...Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective brightness...

...RESource (EXPRESs) Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like factors...

...light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified...

...email: [email protected] We have found that a variety of Cas9-mediated genome modifications can be efficiently...

...The plasmids available in this format include a variety of popular plasmids and backbones with high cloning...

...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based on DNA...for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It is also... plating on the appropriate antibiotic and try varying the amount of recipient plasmid to insert in the...

...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...

...fluctuations and to minimize well-to-well variability that result from a variety of causes, such as pipetting error...multichannel pipet to minimize pipetting error and variability Mix samples very well by pipetting back and forth...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter...

...P8833 Target cells Varies based on your experiment Culture media for target cells Varies based on your experiment...to 20 μl serum-free OPTI-MEM TIP: You may want to vary the ratio of shRNA plasmid, packaging plasmid, and...experiment Materials for assay Varies based on your experiment Detailed protocols for preparing polybrene...

...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based on DNA...for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It is also...your transformation worked. You could also try varying the amount of recipient plasmid to insert. Isolate...

...optimal concentration of primary antibody to use will vary and needs to be empirically determined. If possible...buffer. Pro-Tip The ideal antibody concentration will vary and needs to be empirically determined. We suggest...concentrations. The ideal range of the standard curve will vary between targets and needs to be empirically determined...

...results in a heterogenous polyclonal population that varies in the number of integration events and the site...culture has, in theory, been transduced. There are various schools of thought on using selective medium throughout...monoclonal lines generated using this protocol will vary based on several factors, including the growth properties...

...several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to be representative but not exhaustive...DNMT1 Hereditary sensory neuropathy type IE Paul Khavari 25044 pDEST53-LRRK2-WT LRRK2 GFP CMV Parkinson'...pCB268 APP Flag, HA, Myc CMV Alzheimer's Alexander Varshavsky 53810 SYNJ1-145_pCSdest SYNJ1 CMV, SP6 Parkinsonism...Hereditary diffuse leukoencephalopathy Markku Varjosalo 187827 pGST2-GST-TEV-OPTN S177D S473D OPTN GST-TEV...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter...

...are engineering zinc finger arrays targeted to various zebrafish genes. The table below lists zebrafish...

... in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation...

... E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms. See...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter...