Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,010 results for: PLE;

Showing: 81 - 100 of 1010 results

  1. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...replicate.   How can I prevent recombination in my samples? Plasmid recombination can affect viral vectors...vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating elements...stable E. coli reduce the rate of recombination in samples. For a more detailed comparison of these cloning...following protocols for low copy plasmids. For example, using a longer growth period (up to 24 hr), reducing...rich media can improve growth and DNA yield. Test multiple, small colonies After streaking LB agar plates...cultures contain the recombined vector backbone. Test multiple colonies before saving a glycerol stock to ensure... easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that...

  2. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...ensure that samples are easy to locate in freezers that may be shared with multiple people or even multiple.... Some samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received...of the samples that come through our doors. It would be a nightmare if we didn’t have a simple way to ...every sample can be tracked easily at each step in the storage process. After the original sample is barcoded...scanned to verify that it is the correct sample. Tracking samples - it's all about location, location, location...these samples have the 2D barcodes mentioned above. These barcodes identify what type of sample is contained...allow us to store multiple types of tube for each plasmid we have at Addgene. Each sample at Addgene has ...

  3. p53 Pathway

    Type
    Collection
    ...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...

  4. Control AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  5. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ... one simple experiment. PCA is an in vivo technique where two proteins are fused to complementary reporter...neighboring proteins. Thus to capture protein complexes, scientists can simply express a bait protein ...suboptimal folding or stability. Thus they conducted multiple rounds of directed evolution until they identified...interactions Split-BioID combines a protein fragment complementation (PCA) technique with proximity labelling to...labeling and purifying additional proteins from a complex of interest. For more information, read the Split-BioID...detect RNA-protein interactions that can regulate multiple biological functions. RapID (RNA-protein interaction... and Paul A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions...

  6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ... have no fear - I’m here to introduce you to a simpler, yet more effective, CRISPR-based epigenetic approach...machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9 alone...Sapozhnikov and Szyf 2021).    Figure 3: A simpler approach - dCas9 blocks DNMT1 methylation, leaving...too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding is crucial for...blasticidin or puromycin resistance genes.  It’s that simple! Good luck and happy editing!    Thank you to...

  7. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...affected the pH of our viral samples. Read on to learn how aqueous samples might be affected by dry ice...Measuring the pH of our samples: What we did To measure the pH of our samples, we used a solution of cell...when the samples were frozen. This was clear by the gradual change in pH visible at the sample interface...this, we stored samples on dry ice for 72 hours and then vented the tubes by completely removing the caps...at -80°C. For samples that are shipped on dry ice, this can affect the pH of the sample, which may have...have some effect on the contents of the sample. For example, one study found that dry ice storage affected...describing how short term storage on dry ice can shift sample pH, we wondered whether or not the dry ice we use...

  8. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...Novophage to Sample 6. To learn more about Novophage's successful transformation into Sample 6 and Dr. ... in food products prior to Sample 6 16:27 - 18:02 : Benefits of the Sample 6 technology and how It works...- 19:56 : Why Sample 6's first test was for Lysteria 19:56 - 24:41 : Who is using Sample 6 and why? 24...Resources Visit the Sample 6 website Check out Dr. Koeris' Website, biotechstart.org Read Sample 6's Paper on...for use in a biotech startup 12:16 - 15:15 : Why Sample 6 began to develop tests for food products as opposed...Directors. We're excited to watch both Addgene and Sample 6 grow with his help! Special thanks to our sound... sample6...

  9. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    ...construction system for multiplex genome engineering. Image from Sakuma at al. (2015). Multiplex genome engineering... construction system for CRISPR/Cas9-mediated multiplex genome engineering is now available thanks to ...new tool in Scientific Reports in June. “The multiplexity is one of the most advantageous properties of...established way of making an all-in-one vector for multiplex genome engineering.” Now, they’ve filled that...Cas9-nickase vectors work successfully to target multiple sites in cultured human cells. While it has not...be applied to efficient viral vector-mediated multiplex genome engineering. The system now provides an...an efficient targeting strategy for multiplex editing of genomes. It will also allow simultaneous activation...

  10. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGATGGCGAGAACAGGGcggtggagtataacgcTGTGGGCCTGACGAAGGA Complement Factor H TAL3230 & TAL3231 TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA...TTTCCTCTCATAGTCAATattaattctctatttgGCTCAAAATGTCAGTAAA Plekho1b-1 TAL3142 & TAL3143 TCGTCAAAGCGGGGCCCTcaggatgccaatcaacAGCCTGTGCAGCCCGACA...

  11. Optogenetics AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ... to implement in practice because FRET depends on a number of hard-to-achieve factors. For example, FRET...Bimolecular Fluorescence Complementation (BiFC). What is bimolecular fluorescence complementation? Bimolecular fluorescence... TK (2006) Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the...often easier said than done. Fortunately, a complementary method exists for visualizing protein-protein... fluorescence complementation is a method for detecting protein-protein interactions that uses split fluorescent...fragments that individually lack fluorescence. When complementary FP fragments are fused to two hypothesized protein...interaction partners (PIPs), the FP fragments form the complete FP because the PIPs they’re tethered to bring ...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...or somatic) stem cells, can differentiate into multiple cell types, but are typically limited to related...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...

  15. Biosensor AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  17. Synthetic Biology - Metabolism

    Type
    Collection
    ... related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux...

  20. MAPK Plasmids

    Type
    Collection
    ...programs by acting as an integration point for multiple biochemical signals. In collaboration with numerous...
Showing: 81 - 100 of 1010 results