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Showing: 81 - 100 of 1101 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc...plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type Promoter Description PI 60226...that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top ...emission of photons. The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching ...depending on the application. Firefly luciferase is considerably brighter than Renilla luciferase, but is more...systems. Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase...level of expression. Scientists have developed a wide range of luciferase-based plasmid systems for many...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP and puromycin... the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous ... . Use these tools to help identify guide sequences that minimize identical genomic matches or near-matches...CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present a video reprint from the Journal...oligos for each guide and its associated reverse complement including additional nucleotides for cloning ...Add “CACC” before the 20-mer guide sequence and “AAAC” before the guide’s reverse complement for cloning...Journal of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs...

  3. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population...Caltech Systemic Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are ...

  4. Chemogenetics Plasmids

    Type
    Collection
    ... AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Plasmid Collection You may also like... Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics... Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled protein... your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search...

  5. FlyCRISPR

    Type
    Collection
    ...Individual plasmids can be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9 : A codon-optimized ...Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...site sequence can be added by inserting an oligonucleotide linker into BsaI digested vector. Cloning targeting...additional information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input sequence ...Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... ) VPR ( Addgene #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed...utilizing one of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This process... shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski...in the context of the developing mouse retina. To aide in their studies of the regulatory networks involved...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to ...

  9. Mammalian RNAi Tools

    Type
    Collection
    ...components, like argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources...

  10. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional...protein with many available isoforms and mutants provides a shared experience for the research community...

  11. AAV for Neuronal Tracing

    Type
    Collection
    ...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell population of...deletion-mutant rabies, the essential G protein is provided to the starter cell in trans , via delivery by...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top ... encoding scFv fragments that bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP...antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following...

  13. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector...Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role...MCP tagged plasmids for visualization of proteins inside cells. Depositor...ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated ...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...are useful for labeling secreted proteins with disulfide bridges such as antibodies. MCP-tag is a mutant...

  14. Chemogenetics AAV Preps

    Type
    Collection
    ...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement...See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information about this field. Receptor... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

  15. Qi Lab CRISPR Page

    Type
    Collection
    ...Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically...Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts... hort P alindromic R epeats) pathway, as an RNA-guided DNA binding platform, to repress expression of ... Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair complementary... are described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene ...23452860 . PMC3664290 . CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert...

  16. AAV Packaged on Request

    Type
    Collection
    ... plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

  17. Brain Initiative Collection

    Type
    Collection
    ...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...of the brain generated through this effort will provide novel ways to treat, cure, and even prevent brain... the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative grants...jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A. Mark Histed 179459-AAV1 pAAV-EF1a-DIO-JEDI...available at Addgene. AAV Viral Service . Addgene provides a viral service to help facilitate scientific ...

  18. Genetic Code Expansion

    Type
    Collection
    ...contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI...to enhance non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites...modified codons for unnatural amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past...from the tRNA is incorporated into the growing peptide chain. To expand the genetic code, modified tRNAs... the genetic code, there are several things to consider. If you are working off of a previously established...

  19. Validated gRNA Sequences

    Type
    Collection
    ...Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...in the associated article (listed below by PubMed ID). These factors include: Does a given gRNA sequence...gRNA Target Sequences You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...in Addgene's database as well as from sequences provided to us by users (see below for more details). Keep... Keep in mind that additional factors must be considered before using a validated gRNA sequence in your...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    .... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top ...rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater fish...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ...CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung...scientists in Latin America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A...zebrafish-specific gene expression database that provides information about when genes are active in developing...
Showing: 81 - 100 of 1101 results