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We narrowed to 761 results for: Rest

Showing: 81 - 100 of 761 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...control and optimize expression of your gene of interest. Inducible: The recombinase may be fused to a ...inversion, deletion, or translocation of a gene of interest. Using Cre or Flp as examples, here are a few ...or "LSL" cassette) placed upstream of a gene of interest will prevent gene expression in the absence of... Switch: These constructs contain two genes of interest, Genes A and B. When Cre is absent, only Gene ...and type of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool... integration of a plasmid containing a gene of interest and another FRT site. Flp recombinase recognizes...resulting in the stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing...

  2. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Express gene of interest from the CMV promoter pCDH-EF1 72266 Express gene of interest from the EF-1 promoter... Express gene of interest from the CB promoter pCDH-PGK 72268 xpress gene of interest from the PGK (phosphoglycerate...Express gene of interest from the CB promoter pCDH-EF1s 72484 Express gene of interest from a truncated...respectively, and empty vectors to express your gene of interest under the control of a variety of promoters. Professor...kinase I) promoter pCDH-CMV4 72284 Express gene of interest from the CMV promoter. WPRE has been removed to...

  3. Bacterial Expression Systems

    Type
    Collection
    ...use bacteria as model systems to answer many interesting biological questions. Escherichia coli is the...Visualization To determine the location of your protein of interest within the cell, you will need to tag it with ...protein (FP) tags so you can express your protein of interest fused to FPs and monitor their localization in... spectra overlap. That way if your proteins of interest interact, they will bring both FPs together and... only exciting the donor. In BiFC, proteins of interest are fused to each part of a split FP. When the...split FPs that can be fused to your proteins of interest in bacterial FRET and BiFC studies. ID Plasmid...Two-Hybrid (B2H) System allows you to fuse proteins of interest to transcriptional regulatory elements that will...

  4. Protocol - Bacterial Transformation

    Type
    Protocol
    ...recommend that you plate 50 μL on one plate and the rest on a second plate. This gives the best chance of...also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation... how to transform E. coli with your plasmid of interest. Protocols...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...and are designed for a user to insert a gene of interest. They may contain tags or fusion proteins, a multiple...allow you to test the function of your gene of interest in a controlled environment under various conditions... C-terminal 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal Myc...an N-terminal GST tag fusion with your gene of interest pFastbac1 Nterminal GST - Baculovirus expression...cell lines in which the expression cassette of interest is incorporated into the host DNA. For mammalian...you can clone your regulatory element or gene of interest to create a luciferase reporter Promoter Measure...HA or myc-BioID2-MCS - To fuse your protein of interest to the N-terminus or C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3...

  6. Five Popular Model Organisms

    Type
    Blog Post
    ...between cancer cells, therapeutic drugs, and the rest of the body than studies in other model organisms...level and spatiotemporal expression of a gene of interest. This type of control over gene expression is ...continuously improved upon since then. If you are interested in these systems check out Gerald Rubin’s lab...specific plasmids (L4440) that contain your gene of interest (Fire et al., 1998). The discovery of RNAi in ...sensor, GCaMP6 (Nguyen et al., 2015). If you are interested in C. elegans plasmids and resources check out...

  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... easily gate on the unlabeled cells of interest while the rest can be excluded, or “dumped”. From left...fluorophore-conjugated antibodies directly bind to epitopes of interest that are found in the extracellular space. The...like bulk lympho-/splenocytes, you might only be interested in one of the various cell populations; for example...selection: labeling markers that your cells of interest do not have, and selecting for the cells that ...quick method of negatively selecting your cells of interest is magnetic bead-activated cell sorting, or MACS...An effective strategy to sort out your cells of interest via FACS using negative selection is called dump...Intracellular labeling Oftentimes, a marker of interest is not located on the cell membrane but inside...

  8. Genetic Code Expansion

    Type
    Collection
    ...acids (ncAAs). A plasmid containing the gene of interest with the modified codon (typically the amber codon...a non-canonical amino acid into the protein of interest: The non-canonical amino acid, which is generally...codons, this can be challenging. In E.coli , the rarest codon is the amber stop codon (UAG) and thus this...this codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon ...pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest 209738 pAB228i16 G1 PylRS Methanogenic archaeon...sites changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...you can clone your regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise...Retroviral expression of firefly luciferase and gene of interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral...Lentiviral vector for insertion of your gene of interest upstream of IRES-firefly luciferase. Bryan Welm ...expression of firefly luciferase and dTomato. A gene of interest can also be inserted into the plasmid. Malcolm...construct that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return ...

  10. Open Enzyme Collection

    Type
    Collection
    ...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...incorporation of phosphoserine into your gene of interest....choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030 origins or a low copy p15a...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated...identify candidate human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented...

  12. Deisseroth INTRSECT Collection

    Type
    Collection
    ... The following resources may be of interest for groups interesting in implementing intersectional experimental...strategies to define cellular sub-populations of interest, including dual-transgenic recombinase-expressing...testing of novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online...

  13. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...step 3.1.4 ), 5.0 μl restriction enzyme buffer (10x), 4.0 μl (20 U) BbsI restriction enzyme (5,000 U/ml)...for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after ... construct. Transfecting CRISPRs into Cells of Interest NOTE: This protocol involves the delivery of CRISPR...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...out my window and I saw the Dorr Mountain with the rest of the skyline of Acadia National Park. I was confused...molecular biologist with Biogen and is particularly interested in applying CRISPR for mouse genome editing. ...Zoology, Chinese Academy of Sciences, and he is interested in using CRISPR to develop therapeutic applications...

  16. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander...encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These...

  17. Plan Your Experiment

    Type
    Collection
    ...manipulation to model a specific disease or process of interest. Do you want to: Generate complete and permanent...Intereference : Target the promoter region of the gene of interest, where the Cas9 protein can block transcription... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ...Some prime and base edits Can be paired with restriction enzymes for further customization Usually paired...

  18. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...it's important to determine where your protein of interest resides. There are a variety of methods for determining...to compare the localization of your protein of interest with known markers. Addgene has assembled a collection...subcellular structures. You should fuse your protein of interest with a fluorescent protein that has a different...localization or possible function of a protein of interest. Browse or search the table below to find markers...

  19. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast.... overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization of some of...tagging vectors to create fusions of proteins of interest with yeast codon-optimized fluorescent proteins...

  20. p53 Pathway

    Type
    Collection
    ...best-characterized effects of p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The... receptor Gadd45 GADD45A GADD45B GADD45G Growth arrest and DNA-damage-inducible; alpha, beta, or gamma...BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5...
Showing: 81 - 100 of 761 results