.... Next, consider the expression level of your construct: would you like to stay as close as possible to...Finally, you can add bells and whistles to your construct. If you are planning to track your protein in ...

...experiments without having to clone a whole new construct for every application. Plus, the photophysical...fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures or low...easy! Instead of taking the time to clone a new construct for every new-and-improved fluorescent protein... so many possibilities from just one or a few constructs, it’s no wonder that self-labeling protein tags...

...Zhang lab created an all-in-one SaCas9 and sgRNA construct using the liver-specific serotype AAV8. Since ...control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome modification, but they did so ... the catalytic domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation ADAR2DD(delta984...

...multiplicity of infection (MOI) during cell library construction to ensure that the majority of cells receive...truncating one of the recombination sites in the construct to different lengths, the density of cells that...

...traditionally used Sanger sequencing to validate these constructs, as the relatively low cost and quick turn-around...suited to large-scale de novo assemblies of short constructs like plasmids. The plexWell™ workflow also offers...fragments within a library allows researchers to reconstruct the full individual sequence. Traditional NGS...

...the design and construction steps are complete, it’s time to make virus! The constructed viral vector containing...

...fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision, the resulting...the FP-SEC vector using Gibson assembly. This construct will serve as the repair template plasmid to create...

...confirm that you are working with the correct construct, and verify that the plasmid you received matches...and accurately visualize the full size of your construct. Consider buffer and temperature compatibility...

...switch CMV Mammalian Livet loxP (floxed) Constructs These constructs allow for Cre-regulated gene expression...Depending on the construct, Cre may activate or repress gene expression. Common construct types include:... can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre can excise the shRNA ...lox-STOP-lox shRNA constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs contain two...Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References...recombination principles, scientists have developed constructs to activate/inactivate genes when Cre is present...expression of your gene of interest. Many Cre constructs also contain fluorescent labels that indicate...

...A>G or T>C mutations in the plant genome. We constructed three versions of the vector (pKABE6.3, pKABE7.9...Agrobacterium-mediated transformation. We also constructed smaller vectors suitable for transient assays...require other base editor platforms. Hua et al. constructed a number of plant base editors replacing the ...

... to infect a cell is dependent on its capsid construction and the cell it’s infecting, getting these particles...not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer...

...permit five-color fluorescent imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc...photobleaching and phototoxicity are a concern, these constructs come in very handy. This approach is especially...

... common E. coli histidine kinase. This hybrid construct senses light, and an obligate chromophore allows...intensity. In addition to these improvements, the constructs have been greatly streamlined; only two plasmids...

... Figure 1: (a) Anti-FLAG frankenbody (FB) construction from the complementarity determining region ...and HA tags, you may not even need to clone new constructs to label your favorite protein! While the target...

...experimental work, spend some time outlining the construction of the plasmid and all the steps you will have...used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds of publications...

...suppresses neuronal transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to ...immune genes into pXPR_053. They expressed this construct in bone marrow stem cells from Cas9-expressing...

...cells.  Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve ...cells are then transfected with several different constructs for the reporter to work and to make the desired...

...experiments. Manufacturers rarely provide the construction plans or CAD (computer aided design) drawings... that allow for on-going creativity with the construction plans. Appropriate open-source licenses for ...

... plasmids episode! New, highly efficient RNAi construct for C. elegans gene knockdown Article contributed...phage display selections. In addition to these constructs, the Sidhu lab has made hundreds of additional...

...Cre-lox technology, transgenic mice have been constructed that express Cre under a variety of ubiquitous... many loxP-containing alleles have also been constructed. Additionally, Cre-containing adenovirus (Ad-...