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We narrowed to 400 results for: pho;

Showing: 201 - 250 of 400 results

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...for clearer characterization of the location, morphology, and circuit mapping of a targeted neuronal population...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...commonly used promoters include the phosphate and copper inducible PHO5 and CUP1 promoters respectively. ...

  8. Pooled Library Amplifications

    Type
    Blog Post
    ...Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ...

  10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...your samples and maintaining cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker...reagents - cross-linkers better preserve sample morphology, while organic solvents can reveal otherwise ...

  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...basic science research (neuronal tracing and morphologic studies), as well as translational applications...capsids are useful tools for neuronal tracing and morphology studies when used as part of a two part fluorescent...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...including a lot of future Addgenies as shown in the photo below.  Kids and adults alike enjoyed some friendly...of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget about...

  17. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...sample lysates will also contain proteases and phosphatases that may degrade the target protein. To prevent...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and ...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs...in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman...

  21. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer... How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction...

  22. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic...

  23. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...affinity reagents to study the difference in cell morphology changes induced by integrins binding the RGD ...

  24. Evolution of Lab Techniques

    Type
    Blog Post
    ... at Harvard University. Just as computers, cell phones, and cars become more technologically advanced ...sequences among DNA fragments separated by gel electrophoresis." Journal of molecular biology98.3 (1975): ...

  25. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...kinase is to express them with an appropriate phosphatase, a technique originally pioneered by Markus Seeliger...(Seeliger et. al. 2005). In this kit, Lambda phosphatase (Plasmid 79748) enhances bacterial expression...

  26. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    .... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  28. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...(a thymine in this case) is only bound by the phosphate backbone. These PAM bases must be present for .... Instead, both lobes bind to the DNA and RNA phosphate backbones, allowing Cas9 to verify the structure...

  29. Technical Design of a Western Blot

    Type
    Blog Post
    ...horseradish peroxidase protein (HRP) and alkaline phosphates. HRPs are usually preferred because of their ...conjugating your antibodies yourself. Alkaline phosphates have a linear reaction rate and are compatible...

  30. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...recommendations. Tris-buffered saline (TBS) or phosphate-buffered saline (PBS) are the most commonly used...

  31. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ... creative scientists delivered some spectacular photos once again this year! All winners receive an Addgene...

  34. Deck the Lab 2022!

    Type
    Blog Post
    ... contest, you need only decorate your lab, take photos, and then share them on Twitter or Instagram with...

  35. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ... down and read through each one, plug in some headphones to learn more about the latest and greatest plasmids...

  38. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor from Avena sativa, light-mediated genome editing...

  42. Let There Be LITE Plasmids

    Type
    Blog Post
    ...light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate...
Showing: 201 - 250 of 400 results