We narrowed to 95 results for: MLS

...Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes Before Starting Refer to ...Triton X-100 in 10 mL PBS. Blocking buffer: Dilute 0.5 g BSA and 30 µL Triton X-100 in 10 mL PBS. Antibody ...HeLa cells 24-well plate 4% Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum...buffer: Dilute 0.5 g BSA and 150 µL Triton X-100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 ...DAPI stock solution by diluting 2.1 µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. ...diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding...concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL of the diluted antibody to the wells ...

...cell lysate to a 4 mL tube. Add the following sequentially to 1 mL of lysate: Add 0.1 mL of 2 M sodium acetate...For tissues: use 1 mL of Solution D per 100 mg of cells. For cultured cells: use 1 mL of Solution D per... inversion. Add 1 mL water-saturated phenol, mix thoroughly by inversion. Add 0.2 mL of Chloroform/Isoamyl...For tissues: use 1 mL of TRIzol® per 100 mg of cells. For cultured cells: use 1 mL of TRIzol® per 1 X ...homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used. Shake vigorously...solution RNase free tubes: microcentrifuge tubes, 4 mL polypropylene tubes RNase decontamination solution... Wash the RNA by resuspending the pellet in 0.5–1 ml of 75% Ethanol and vortex for a few seconds. Centrifuge...

...Prepare 1X TBST as follows: 25 mL of 20X TBS 2.5 mL of Tween-20 472.5 mL of deionized water Mix well Prepare...running buffer as follows: Dilute 25 mL of 20X running buffer to 500 mL with deionized water. Mix well. Fill...water. To prepare 20% ethanol, dilute 2 mL of ethanol into 8 mL of deionized water and mix well. Assemble...Gently remove supernatant. Resuspend cell pellet in 1 mL of 1X PBS and transfer to a microcentrifuge tube....dilutions of the BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of standard, blank, ...into 1X TBST Example: 5 g of non-fat milk into 100 mL of 1X TBST. Pro-Tip The ideal blocking buffer will...between antibodies but is typically between 1–10 μg/mL. Incubate the membrane overnight in primary antibody...

...Potassium Acetate) 120 mL 5M Potassium acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution... of interest. Add 1.5 mL of the stock culture to a 1.75 mL microfuge tube. Centrifuge in microfuge tube...buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform...Solution III at 4°C Grow 2 mL overnight cultures from single colonies of bacteria containing your plasmid of... or carefully pouring. Optional: Add 5 μL of 2 mg/mL RNase A to the supernatant in the new tube and incubate...

...20 in PBS) as follows: Pipette 0.5 mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several...the wells. Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate...9018 Bovine serum albumin (BSA), VWR 10273-264 50 mL conical tubes, VWR 89039-656 96-well polyester (clear...150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518...1% BSA in PBS) as follows: Add 250 mg of BSA to 25 mL of PBS. Cap the tube and invert several times to ... BSA in PBS) as follows: Dilute 50 mg BSA into 50 mL PBS . Cap the tube and invert several times to mix...starting with three concentrations between 1–10 µg/mL. Cap and invert the tubes several times to mix. Using...

...target plate, add between 0.5–1 mL virus (add 0.5 mL for a high MOI, and 0.1 mL for a low MOI). Scale the amount...#7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use at 100 μg/mL. C.2 Annealing Oligos Resuspend...concentration of puromycin should be from 1-10 μg/mL in 1 μg/mL increments. d. Label plates from 1-10 and add...1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100 μg/mL antibiotic. Pour...protocol. Plate on LB agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog...Inoculate 5 colonies from each ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down ...to be transfected, plate 7×105 HEK-293T cells in 5 mL of media in a 6 cm tissue culture plate. Incubate...

...Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated protein; LST8 homolog LKB1 Also ... viral oncogene homolog FOXO Forkhead box O GBL/mLST8 MTOR associated protein; LST8 homolog GSK3 GSK3A...

...g of agarose in 100 mL of TAE will make a 2% gel). Mix agarose powder with 100 mL 1xTAE in a microwavable...approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr binds to the...Agarose Ethidum bromide (stock concentration of 10 mg/mL) Gel loading buffer, New England Biolabs B7022S Procedure...buffer, place the gel into a container filled with 100 mL of TAE running buffer and 5 μL of EtBr, place on ...

...10 12 GC/mL to >2 x 10 13 GC/mL and we use an internal reference virus that is 1 x 10 13 GC/mL. We recommend...1 x 10 12 GC/mL, use dilutions 3–6 If sample is expected to have a titer >3 x 10 13 GC/mL, use dilutions... add carrier DNA to a final concentration of 4 ug/mL to each standard dilution. Dilute DNase-treated and...the physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions...

...Sample Dilution Concentration Titer (GC/mL) Average Titer (GC/mL) AAV Sample 1 6400000 965 3.09E+13 3.2E...are based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents... $$T = {R*C*D*1000\over V}$$ Where: T = Titer, GC/mL R = Reaction volume (20 µL) C = Copies/µL D = Dilution...

...MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated protein, LST8 homolog MTOR Mechanistic...

...on your benchtop and typically hold the common 1.5 mL microfuge tubes used in labs, though some larger ...samples. For example, spinning down samples in 1.5 mL microfuge tubes at 10,000 rpm would require a tabletop...

...overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Notes:...

... in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place a microcentrifuge tube on the balance...

...dispense small amounts of liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing...