... Many thanks to our guest blogger David Mellor! David Mellor leads the policy and incentive programs ...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...Before coming to the Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement...at https://orcid.org/0000-0002-3125-5888 and @EvoMellor.  ...

...wet lab wasn’t the right path for me. I began exploring alternative career trajectories within the industry...self-motivated and excelled when given the freedom to explore projects autonomously. Failing to recognize and...frustration, micromanagement, and disengagement. By tailoring my leadership approach to each person, I was able... a bit to my mentors, as they encouraged me to explore small-scale opportunities where I could make mistakes...role. My advice is: Give yourself permission to explore, reflect on your priorities, and embrace these ...

...guest blogger Brittany L. Uhlorn from the University of Arizona. Brittany L. Uhlorn is a fifth year Cancer...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps... is their background relative to your research? Tailor your language to your audience so that they can...This will give you a chance to determine how to tailor your presentation so that it can be understood ...

...with spectral flow—you can run a three color panel or a 40 color panel! When preparing a spectral flow ...experiment. For very large panels (upwards of 30-40 colors), spectral flow cytometry might be the better choice... Figure 3: Similarity index across 12 different colors on a three laser spectral flow cytometer. ...from a batch that is not the same as your single color control. Reference controls: Beads or cells? Compensation...

...until new inflorescences begin to grow. This will also increase the number of inflorescences overall, ...mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation method. The general ...transformation, you can cut off the primary inflorescence (the flowering portion of the plant) near the...transformed Arabidopsis thaliana seedlings following floral dip transformation’, Plant Methods. BioMed Central...

... pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression...

...Knockout Human Garnett 3rd 2 37,722 Green monkey (Chlorocebus sabaeus) sgRNA library 178284 Knockout Green ...

...focus and exploration Researchers face a fundamental conflict. Science is about exploration, discovering...will be more competitive for fellowships. If you explore your system more, you will have a better idea of...question or are you simply describing a phenomenon? Exploration can be important, but it is equally important...foods with minimal processing, and low in empty calories will make you feel better and help maintain your...

...emission spectrum. New fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov...some types of corals and anemones are vibrantly colored. He hypothesized that GFP-like proteins could be...Discosoma sp. and Katushka/mKate from Entacmaea quadricolor. With his discoveries, another part of the fluorescent... studies in Tsien lab, covered a portion of the color spectrum that GFP and its derivatives did not previously...

... and treatment of cancer have been extensively explored. Biologists have attempted to study cancer from...ve been studying it and, now, researchers are exploring ways to bolster and modulate our immune defenses...bowel disease is associated with the onset of colorectal cancer. Strategic immune evasion mechanisms -...efficient outcomes, collaborative therapies must be explored. When attacking cancer, multiple immunotherapy...and cancer immunotherapy, and is interested in exploring new avenues in healthcare.   References 1. Vinay...

...stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the...wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and relies ...algae host for reproduction. Snail parasite Leucochloridium is a parasitic worm that infects a snail’s ... the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723...

...and boost your productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding your...For binders of protocols and data, consider using color coded binders and tabs (for different experiments...notebooks and go wild with sticky notes and labels. Color code your Post-its and write brief descriptions ... the notebooks, so that you can easily spot the colors and text. Tip 2: Use electronic to-do lists to ...

...imaging cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012... monitor protein-protein interactions through multicolor labeling and FRET. Specialty constructs for biosensing...e.g., EGFP and YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable...at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer a comprehensive...

...The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University, and the Karolinska...protein CFP484. Collaborating with scientists from Florida State University, they used a mutagenesis approach... max emission at 509 nm), making it useful in multicolor labeling particularly with fluorophores that ...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology...

...salt, Sigma Aldrich P4543-10G Polyethylenimine hydrochloride, M.W. 40000 (PEI-MAX), Linear, Transfection ...7.0 with 10 N sodium hydroxide (NaOH) or 5 N hydrochloric acid (HCl). Note: Adjust the pH slowly, adding...

...another gene controlling eye color) and yellow (a gene affecting the body color of the adult fly). This is...such marker is the white gene, which restores eye color to fly lines where the endogenous white gene is ...anywhere from light yellow to red. The variation in color depends on how transcriptionally active the genomic...

... a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose...galactose and an insoluble blue pigment (4-chloro-3-brom-indigo). So far, so good, but how does this help us...β-galactosidase, and remain the lovely whitish-cream color of standard E. coli. On the other hand, intact β-galactosidase...increases pigment precipitation, enhances the blue color of negative colonies, and allows for better differentiation...

...Science. Learn more by visiting the Allen Cell Explorer at allencell.org and the Allen Institute website...please visit the Cell Catalog on the Allen Cell Explorer, where you can also find the certificate of analysis...

...plasmid with Addgene, hoping to inspire others to explore the phenomenon, improve the tool, and develop new...powerful tool, it was promising enough to keep exploring and to try developing a variant with an enhanced...York Lab operates with a strong commitment to exploration and collaboration. Andrew is a physicist with...inventors and biomedical engineers, join efforts to explore the mechanisms and develop potential applications...

...of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration must be used ...and/or different IgG subclasses of 1° for multi-color staining Can be expensive Nanobodies   Recombinant...staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don’t require...application to the sample. Staining with this multi-color staining workflow yields similar localization patterns...