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We narrowed to 939 results for: TIM

Showing: 81 - 100 of 939 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

  2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the... problem with this is that it may take months of time for your manuscript to get reviewed and edited prior... a protocol as you finish them, turn on pre-set timers for incubation steps, and save the entire process...

  3. CRISPR Plasmids - Plants

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  5. Lentivirus Plasmids

    Type
    Collection
    ... EGFP. Can be used for cDNA expression. David Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV ...Gearhart 44333 pMX_mTBP TBP LTR Parkinson's Marc Timmers 44978 GST-FUS-WT FUS GST tac ALS Robin Reed 44981... CMV DJ-1 C106A PARK7 mTagBFP2 CMV Parkinson's Timothy Ryan 221331 pFastBac-GST-TBK1 TBK1 His, TEV, GST....1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin-1-145 SYNJ1 His...

  7. Brain Armamentarium

    Type
    Collection
    ..._3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) Optimized expression of SYFP2 (yellow fluorescent protein...

  8. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research story, I ...couldn't resist adding in this excellent Q&A from Tim Miles on everything from AAV prep to targeting to...

  9. Summer Fun at Addgene!

    Type
    Blog Post
    ...AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head on over to our ...our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted questions...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...students. Many thanks to our guest blogger Tim Herman! TIm Herman is the director of the MSOE center for...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...become real”.  We sometimes forget that the models we create are around 30 million times larger than the...Genome Visualization Tips for a First Time CRISPR User by a First Time CRISPR User Resources on Addgene....

  11. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...former colleagues! I ran into past ASM President Tim Donohue (see below) on my way back from getting a...Research Center and a fellow Rhodobacter enthusiast, Tim also hosted me at his University of Wisconsin lab... annual meeting (ASM Microbe 2016) for the first time. Our goals at the meeting were to network with scientists...this Broad Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several...

  12. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from...grateful for your writing, your knowledge, and your time. ...

  14. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...without the artifacts sometimes associated with plasmid-based overexpression. Tim Formosa, at the University...—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (...series of blue, green, and red FPs that are codon optimized specifically for expression in yeast. These tagging...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized... output requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or...

  15. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep pGP-AAV-syn-flex-iGABASnFR2...

  17. Hot Plasmids - February 2022

    Type
    Blog Post
    ...set of anti-HA frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi Kimura’s labs have recently...Frankenbodies for modular imaging of cellular targets Optimized genetic screening technique using Cas12a for multiplexed...https://doi.org/10.1038/s41467-019-10846-1.   Optimized genetic screening technique using Cas12a for multiplexed...have expanded the genetic screening toolkit by optimizing a Cas12a-based method for screens. Cas12a’s ability...increase the potential of Cas12a genetic screens by optimizing enzyme activity using an enhanced Cas12a variant...from DeWeirdt, et al. 2021 To validate this optimized genetic screening technique, researchers identified...simplicity for combinatorial genetic screens, and this optimized toolkit furthers Cas12a’s potential in future ...

  18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ... to a startup Mike Koeris and fellow co-founders Tim Lu and others first came up with the idea that would...raised $6 million. The biggest challenge during this time was learning to communicate effectively to non-scientific...complications and potential conflicts that waste money and time. Most importantly, “the company does not become ...become real until someone takes the risk full-time and abandons all safety nets.” Raising seed money. “Before...formerly VP Operations of Sample6) for taking the time to speak with us about the biotech startup process...

  19. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...Currently, Aggarwal, and colleagues Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 ...Campus, HHMI where he works on developing and optimizing biosensors in the lab. He has recently developed...sensory input from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory... demonstrate pain response due to blue light stimulation of dual virus-expressing CR neurons. (C) Duration...biosensors.   This screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human cells using the...References 1. Cheng, Allen A., Huiming Ding, and Timothy K. Lu. "Enhanced killing of antibiotic-resistant...PMCID: PMC4151723. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize...
Showing: 81 - 100 of 939 results