...CRISPR Blog Topic Page: CRISPR tips, FAQs, and news featured on Addgene's blog Protocols Lab(s) Description...

... must be delivered intracellularly in order to measure luminescence. Gaussia luciferase is secreted and...

...Genetic Tools Atlas (Link opens in a new window) . Figure 1: Selection, prep, and testing of AAV enhancers...

...wide variety of Cre recombinase plasmids. Special features include: Inducible Cre: These constructs require...

...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS Trina ...177653 pT7II-2N4Rtau MAPT T7 Parkinson's, FTD Jeff Kuret 177840 pCDNA3-IgL-C99-T4L-rTA APP CMV Alzheimer'...

...experiment. Figure 2: Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE CAPTURE 2.0 Biotinylation...between original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased.... Find plasmids for CAPTURE 2.0! When compared to the original CAPTURE, CAPTURE 2.0 had a ~14-fold increase...Recently the Xu lab developed CAPTURE 2.0, an updated version of CAPTURE that detects chromatin interactions...Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation of a.... From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin interactions...next-generation sequencing. Figure 1: Overview of the key steps of the CAPTURE method. After protein and...

...Download ReFigure extension and visit ReFigure.org. You can reach the ReFigure team at refigure@refigure.org...You can use ReFigure to coherently save analyses with the relevant figures from a literature review. No...currently supported by ReFigure beta) you can include it in a ReFigure. ReFigures are good for you and ...support ReFigure by creating ReFigures and answering this 2 minute survey! You can also find ReFigure on Twitter...reading scientific literature? Very often the data of interest are in 1 or 2 figures from one paper and...performance as in this antibody ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium...The ReFigure extension also tells readers if a paper they’re reading has been linked in a ReFigure making...

...Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics...Outreach Scientist Nick Amiss. Cell culture in the present day Cell culture is a widespread tool used in the...surrounding cell culture over the last few decades some pharmacologists have tended to avoid cell culture in favour...artefacts. The life science literature is a litany of false interpretation in cell culture for this reason. Unreproducible... conduct high quality cell culture. Biorepositories around the world ensure that the cell lines deposited...Type Culture Collection aka ATCC where I work. It is virtually impossible to work in cell culture, microbiology...benefited most as cancer cells are amenable to culture in vitro. Virologists too have benefited from the...

... causes this culture shock and how to overcome it. What causes the lab → office culture shock? Working...her (3 weeks in and there's a big office vs. lab culture shock). I'm looking to see if there are any resources...help with the transition.” The lab-to-desk-job culture shock is a real thing. I experienced it, and many...in an academic research lab involves a few key features that can be difficult to replicate in other work...provided a few counterpoints to some of the causes of culture shock above, but after polling some Addgene colleagues... possible. It’s in their best interest to do so, sure, but it also eliminates that feeling of floundering... need to be in the lab that long because of the nature of your time course, but you’ll inevitably have...

...15 minutes before you start any tissue culture work and make sure that clean air is flowing in. The vent...contaminated. Make sure cells don't stay out too long While you’re preparing things, make sure your cells don...Colloids and Interfaces. Here are some tips for cell culture that will hopefully help you keep a well organized...in-flowing air is normally visible and you should always ensure nothing is covering it. Turning on the UV light...microorganisms that it strikes directly. UV light exposure is also harmful for eyes and skin so keep the ...while work is in progress.Learn more about cell culture contamination in this post from the ATCC Declutter...the laminar air flow around your work area. Make sure to only bring required materials into the hood. ...

...specific guidance on grammatical errors, sentence structures, and overall format (fonts, font size, legibility...the perfect opportunity to paint a comprehensive picture of your academic interests, potential, and drive... True! But you create a network of friends, and future colleagues that can help pull one another up while...

...double-stranded DNA cleavage. Nature 533:420–424 . https://doi.org/10.1038/nature17946 Lenay C (2000) Hugo De... on the depiction of genome engineering in pop culture SciFi is one of the most imaginative and most important...fiction. It has always served as a vision of the future and to make us reframe our perspective of the current...in the field of health sciences, energy, and agriculture, its portrayal in popular fiction has been largely...editing without double-strand breaks or donor DNA. Nature 576:149–157 . https://doi.org/10.1038/s41586-019...MacLeod CM, McCarty M (1944) STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL...A•T to G•C in genomic DNA without DNA cleavage. Nature 559:E8–E8 . https://doi.org/10.1038/s41586-018-...

