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We narrowed to 364 results for: Pol

Showing: 81 - 100 of 364 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ... how much they get paid. Institutional salary policies are one indicatator of how salaries may changes... 2014, 89% of institutions had a minimum salary policy: of those, 51% of institutions set their postdoctoral...National Postdoctoral Association Institutional Policy Report 2014). However, ~30% set their minima lower...lower. Furthermore, 7% did not enforce these policies. It is unclear how many postdocs are at each of ...only is this in direct contradiction to explicit policies set up other institutions (like University of ...

  2. Cre-lox system

    Type
    Collection
    ... metallothionein MT-I region including the polyadenylation site and several introns tet-hCMV Mammalian...Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA mCherry and Cre; expressed in excitatory neurons...

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...target protein, so they are referred to as polyclonal. Polyclonal antibodies have the advantage of more robustly...find more details about polyclonal antibodies on our Antibodies 101: Polyclonal Antibodies blog post. Monoclonal...advantages and disadvantages compared to each other. Polyclonal antibodies These antibodies are derived from ...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... a duplicated SV40 polyadenylation region instead of the synthetic polyadenylation signal present in pGL3...system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the light-inducible protein... accessible for many potential applications. Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3)...recruits up to 24 copies of a protein to a target polypeptide. Multimerization in the SunTag system occurs ... simplifies the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series...constitutive promoters, 2 includible promoters, 3 polyA terminators and various pieces for inducible expression...UAAs via a re-assigned stop codon (TAG). This polyspecificity enables the site-specific introduction of many...

  5. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... boxes Some vendors take back their expanded polystyrene (a.k.a. Styrofoam or cooler boxes). We get these...receiving temperature-controlled reagents. Expanded polystyrene will likely end up in a landfill, but at least...with CO2 emissions. NEB takes back expanded polystyrene boxes for free (in the U.S.). Their cooler boxes... 1976! Millipore Sigma takes back expanded polystyrene boxes for free (in the U.S.). After you receive...

  6. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... excessive PCR cycles or low fidelity Taq DNA polymerase, the construction of specific sequences for base...believed that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These...any subsequent steps. Using high fidelity DNA polymerases, I have never had any mutations introduced into...efficiency is highest when using high fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most...

  7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...just upstream of the final ITR.  polyA  The polyadenylation or polyA signal aids in the nuclear export...translation, and promotes RNA transcript longevity. The polyA is located at the end of the AAV genome, just upstream...

  8. Plasmid Cloning by PCR

    Type
    Blog Post
    ...use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes more important... million bp depending on the polymerase used. No matter which polymerase you use, it is important that...

  9. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... be shared across other proteins. Conversely, polyclonal antibodies - which are a heterogenous mixture...antibodies, their cost is higher than that of polyclonal antibodies. For example, it takes longer to develop...than it does to immunize an animal and recover polyclonal antibodies (Lipman et al., 2005). This is reflected...Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...help mediate their transduction, such as the polyarginine peptide described in Zhou et al and Kim et ...

  11. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ... Traditionally, they have been made as either polyclonal antibodies or monoclonal hybridomas, but those...those techniques have several drawbacks. Polyclonal antibodies produced in animals may be variable among...& Plückthun, 2015a).   Figure 1: (Top) Polyclonal antibodies are produced in animals and consist...Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional hybridomas.  First...

  12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...slow the polymerization of formaldehyde.  Note: Formaldehyde in solution will naturally polymerize and lose...paraformaldehyde (PFA) as the fixative. PFA is a polymer of formaldehyde that, on its own, is not a fixative...PFA is dissolved in solution and heated, it depolymerizes, producing formaldehyde. Often people will call...

  13. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...biomolecules to the gel and add gel components to polymerize. Step 3: digest the sample to allow for even ...components penetrate the sample, how well the gel polymerizes, and the level of digestion. All of these factors...of epitopes during sample anchoring and gel polymerization - issues that can be minimized through tweaks...tweaks to these steps. But even under ideal polymerization and digestion conditions, loss of signal often...

  14. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... both vector amplification with low fidelity polymerases and the cost of synthesizing longer oligos, which...simple cloning procedures. Use high fidelity DNA polymerase This will minimize random errors during PCR: ...aliquots of PCR master-mix, containing buffer, polymerase and dNTPs, can be stored frozen: just defrost...mutagenesis by placing homologous ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62...

  15. Plasmids 101: Protein tags

    Type
    Blog Post
    .... Arguably the simplest affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function...the tag size quickly – modifying the length a polyhistidine tract does not greatly alter the size of the...used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small...Solubility and Affinity Abundance of charged and polar residues improves solubility; good for antibody-...

  16. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...of the sample to that of a standard curve to extrapolate the concentration of antigen in the sample. A... present in the sample and is calculated by extrapolating from the standard curve. Because the primary... present in the sample and is calculated by extrapolating from the standard curve. Scientists often opt... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...

  18. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...developed to quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts... transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence...effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent aptamers Corn...PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore...

  19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially available...reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that...transcription. This is then amplified by PCR using Taq Polymerase and then transcribed to provide RNA substrates...instead of RPA. Unlike RPA, this technique uses Taq polymerase which is widely available and less expensive....

  20. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... biology and biotechnology purposes.  Once a polypeptide is biotinylated (more on this below) it can be...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated ...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...
Showing: 81 - 100 of 364 results