...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch ...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab...

...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more. Viral...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other Cre-dependent color ...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...BGHpA (Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium ...

...non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore (the acceptor) by means of ...the two fluorophores used are in close vicinity. Thus, the distance between the two fluorophores and their... of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore colours... filters chosen for the donor fluorescence and the acceptor fluorescence. Additionally, this method could... the fluorescence decay time of the donor. When FRET occurs between the pairs, donor fluorescence is quenched...factors, such as pH or autofluorescence background, can change the fluorescence decay time and have to...course talking about the now well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique...

...for autofluorescence extraction Cells produce a low level of fluorescence called autofluorescence (you...to identify a fluorophore, spectral flow cytometers use all detectors for all fluorophores. This allows...tool called “autofluorescence extraction” which allows the user to use the autofluorescence in a sample...sample as a fluorescent marker. Given that the level of autofluorescence across various tissue types varies...you wish to use the autofluorescence extraction feature. Although autofluorescence extraction is an optional...samples stained with a single fluorophore. They’re required for every fluorophore you use in your panel, whether... the spectral signature of a fluorophore itself from the autofluorescence of the beads or cells. Overall...

...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology... Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus ...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent... You may also like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR...Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...you are looking for, such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter...

... blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent protein...area with lots of bright new plasmids is our Fluorescent Biosensors Collection — scientists are always...measure! Let’s take a look at some of the hottest fluorescent biosensors new to Addgene this year. Feel the...Hiroshi Hibino and colleagues devised a “Modular fluorescence unit fused with TRansmembrane region-to-IntrAcellular...region-to-IntrAcellular loop linkers” (MTRIA for short), where a fluorescent protein is inserted into one of the loop regions...  Find MTRIA sensors here!   Ino D, et al. A fluorescent sensor for real-time measurement of extracellular...co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence localizes to the plasma membrane only when the...

...More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins Control...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones...historically been difficult, as techniques like fluorescent in situ hybridization (FISH) and chromosome capture...be used in vivo. Some researchers have used fluorescently tagged DNA-binding proteins to label certain...chromosome. This image represents the composite of fluorescent measurements from red, blue, and green channels...targeting capacity. By using a gRNA to target fluorescently labeled dCas9 to a given genomic locus, you ... thereby decreasing the amount of background fluorescence from unbound GFP-dCas9. With these improvements...

...years — a green fluorescent protein, a red fluorescent protein, a fancy new green fluorescent protein, some...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the...purification tags, another version with that same red fluorescent protein somebody made because they forgot it ...of chemical dyes provide many advantages over fluorescent proteins, opening up new applications.   ...strategies. The green structure represents a fluorophore or other useful ligand, gray structure is the...biarsenical dyes such as FlAsH (green, based on fluorescein) or ReAsH (red, based on resorufin). SNAP-tag...dihydrofolate reductase (eDHFR) could be labeled with fluorescent derivatives of its substrate trimpethoprim (TMP-tag...

...than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent proteins... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ...good reason. We all know scientists have used fluorescent proteins to observe what’s happening inside cells... of decades. Green is the classic color, but fluorescent proteins are available in a variety of hues. ...unimpeded. The challenge then was to develop fluorescent proteins that would fall within that near-infrared... from jellyfishes and corals, but all of them fluoresce outside the transparency window of mammalian tissues... Verkhusha said. “We developed near-infrared fluorescent proteins within this transparency window, so ...

... conjugated to a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody...cases, fluorophore-conjugated antibodies may exist, but only with a limited selection of fluorophores. If... tag but not a fluorophore. In a separate step, cells are incubated with a fluorophore-conjugated streptavidin...wide range of fluorophores, allowing for great flexibility when it comes to fluorophore selection for ...common method is a cell surface label, where fluorophore-conjugated antibodies directly bind to epitopes...employ beyond simply incubating cells with single-fluorophore-conjugated antibodies. These methods can include...interaction (Figure 1a). This interaction brings the fluorophore in proximity to the target marker, allowing for...

