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We narrowed to 969 results for: Abo;

Showing: 81 - 100 of 969 results
  1. Chemogenetics AAV Preps

    Type
    Collection
    ... Chemogenetics Guide pages for more information about this field. Receptor hM3D(Gq) DREADD rM3D(Gs) DREADD...
  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ...optimized for both safety and efficiency. Learn more about the different types of viral vectors and find viral...
  3. Microbiology Resources

    Type
    Collection
    ...individual plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor...
  4. Worm Expression Resources

    Type
    Collection
    ... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...
  5. TALEN Guide

    Type
    Collection
    ...researchers who are learning to wield them. Learn more about Addgene’s TAL effector and TALEN kits here . References...
  6. Lentiviral Prep Service

    Type
    Collection
    ...are reported with each shipment. For more details about our viral vector packaging service, visit our Viral...
  7. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins ...
  8. Open Enzyme Collection

    Type
    Collection
    ...engineering and directed evolution. More information about the collection can be obtained at the Open Bioeconomy...
  9. Brain Armamentarium

    Type
    Collection
    ...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...
  10. CRISPR Plasmids - Tagging

    Type
    Collection
    .... Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to...
  11. Optogenetics AAV Preps

    Type
    Collection
    ...and Optogenetics Guide page for more information about this field. Use the table and filters below to find...
  12. Luciferase Plasmid Collection

    Type
    Collection
    ... vivo imaging or creating biosensors. Read more about BRET on our blog . Nano-Lanterns: Fluorescent proteins...
  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...
  14. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Link opens in a new window) together with the laboratory of NeuroMab Director, Dr. James Trimmer, have...nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain...
  15. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be followed in addition... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves... materials. Video Watch the video to learn more about safety in the lab. Equipment Lab coat Close-toed... eat, drink, chew gum, or apply makeup in the laboratory. Before you start your experiment, make sure ...Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some times...not be worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names...names and contact information of the laboratory manager should be clearly visible in the room and on the ...
  16. Pipetting Protocol

    Type
    Protocol
    ...Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle...liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures the accuracy...100 from top to bottom (as shown in the picture above), while 650 µL would read as 065. P200: The first...100 from top to bottom (as shown in the picture above), while 95 µL would read as 095. P20: The first ...10 µL would read as 100 (as shown in the picture above), while 2.2 µL would read as 022. P2: The first ...1 µL would read as 100 (as shown in the picture above), while 0.5 µL would read as 050. Each volume display...
  17. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...cool down to about 50 °C (about when you can comfortably keep your hand on the flask), about 5 mins. Optional...concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr... of the tip of the pipette into the buffer just above the well. Very slowly and steadily, push the sample... of the way down the gel. A typical run time is about 1-1.5 hours, depending on the gel concentration ...
  18. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing... not already been done. We typically start with about 250 mL of supernatant and add 250 µL of 100X protease... sample among multiple columns. For information about the total binding capacity for your columns, refer...setting. If the concentration of the pooled sample is above 1.0 mg/mL proceed to Option 1 with a buffer exchange...based on the concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column...
Showing: 81 - 100 of 969 results