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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 430 results for: ERG

Showing: 81 - 100 of 430 results

  1. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion...

  2. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...light-sensitive proteins that absorb photons for energy conversion or the induction of intra- or intercellular...shorter than 5 kb (Hirsch et al., 2016; Hudry and Vandenberghe, 2019). How to achieve strong opsin expression...PubMed PMID: 26135556. Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous...Juarez, Barbara, and Ming-Hu Han. "Diversity of dopaminergic neural circuits in response to drug exposure...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...emitting a photon with slightly less energy due to non-radiative energy losses. This difference in emission...ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to ...excitation and emission profiles to enable effective energy transfer, which is key to observing interactions...

  4. Synthetic Biology - Overview

    Type
    Collection
    ... and ethical synthetic biology JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering...

  6. Distribution to Industry

    Type
    Collection
    ...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...

  8. Antibody Plasmid Collection

    Type
    Collection
    ...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...

  9. Microbiology Resources

    Type
    Collection
    ...Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions...

  10. Worm Expression Resources

    Type
    Collection
    ...promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-...

  11. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ... its confines. The beauty of serendipity often emerges at the intersections of disciplines. Cast your ... capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling...serendipity. However, the best discoveries often emerge when we allow ourselves the freedom to explore ...

  13. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...sequences. Repeating sequences within a plasmid can undergo intramolecular DNA recombination. This naturally...two repeating elements love each other very much undergo recombination, two smaller plasmids are formed....To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers find that...

  14. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... An undergraduate can mentor a high school student, a graduate student can mentor an undergraduate, a ... up on plans. Make a commitment to put time and energy into your mentoring relationships. You will only...

  15. TALEN Guide

    Type
    Collection
    ...Jens Boch at the Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published...

  16. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...non-governmental groups with seeking “public views on emerging IVF techniques designed to prevent the transmission... a controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR/Cas9 –...in the software industry to inform her work on emerging issues in novel technologies, including self-driving...

  17. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Instead, R body monomers respond to pH changes by undergoing local conformational changes. These tiny movements...decades, and they were known to be resistant to detergents and degradation under a variety of conditions...conditions. More speculatively, R bodies might endow divergent organisms with new functions. For inspiration,...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...ll provide information about commonly used and emerging capsids to help you decide which ones may be suitable... of published descriptions of commonly used or emerging capsids. Selected capsids are further highlighted... regional bias towards the cortex and thalamus emerges. AAV-PHP.eC effectively transduces neurons and ... Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg, M. C., Lagor, W. R., Heaney, J. D., Chan, Y. ... Sakopoulos, S., Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith...

  19. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered...distinct effector domains that work together synergistically, modeled after natural transcription activation... J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015...

  20. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...cargoes, genetically engineered neurotropic viruses emerged as powerful tools for visualizing neural connectivity...monosynaptic tracing to identify inputs to two major dopaminergic cell populations in two specific brain regions...617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, Lepier A, Götz M, Berninger...
Showing: 81 - 100 of 430 results