We narrowed to 208 results for: pme i

1. Antibodies 101: Introduction to Antibodies

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   Blog Post

   ...that antibodies can be used for a lot of purposes! I hope you appreciate how many types of antibodies are...reagent sharing platform for antibodies, the Developmental Studies Hybridoma Bank Learn more about nanobodies...

2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   Blog Post

   ...crRNAs that bind to the template strand. Therefore, I suggest that picking a crRNA complementary to the ...manipulation of mammalian neural stem cells. Development 144: 635-648. PubMed PMID: 28096221. PubMed Central...

3. Optogenetics + CRISPR, Using Light to Control Genome Editing

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   Blog Post

   .../doi.org/10.1038/nchembio.1753 Richter F, Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier...Dalvie NS, Panda A, Fleming HE, Bhatia SN (2016) Development of Light-Activated CRISPR Using Guide RNAs with...

4. Technical Design of a Western Blot

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   Blog Post

   ...., Podrebarac, D. M., Greenough, W. T., & Weiler, I. J. (2008). The use of total protein stains as loading...that each type of transfer requires different equipment. Wet transfers can be done in an insert in your...

5. Gamma-Retroviral Vector Guide

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   Guide

   ....2022.12.006 PMID: 36620071 Mirow, M., Schwarze, L. I., Fehse, B., & Riecken, K. (2021). Efficient pseudotyping...lead to activation of the oncogene and possible development of cancer. Pseudotyping Tropism dictates which...manufacturing. Molecular Therapy — Methods & Clinical Development , 28 , 116–128. https://doi.org/10.1016/j.omtm...

6. Neurodegeneration Plasmid Collection

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   Collection

   ...dynactin1-811 DCTN1 mKate2 hsp70 ALS Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS Caren Norden...CMV, P10 Huntington's Cheryl Arrowsmith 112205 pBS-I-Sce1-eno2:Tau-IRES-egfp MAPT GFP, eno2 eno2 Parkinson's...Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia Richard...

7. Adenovirus Guide

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   Guide

   ...Watanabe, M., Nishikawaji, Y., Kawakami, H., & Kosai, K. I. (2021). Adenovirus biology, recombinant adenovirus...verified, and linearized with the restriction enzyme PmeI . This construct is then transformed into AdEasier...cassette in one single viral particle. Vaccine Development rAdV vectors are highly immunogenic because they...

8. Adeno-associated virus (AAV) Guide

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   Guide

   ...PMID: 12663794 (Link opens in a new window) Kwon, I., & Schaffer, D. V. (2008). Designer gene delivery...be limited to small transgenes, enabling the development of further AAV applications. Read our blog post...opens in a new window) Merten, O. W. (2024). Development of stable packaging and producer cell lines for...