level categories — catalog, educational... in a given category, and serve as a shortcut to a search scoped to that category. Catalog ID suggestions...prefer to browse and discover items in our catalog or educational resources through our main dropdown menu... of Addgene’s customer’s needs. Categories We created a categorization of our information in order to ...depositors — each with subcategories such as plasmids and viral preps for catalog, blog posts and protocols...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...sequencing data; as a result, the materials in our catalog are very well-characterized. During this time, ...

  34. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...the grant application process. When my PhD advisor first asked me to write a grant application due the ...in a previously funded grant application to see how funds were allocated. Step 3: Get feedback from your...the next round of applications. Step 5: Keep writing! Each written grant application builds upon the ... project grant application" by the NIH Still want more information on the application process? What types...advisor alone applies for these competitive grant applications; however, in some cases, your advisor may choose...writing, volunteered to take the lead on various applications throughout my graduate career, and helped receive...Proposal writing: Improves technical writing and communication skills, Elucidates the big picture and future...

  35. Plasmids 101: Protein tags

    Type
    Blog Post
    ...or DYKDDDK 1 Affinity and Purification Good for antibody-based purification; has inherent enterokinase...site GST Large Protein 26 Purification and Stability Good for purification with glutathione; protects...used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small...GKPIPNPLLGLDST   1.4 Affinity and Purification Good for antibody-based purification Combo and cleavage tags...multitude of uses including (but not limited to) purification, detection, solubilization, localization, or...fusion proteins to aid in the expression and purification of recombinant proteins. Tags for stability...be fused to recombinant proteins for one-step purification with glutathione, can also protect against proteolysis...

  36. Deep Dive: qPCR

    Type
    Blog Post
    ...single peak, indicating one amplicon and no primer dimers. (b) A shoulder peak indicating primer dimers...Method Standard Curve?  Amplification Efficacy Calculation Amplification Efficacy Assumptions ...understanding and technical proficiency with common applications - now let’s dive right in! You know qPCR is...is most commonly used to measure relative quantification of mRNA, which can be used as a proxy measure...as it solves, so understanding and using the application correctly is imperative. Picking your chemistry... at least 25 bp away from each primer, ideally located close to either the forward or the reverse primer...dimers. (c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma...

  37. Engaging with science and society at pgEd

    Type
    Blog Post
    ...variety of projects in the space of science education, communication, and policy. In this post, I hope to tell...science policy and science communication A career in science communication or policy requires broad interest...pursuing a career in science communication or policy, being able to communicate your work to a non-technical...series on careers in science communication Find all of our science communication posts Extracurricular activities... of New Initiatives for the Personal Genetics Education Project (pgEd). Advances in genetic technologies...inequalities and injustices. The Personal Genetics Education Project (pgEd), where I currently work, strives... as well as the personal, social and ethical implications of genetics. In these conversations, pgEd strives...

  38. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...switches. Stabilizing gRNA modifications  Sugar phosphate backbone modifications The recognition sequence...phosphorothioates with 2’-O-methyl modifications, proved more stability than just one modification alone (Hendel, et al...ordered as synthesis modifications when purchasing your gRNA. Pro tip: modifications are typically incorporated...stabilizing modifications listed above were developed before CRISPR-Cas9, for use in applications like siRNA...worrying about the status of your RNA. gRNAs modifications are even going beyond stability, adding color...cellular RNA molecules with post translational modifications. One of the most common of these is 2’-O-methylation...nucleolytic attack. Another stabilizing sugar modification, found at the same site, is 2’-fluoro (2’-F)...

  39. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...FAIRE-seq, and CHIP-seq), genome-wide identification and quantification of enhancer activity have remained...compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides an ...CpG methylation by fusing catalytically inactive Cas9 (dCas9) with the catalytic domain from DNA methyltransferase...  Superglue proteins with SpyTag/SpyCatcher and SnoopTag/SnoopCatcher The development of recombinant DNA...alternating the use of the SnoopTag/SnoopCatcher and SpyTag/SpyCatcher pairs. In order to improve the ease...MBPx-SpyCatcher construct. This construct is used to produce a maltose binding protein/SpyCatcher fusion...of larger fusions through SpyTag/SpyCatcher and SnoopTag/SnoopCatcher linkages. Once all of the desired...

  40. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...higher education nonprofit entities related to or affiliated with institutions of higher education nonprofit...for self-sponsorship of the I-140 application meaning that the category does not require a foreign national.... NIW requirements stress “educational background, expertise, publications, and plan for future activities...received 116,224 employment based green card applications and approved 91,023 during the first 3 quarters...for a role that is closely aligned with their education/experience, i.e. a research role, for a Ph.D. ...well-tailored to meet the needs of researchers. The categories are as follows: Cap Subject Industry H1B Academic...occupation" as requiring theoretical and practical application of a body of highly specialized knowledge in ...
Showing: 161 - 200 of 1023 results