...registration please go to http://futureofresearch.org/boston/. The Future of Research Symposium A year ago...daily lives and future prospects. Among the topics discussed, many related to the structure of the biomedical... overall structure of the biomedical research sector, and what a tenable workforce structure may look ...biomedical graduates take in their future careers. To participate in the Future or Research Symposium Boston...The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has ...has been contributed by guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here... of the six panel discussions to be held at the Future of Research Symposium Boston 2015 – Academic Data...

...upcoming "Future of Research" conference. You can register for the conference here. The Future of Research...colleagues in envisioning the Future of Science. Attend the Symposium on the Future of Science: A postdoc-...science to be happy. What do we have to change to ensure that all scientists can have successful careers...postdoc-organized symposium on the future of the scientific endeavor October 2-3, 2014 Boston University Jacob...

... Elite: Nobel Laureates in the United States. Free Press. New York. Resources MDH CUREs Community (MCC...number of honorees to have trained under a previous laureate (Zuckerman, 1977; Ma et al., 2020). Many have ...trainees at an earlier point in their careers? A CURE for Biochemistry Undergraduate biochemistry courses... Course-based Undergraduate Research Experience (CURE). Having incorporated a discovery-based lab on planarian... a stable, easily purified protein with easily measured activity, MDH is particularly amenable to in-class...    Fig. 1: Undergraduates in Provost’s CURE prepare to analyze their recently purified MDH to...the impact of phosphorylation on the enzyme’s structure, function, and interaction with other proteins...

...protein variants. The future of DNA feature identification What’s next for common features? We plan to continue...creating a database of common features, and devising rules for identifying a feature even when the match is ...is imperfect. SnapGene's feature database The source of common features was our collection of popular ...identity when detecting a reference feature. For a coding sequence feature that may be used to make fusion... into the original literature, some of it decades old, to provide reliable feature annotations. Even with...that a feature is missing from our database, or if you see an opportunity to harness common feature detection... planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning ...

...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...takeaways I got from the symposium. The goal of the Future of Research Symposium On the FOR website, the FOR...prevent us from identifying problems with the structure of the academic research workforce, and we are...only 14.3% of PhDs in the life sciences end up in tenure track position 5 years after receiving their PhDs... needs to be asked going forward is: How do we measure inclusion and equity? This is a difficult question...capturing, quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined...Stephan’s Book - How Economics Shapes Science The Future of Research Website Additional Publications of ...

...office space on the nicely manicured lawn of University Park at MIT.  I’m sure at some companies, employees...those early years, the founders knew that company culture and enjoying your job were important parts of team... team buidling - they relieved some of the pressure of start up life and maintained their sanity by throwing... still isn't time for goofing off, the company culture here at Addgene has remained paramount. Candidates...Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best place to work, we do SO many...over 60 people join the fun - including a lot of future Addgenies as shown in the photo below.  Kids and... was another successful event! Addgene company culture At Addgene we organically developed the philosophy...

...relief that I attended the amazing, postdoc-driven Future of Research Conference at Boston University last...postdocs are taking some steps to control their own future in science (see my previous blog on this).  Indeed...writing about issues in our science training infrastructure for some years.  He spoke eloquently and vehemently... the need to pay postdocs more.  What will the future of research be? The second day of the conference...conference organizing team.  The topics were Training, Structure of the Workforce, Metrics and Incentives and Funding...beginnings of solutions can’t come too soon.  Watch the Future of Research Website for news and updates on the...

...power of light. In a recent article published in Nature Methods, Boyden and his team describe the discovery... or pathology, and by disabling neurons you can figure out what they are necessary for. We published our...branch on the tree of life. Our most recent paper in Nature Methods offers the first true two-color pair. We'll... to see how a monkey changes decision making, to cure a human patient. We can't do it all by ourselves...Distinct Neural Populations. Nathan Klapoetke et al. Nature Methods. 2014 Feb 9. (PubMed). Read More: Other...