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We narrowed to 469 results for: LARGE

Showing: 81 - 100 of 469 results

  2. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...fluorescence. It is useful for activity imaging of large populations of densely labeled neurons because background...fluorescence.  It is useful for activity imaging of large populations of densely-labeled neurons because background...accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR...

  4. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...toolbox. However, both of these proteins are quite large — comprising approximately 1000–1400 amino acids... cell types, it has a limited capacity to carry large DNA cargos. As a result, the search for smaller,...capacity, which makes it difficult to accommodate the large coding sequence of commonly used nucleases like ...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...and to verify the quality of the prep. This was a large factor in Addgene’s decision to launch our viral...category, available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors...were distributed in 2023 alone. You’ll also find a large number of requests for vectors in the “controls”...

  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...solution that fits the most labs, our team conducted a large study which focused on the adoption of ELNs in labs...talking about ELN costs and limited budgets, “a large percentage of survey respondents indicated that ...support that. In addition, if you are working for a large institution or company, site licenses might be available...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...to efficiently deliver large repair templates for applications that require large gene fusions. The current...Matthew H. Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...guides directed towards that gene as there can be a large difference in guide potency. Thus, in cases where...pathways and genetic interactions. Additionally, large scale CRISPR-based screens can be used to gain insight...target effects are generally not regarded as being a large problem for Cas9 activators. This is believed to...makes it much cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based...

  9. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...more efficiently amplified than larger constructs, but plasmids as large as ~6 kb can be amplified fairly... This is particularly relevant in cases where a large plasmid with multiple functional regions is used... if you want to introduce a point mutation in a large construct. Particularly, if a pair of unique restriction...visible in a digest of the unmodified plasmid into a larger band (not shown). As a footnote; a similar approach...site, can identify these duplications (slightly larger bands sizes relative to the original template)....

  13. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...

  14. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...optimized by testing various linker configurations in a large-scale functional screen in bacteria. Sensor variants...quite a robust way to make a FRET sensor with a large dynamic range,” he says. Merkx recently applied ...

  15. CRISPR Guide Update

    Type
    Blog Post
    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...absence of deletion, the “deletion band” is often too large to efficiently amplify. Typically use primers at...identified with the desired deletion and move to larger plate or flask for growth. Validation of Biallelic...

  18. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...

  19. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...

  20. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...Musunuru: We have been using the kit to target a large number of genes involved in cardiovascular and metabolic...power here is that you could potentially sequence a large number of individuals and find several mutations...
Showing: 81 - 100 of 469 results