... scientific review of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid ...

... novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital...

...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated...

...Cytokines - SARS-CoV-2 infection induces a cytokine storm response in the body. For a full list of cytokines...

... Listed - a CRISPR Screen Tool Design gRNAs for custom CRISPR screens targeting a long or short list of...

...general transfection, you could use 1ul of 10uM oligo stock for each well on 24-well plate, co-deliver into ...

...application, and in such cases, it may be necessary to customize an existing reagent to suit your needs. Genetic...

... N. 2019. Excitation of diverse classes of cholecystokinin interneurons in the basal amygdala facilitates...

...Interest Click on different properties to create a custom filtered list of the CRISPR pooled libraries in...

...Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI...

...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...complete. HRP-conjugated antibodies should not be stored in sodium azide, as it acts as an inhibitor to ...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...

...2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute your...media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) to 100 mL of LB. DNA sequencing for plasmid...bacterial cells that have been preserved as glycerol stocks. Functional in many species and can be useful for...synthesized RNA signals for the transcription process to stop. An expression vector can also include an enhancer...media or LB agar plates. Antibiotic Recommended Stock Concentration Recommended Working Concentration ...place of ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated... acid. There are 20 amino acids plus a start and stop codon. Below you will find helpful resource tables...

...phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based on ... new window) Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015). The First Structure–Activity...46. PMID: 25937170 (Link opens in a new window) Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ... Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 28:112-126.e6....

...general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells...cells that package DNA into nucleosomes. Histone binding prevents the initiation of transcription whereas...

...their recognition site allows for the creation of custom overhangs, which is not possible with traditional...have utilized this approach to create compatible custom overhangs that can then be efficiently assembled...fragments of DNA that you would like fused along with custom ordered DNA oligos of 60-80 base pairs in length...

...insertions, or frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the... cytosines in a gene’s promoter or by inducing histone acetylation or demethylation. The most common modifiers... green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy...gene by shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA...

...supernatant containing the virus is removed and stored or centrifuged to concentrate virus. Crude or concentrated...transgene Purpose Envelope VSVG in trans Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ...

...lung, the heart, and the abdomen. Long story short, when asbestos is breathed in (or less frequently, ingested...small, hook-like shape of asbestos fibers. Over time these embedded asbestos fibers cause inflammation...known safe level of exposure to asbestos – i.e., any exposure to asbestos is potentially capable of causing...exposed to asbestos but have not developed the cancer yet Those who have not been exposed to asbestos Tests...greatly decreased through a ban on asbestos. Nonprofits like the Asbestos Disease Awareness Organization ...bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that future generations...completely preventable. Mainly caused by exposure to asbestos, mesothelioma is a form of cancer that develops...

...Having spent that long in customer service, I've daydreamed about the ideal customer service interaction -...to any biology-related customer support interaction. Before contacting customer service In an age of Tweeting...answered hundreds of phone calls and thousands of customer service emails in my six years as a senior scientist... - one that gives our customers the most utility in the least amount of time. Though I now spend far less...my years of accumulated wisdom so that you, the customer, can get the most out of your email or phone call... bad product (in which case, you should probably stop using that company). Frequently asked question pages...section are really, truly frequently asked by customers (and I expect this is the case at most companies...

...pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results showed that...mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...reading a publication describing how short term storage on dry ice can shift sample pH, we wondered whether...freezer and then moved them to a closed dry ice storage container for 48 hours (to replicate how our viruses...from the dry ice). After 48 hours of CO2/dry ice storage, we then thawed the samples at room temperature...thawed, there was no effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once...