We narrowed to 277 results for: mel

1. 3 Challenges in Plant Synthetic Biology

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   ...synthetic biology is still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...

2. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...pretty much all investigators. Our kit makes it extremely simple to design TALENs. It only takes two to ...

3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...networks. “Developmental bioelectricity is an extremely exciting emerging field, with many implications...

4. RNA Extraction Without A Kit

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   ...within tissues before cell lysis. These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen...

5. Transferable Skills Guide: Public Speaking

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   ...nervous and freeze, or forget a point, or talk extremely quickly. You realize, much too late, that you ...

6. Giving gRNAs a Facelift - Synthetic and Beyond

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   ....2021.02.007 Moroz-Omori, E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally...

7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...antibodies, nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are ...

8. The Future of Research Symposium Boston 2015

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   ...graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor, Director of ...

9. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...anywhere else, but for synthetic circuits, they are extremely orthogonal. It’s a different perspective and you...

10. Hot Plasmids - October 2020

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    ...targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid...

11. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine base editors...

12. Early Career Researcher Toolbox: Finding Relevant Papers

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    ...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications...

13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ... ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying and assessing ncRNA...

14. Making CRISPR Plasmids Using Fragmid

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    ...   Figure 1: (Top) Schematic overview and timeline of the Golden Gate (GG) cloning approach. Individual...

15. Antibodies 101: Stripping and Reprobing Western Blots

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    ...to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a fume hood. ...

16. Plasmids 101: Modular Cloning

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    ...combinations. Even with current technologies, it can be extremely hard to predict how a single TU, or even an individual...

17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...epitopes are available; this type of ELISA is extremely specific and sensitive to the target protein due...

18. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ... Melina Fan...

19. Hot Plasmids and Viral Preps - March 2021

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    ...status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed a set of ...

20. Hot Plasmids and Viral Preps - January 2021

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    ...24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox...

21. Viral Vectors 101: Chemogenetics

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    ...Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D,...

22. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...M.K. Montgomery, S.A. Kostas, S.E. Driver, C.C. Mello. Potent and specific genetic interference by double-stranded...

23. Fluorescent Proteins 101: Introduction to FRET

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    ... The first genetically encoded FRET biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure...

24. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ..., X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae ...

25. Tips for Technical Support Calls

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    ...response, however, consider following up. Ask for a timeline if you are not given one. Though an initial response...

26. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ...your action item list and set some approximate timelines. Be sure that you keep your commitments, and if...

27. Academic vs. Industry Postdocs

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    ...afford to spend too much time working on something extremely risky or exploratory. Getting a job afterwards...

28. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...epigenetic modifications, we can start to build a timeline of events that surround changes in gene expression...

29. Magnetic Control of Proteins: More than a Dream

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    ...Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification...

30. Pooled Library Amplifications

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    ...Re-Amplification Protocol (PDF, 71 KB) Barcode Watermelon Library 5,000,000 30 bp At least 200 ng...

31. PCR Applications for Copy Number Variation Assays

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    ...not need to be run in triplicates. ddPCR is an extremely accurate application, even in areas of the genome...

32. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...your favor. For starters, Golden Gate cloning is extremely efficient due to the use of the versatile type...

33. Plasmids 101: Protein Expression

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    ... inducible promoters. Inducible promoters are extremely useful if a desired protein product is toxic to...

34. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...line. Knock downs in Drosophila cell lines are extremely effective. Their Snapdragon tool helps you select...

35. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016):1777-85. PubMed...

36. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...Replacement by Compound Autosomes in Drosophila melanogaster. Genetics 74, 461–475 (1973). PubMed. More ...

37. Plasmids 101: Multicistronic Vectors

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    ... Melina Fan...

38. Simplify Cloning with in vivo Assembly

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    ...First design the template binding region, with a melting temperature (Tm) of around 60 °C for all primers...

39. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...labs and institutions. For example, search for “Melina Fan” and you’ll be able to go directly to a page...

40. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley, W. K., & Brooks, G. F. (1986). ...