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We narrowed to 728 results for: STO;

Showing: 81 - 100 of 728 results

  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ... collection. Addgene also has a selection of in-stock viral preps available. Browse Guides and Plasmids...

  3. Distribution to Industry

    Type
    Collection
    ...SUMO Protease Elution Protein Characterization Christopher Bahl A validation set of 96 unique, single-domain...

  4. Promega Plasmid Collection

    Type
    Collection
    ...visualization, and functional studies. You can even customize ligands for your specific purpose. HaloTag technology...

  5. Plant Plasmids and Resources

    Type
    Collection
    ...reproduces, and distributes diverse seed and other stocks of Arabidopsis thaliana and related species. The...

  6. Lentiviral Prep Service

    Type
    Collection
    ...Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding...

  8. COVID-19 Resources

    Type
    Collection
    ...Cytokines - SARS-CoV-2 infection induces a cytokine storm response in the body. For a full list of cytokines...

  9. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated...

  11. Retrograde AAV viral preps

    Type
    Collection
    ...- pAAV SYN1 HA-hM3D(Gq) Syn Activator DREADD Christopher Richie 50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn Inhibitor...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Interest Click on different properties to create a custom filtered list of the CRISPR pooled libraries in...

  14. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...lung, the heart, and the abdomen. Long story short, when asbestos is breathed in (or less frequently, ingested...small, hook-like shape of asbestos fibers. Over time these embedded asbestos fibers cause inflammation...known safe level of exposure to asbestos – i.e., any exposure to asbestos is potentially capable of causing...exposed to asbestos but have not developed the cancer yet Those who have not been exposed to asbestos Tests...greatly decreased through a ban on asbestos. Nonprofits like the Asbestos Disease Awareness Organization ...bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that future generations...completely preventable. Mainly caused by exposure to asbestos, mesothelioma is a form of cancer that develops...

  15. Tips for Technical Support Calls

    Type
    Blog Post
    ...Having spent that long in customer service, I've daydreamed about the ideal customer service interaction -...to any biology-related customer support interaction. Before contacting customer service In an age of Tweeting...answered hundreds of phone calls and thousands of customer service emails in my six years as a senior scientist... - one that gives our customers the most utility in the least amount of time. Though I now spend far less...my years of accumulated wisdom so that you, the customer, can get the most out of your email or phone call... bad product (in which case, you should probably stop using that company). Frequently asked question pages...section are really, truly frequently asked by customers (and I expect this is the case at most companies...

  16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results showed that...mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...reading a publication describing how short term storage on dry ice can shift sample pH, we wondered whether...freezer and then moved them to a closed dry ice storage container for 48 hours (to replicate how our viruses...from the dry ice). After 48 hours of CO2/dry ice storage, we then thawed the samples at room temperature...thawed, there was no effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once...

  17. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...then inoculated for storage on Thursday. The lab prepares bacterial glycerol stocks on Fridays. From each...used for long term storage (one is kept at Addgene and another is sent to a storage facility). The third...courier and then the journey of transformation and storage begins. Some samples are submitted as bacterial...intact. Some deposits are sent from labs nearby in Boston and Cambridge. Many deposits arrive from labs around...sample can be tracked easily at each step in the storage process. After the original sample is barcoded,...entry into Addgene’s repository. For long-term storage in our facilities, every sample is transferred ... - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control processes...

  18. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...support through the customer’s perspective is good. If you’ve never participated in a customer support conversation...the types of questions customers ask, and there are also differences in customers’ personalities and their...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems... product issue in the lab. The issue was with a custom-made reagent to manipulate cells (manufactured ...multiple languages - that makes communication with customers from around the world easier. You should also ...expectations. If you are willing to listen to a customer’s concerns and are patient enough to find the real...are the most popular ways to communicate with customers. Online live chat is a convenient alternative ...

  19. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...amber (UAG) stop codon by glutamine insertion supF (tyrT)   Suppression of the amber (UAG) stop codon by...or protein expression. If you were to include customized strains, the number is probably in the thousands... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred...Streptomycin MC1061 derivative. General cloning and storage, blue/white screening, leucine auxotroph. F- endA1...mrr-hsdRMS-mcrBC) λ- DH5alpha   General cloning and storage of common plasmids, blue/white screening. F- endA1...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    ...grow this initial stock and create a set of vials of ‘Master’ stock, which can be stored in liquid nitrogen...some of these vials will be stored in a separate backup liquid nitrogen store to prevent loss from equipment...used to produce a set of ‘working’ stocks of cells. Working stocks should be frozen in multiple vials ...plenty of maintained freezer stocks. It is vital that the Master and Working stocks of cell lines have the ... monitor the stock levels you have in liquid nitrogen for your Working and Master stocks of each cell ...and money but cells lose provenance (this is the history of the cell line consisting of records of the origin...used up. This ensures you can retain the maximum stocks and use of the original Cell line you purchased...
Showing: 81 - 100 of 728 results