Addgene

Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Type

Blog Post

...Central PMCID: PMC4176945.
4. Kleinstiver, Benjamin P., et al. "High-fidelity CRISPR–Cas9 nucleases with...

CRISPR in the Clinic

Type

Blog Post

...(2022). doi:10.1186/s12943-022-01559-z
Zarghamian P, Klermund J, Cathomen T. Clinical genome editing to...

Synthetic promoter AAVs for cell-type specific expression in retinal cells

Type

Blog Post

...Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, ...

Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

...PubMed Central PMCID: PMC4243060.
4. Messina, Jane P., et al. "Mapping global environmental suitability...

The Materials Science of Optogenetics Experiments

Type

Blog Post

...Thank you to our guest blogger Derek Simon!

Derek P. Simon received his PhD in Cellular and Molecular ...

CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Type

Blog Post

...020-0771-6
Mayo-Muñoz D, He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based...

PCR Applications for Copy Number Variation Assays

Type

Blog Post

...Hyg. 2008 Dec;79(6):887-92. PMID: 19052298.
Muhamad P, Chaijaroenkul W, Congpuong K, Na-Bangchang K. SYBR...

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Type

Blog Post

...Concepcion CP, Bonetti C, Vidigal JA, Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura...

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

...Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., … Forster, A. C. (2018). Engineering a palette of...

5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

... 291-306. PubMed PMID: 10634178.
8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination...

Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

... L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from ...

Antibodies 101: Producing Recombinant Antibodies

Type

Blog Post

...Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor supports...

Getting to Know Expansion Microscopy

Type

Blog Post

...s41592-018-0238-1 
Klimas A, Gallagher BR, Wijesekara P, et al (2023) Magnify is a universal molecular anchoring...

Course-Based Undergraduate Research Experiences (CUREs)

Type

Blog Post

.... 38(5):317-323.
Knutson, K., Smith, J., Nichols, P., Wallert, M., Provost, J. 2010b. Bringing the excitement...

CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

...10.7554/eLife.04766
Moreno-Mateos, M. A., Fernandez, J. P., Rouet, R., Vejnar, C. E., et al. CRISPR-Cpf1 mediates...

New CRISPR Tools: Cas7-11 and PASTE

Type

Blog Post

...Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan...

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

...1505. https://doi.org/10.1126/science.abd0826
Tong P, Gautam A, Windsor I, Travers M, Chen Y, Garcia N,...

Viral Production at Addgene

Type

Blog Post

... element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization...

Plasmids 101: Choosing an Antibiotic Resistance Gene

Type

Blog Post

...., Emrich, C. A., Jeans, C., Faulkner, M., Riggs, P., & Berkmen, M. (2012). SHuffle, a novel Escherichia...

Viral Vectors 101: The Retroviral Lifecycle

Type

Blog Post

... 2304918. PubMed Central PMCID: PMC53514
Rowe, W. P., Hartley, J. W., Lander, M. R., Pugh, W. E., & Teich...

Plasmids 101: Shuttle Vectors

Type

Blog Post

... Emily P. Bentley...

Tips for arabidopsis transformation

Type

Blog Post

...transformation’, Plant Methods. BioMed Central, 2(1), p. 19. doi: 10.1186/1746-4811-2-19. PubMed PMID: 17087829...

AAVs in Retinal Gene Therapy

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Blog Post

... Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046.
6. The Forever Fix: Gene...

Plasmids 101: Gateway Cloning

Type

Blog Post

... design. They have modified versions of the attB, P, L and R sites that recombine very specifically and...

The Challenges of Cell Culture

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Blog Post

...315-319. PubMed PMID: 12001993.
4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins. "...

CRISPR Antimicrobials

Type

Blog Post

...PMC4466736.
5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral...

Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...this publication at Addgene.

Kleinstiver, Benjamin P., et al. "Genome-wide specificities of CRISPR-Cas ...

What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Type

Blog Post

...to Phenotype thus making progress on the NSF “G to P grand challenge.”
Creating a productive, new scientific...

Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...aid-ajmg6>3.0.co;2-o
Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY (2013) An AT-Hook Domain in...

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

... 86–90 (2018).
Farhadi, A., Ho, G. H., Sawyer, D. P., Bourdeau, R. W. & Shapiro, M. G. Ultrasound imaging...

CRISPR 101: Validating Your Genome Edit

Type

Blog Post

...Shkumatava A, Teboul L, Kent J, Joly J-S, Concordet J-P. Evaluation of off-target and on-target scoring algorithms...

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...Christie, K. A., Whittaker, M. N. & Kleinstiver, B. P. Unconstrained genome targeting with near-PAMless ...

Viral Vectors 101: Pseudotyping

Type

Blog Post

...10.1093/hmg/10.19.2109
Sandrin V, Boson B, Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L ...

Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

... Emily P. Bentley...

Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...Central PMCID: PMC3456965.
11. Zhang B., Metharom P., Jullie H., Ellem K.A., Cleghorn G., West M.J., Wei...

Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...(is that pun worn out yet?) your research.

Derek P. Simon received his PhD in Cellular and Molecular ...

Five Popular Model Organisms

Type

Blog Post

...PubMed Central PMCID: PMC5349099.
Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular...

An Integrin Antibody Toolkit from IPI

Type

Blog Post

...reveal αV subunit pairing preferences with β subunits (p. 2024.01.26.577394). bioRxiv. https://doi.org/10.1101...

SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Type

Blog Post

...doi.org/10.1038/s41596-019-0210-2
Lucia, C., Federico, P.-B., & Alejandra, G. C. (2020). An ultrasensitive,...

Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...S13–S13 . https://doi.org/10.1038/ni.3608 
Monnier P, Vigouroux R, Tassew N (2013) In Vivo Applications...