...overcome the issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...from the fluorophore is pH-dependent. Signal is simultaneously acquired from the the fluorescent and luminescent...mimetic and induces fluorescence in the presence of DHFBI, a small molecule fluorophore. When fused to an...FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net influx into the nucleus, where the functional Cas9 molecule...visualization of single molecules via targeted fluorescent molecules. The Vale lab and the Weissman lab ...protein of interest are recognized and thus fluorescently labeled by scFv antibodies fused to sfGFP. This... This system amplifies the intensity of fluorescence signal and enables tracking of single molecules within...

...challenging due to low fluorescence intensity, caused partially by fluorophore photoinstability, as well... by immunostaining. Although the fluorophores used are fluorescently distinct, their protein sequences...cells. The Brainbow-1.0 construct contains three fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (...recombination. Brainbow-1.1 scales up the system to four fluorescent proteins, with recombination events specified...but the principles remain the same. The first fluorescent protein in the construct, OFP (orange), is expressed...two tandem invertible segments, each with two fluorescent protein coding sequences in opposite orientations...expressed; once Cre is removed from the system, the fluorescent protein whose coding sequence is closest to the...

...a magnetoresponsive fluorescent protein. Controlling an optogenetic or fluorescent tool with magnets would... do fluorescent proteins respond to magnetic fields?” and “What are magnetoresponsive fluorescent proteins...set out to do just that — by developing a new fluorescent protein tool that responds strongly to magnetic...Motivated by curiosity Although a wide range of fluorescent proteins and optogenetic tools exist, humans ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka...EGFP — showed a slight but detectable change in fluorescence when a small magnet was passed over the sample...sample (Frank Hayward et al., 2024). The fluorescence intensity decreased whenever the magnet approached...

...like chromoproteins, fluorescent proteins, and luminescent proteins. While fluorescent proteins and luminescence...from fluorescent proteins? Before going into the difference between chromoproteins and fluorescent proteins...the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to blue ...light, whereas chromoproteins do not fluoresce at all or fluoresce at very low levels. Chromoproteins in...detection. Unlike fluorescence or luminescence, which require UV lamps, fluorometers, or luminometers,...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful... chromoproteins are actually a subset of the fluorescent protein family. What sets them apart is the way...

...with rhodopsin quenching the added fluor for this two-part fluorescent system, and rhodopsin still functioning... elements: a voltage-sensing component and a fluorescent output. The GEVI family of gadgets includes Ace...Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism of their voltage sensing...VSD-based sensors consist of a VSD fused to a single fluorescent protein (FP). This class of GEVI was the earliest...of red-shifted indicators, and brightness of fluorescence all have room for improvement. These sensors...rhodopsin functions as both the voltage detector and fluorescent signaler. The pro of this system is the kinetic...were initially evolved for ion transport, not fluorescence, they are not nearly as bright as the other ...

... Since fluorescent proteins (FP) require external light activation, you can’t use fluorescence to monitor...detection is difficult using fluorescent imaging due to autofluorescence and a high signal-to-noise ratio... Learn about Photoactivatable Fluorescent Proteins Check Out Fluorescent Protein Timers Use Rosella to...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein...Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over...experimental success can be affected by both autofluorescence and photobleaching. Researchers have long ...luciferase transfers energy to the FP, resulting in a fluorescent signal. Since their first publication in 2012...

...intrinsically fluorescent and can be detected with highly epitope-specific, fluorescently-labeled antibodies...usually provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan... and David W. Piston. "Fluorescent protein tracking and detection: fluorescent protein structure and color...tracers Neuronal tracers such as epitope tags and fluorescent proteins (FP) have also been important in mapping...tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene...compared to epitopes tags, FPs are intrinsically fluorescent and can therefore be used in live imaging experiments...tracer With the limitations of epitope tags and fluorescent proteins, it has been crucial to develop new ...

...up new fluorescent markers before deciding to use them, using a tool like BioLegend’s Fluorophore Guide...the process of correcting spillover from one fluorescent channel to another. When you label your samples...samples with multiple antibodies, the fluorescent probes on the antibodies may have similar emission spectra...spectra, meaning they will emit fluorescent light at similar wavelengths (See this handy emission spectra...spectra). Your cytometer will therefore record the fluorescence for both at similar levels, meaning that your...desired cell population. By compensating between fluorescent channels, you can correct for this spillover ...your flow panel, you will need to know both the fluorescent markers you intend to use and your cytometer’...