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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: STI

Showing: 81 - 100 of 1045 results

  1. Rett Syndrome

    Type
    Collection
    ...syndrome is currently based on specific clinical diagnostic criteria, defined as the presence of relatively...gait. Greater than 95% of individuals who meet diagnostic criteria have disease-causing mutations in the...switched on regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could...that can be differentiated into neurons Coriell Institute: Patient-specific lines (Link opens in a new window... database of clinical features and genetic characteristics of Rett syndrome (Link opens in a new window...Repositories (Link opens in a new window) Coriell Institute - cell line repository, including patient-derived...18337588 Neul et al. 2010. Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol . 68, 944...

  3. TALEN Guide

    Type
    Collection
    ...but they still have potential targetable sites approximately every 35bp. Researchers are still determining...dimer, cutting in the spacer region between two distinct ZF target sites. (B) A TAL effector nuclease (...available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted...Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available from Addgene before the end...to mutate a specific gene or your anxiously investigating gene therapy options for a rare disease in humans...constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia...

  4. All Antibodies

    Type
    Collection
    ...feedback and suggestions for new antibodies to add to the collection using our Antibody Suggestion Form . Browse...recommended applications based on our in-house testing and data provided by trusted outside labs – with...targeting proteins for neuroscience research. Institute for Protein Innovation Collection : Antibodies...Recommended Applications PI Return to Top Do you have suggestions for other antibodies that should be added to ... represent the official views of the National Institutes of Health. Need Some Help? Browse some of our...

  5. AAV ddPCR Titration

    Type
    Protocol
    ...ensure that you are in compliance with your institution’s biosafety regulations. Equipment Class II, Type... When analyzing data there should be a clear distinction between negative droplets (black) and positive...

  6. Kit Free RNA Extraction

    Type
    Protocol
    ...entire tube is dried but while the white pellet is still visible. Resuspend RNA pellet in RNase-free water...

  7. E11 Bio PRISM Collection

    Type
    Collection
    ...with the delivery of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits...plasmids expressing the barcode proteins under the constitutive CAG promoter (Addgene #242764–242781), but E11...individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled AAVs delivered in vivo generates...window) , in collaboration with The Francis Crick Institute (Link opens in a new window) , MIT (Link opens...opens in a new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz...hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription Pete Stecha...212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription Pete Stecha...Expression Constructs Luciferase driven by a strong constitutive promoter can be used to monitor transfection...NF-kb, TGF-b, c-Myc, p53, and MAPK/JNK plus a constitutive control. Koen Venken 133315 pMKV060 Firefly ...proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin... live imaging of mTOR signaling. Do you have suggestions for other plasmids that should be added to this...

  9. AAV Packaged on Request

    Type
    Collection
    ...such as titering, purity assessment, sterility testing, and viral genome sequencing.... process: Titering Purity assessment Sterility testing Viral genome sequencing (VGS) Find detailed pricing...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that...order. MTA Approval 1+ business days, varies by requesting organization Viral vector preps remain subject... typically takes 1–4 days, depending on your institution. AAV Production 6–8 weeks Quality is our priority...Addgene does not perform functional or in vivo testing. We recommend that you consult the original paper...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein...the Zhang Lab established Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ....sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV...from Jackson Labs: Cre-dependent Cas9 mouse Constitutively active Cas9 mouse Available plasmids are described...plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed and...

  11. Open Enzyme Collection

    Type
    Collection
    ...site) 165508 pOpen-HindIIIR HindIII 165526 pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create...pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI...basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top...

  12. Genetic Code Expansion

    Type
    Collection
    ...GCE bulletin board (GCEbb) listserve and posting your questions there. This peer-to-peer GCE community ...non-canonical amino acid can be incorporated using the existing protein translation machinery. To expand the genetic...a protein so that it is responsive to specific stimuli, such as light. On a broader scale, the expanded...made when the ncAA is included. For any and all questions you have while designing, implementing, or troubleshooting...127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted phenylalanine derivatives and tyrosinyl ethers...pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine analogs Mammalian TAG Huiwang Ai...reassigned for multi-site incorporation of two distinct non-standard amino acids. Derived from C321.deltaA...

  13. Antibody Production

    Type
    Collection
    ... distributed by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm..., freezing at -80 °C, thawing on ice, and then testing for loss of potency against a non-treated control...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However, Addgene...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...defines 5 types and 16 subtypes based on shared characteristics and evolutionary similarity. These are grouped...easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple...researchers to further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in ...:1479-91. PMID: 24360272 Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg.... Science . 337(6096):816-21. PMID: 22745249 Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT...effects. Nature . 529(7587):490-5. PMID: 26735016 Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng...

  15. Distribution to Industry

    Type
    Collection
    ...HR700_TP53 Exon Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...execute legal documents on behalf of the recipient institution. Once the MTA has been signed, it can be submitted...

  16. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64...vector, CMV promoter, Hygro Campeau Viral Service Suggestions Don’t see what you are looking for? Please let...viral format by filling out our Viral Service Suggestion Form (Link opens in a new window) . Please note...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal...Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids with four promoters, six fluorophores...resource center based in China focused on collecting existing zebrafish resources and developing new lines and...Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish...genomics in zebrafish. Return to top Do you have suggestions for other plasmids that should be added to this...

  18. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Mazzone CM, D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham N, Cristiano C, Dorrier...following resources may be of interest for groups interesting in implementing intersectional experimental design... in a new window) for the molecular design and testing of novel INTSRECT plasmids for groups interested...Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the...

  19. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome...phenotype. The National Human Genome Research Institute (NHGRI) is funding this collaborative program ...program that brings together teams of investigators who will use state-of-the-art experimental and computational... funded by the National Human Genome Research Institute (NHGRI) (Link opens in a new window) . Find IGVF...

  20. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...teaching different types of courses, at any type of institution, and at different points in their careers to ...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...
Showing: 81 - 100 of 1045 results