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We narrowed to 310 results for: construct

Showing: 81 - 100 of 310 results

  1. Hot Plasmids - October 2022

    Type
    Blog Post
    ... states in deep tissue, in vivo. Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven... (AAV1) - and you can also find several other constructs in plasmid form. Fig. 1: Summary of the...

  2. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    .... Next, consider the expression level of your construct: would you like to stay as close as possible to...Finally, you can add bells and whistles to your construct. If you are planning to track your protein in ...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...experiments without having to clone a whole new construct for every application. Plus, the photophysical...fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures or low...easy! Instead of taking the time to clone a new construct for every new-and-improved fluorescent protein... so many possibilities from just one or a few constructs, it’s no wonder that self-labeling protein tags...

  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Zhang lab created an all-in-one SaCas9 and sgRNA construct using the liver-specific serotype AAV8. Since ...control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome modification, but they did so ... the catalytic domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation ADAR2DD(delta984...

  5. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...traditionally used Sanger sequencing to validate these constructs, as the relatively low cost and quick turn-around...suited to large-scale de novo assemblies of short constructs like plasmids. The plexWell™ workflow also offers...fragments within a library allows researchers to reconstruct the full individual sequence. Traditional NGS...

  6. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...the design and construction steps are complete, it’s time to make virus! The constructed viral vector containing...

  10. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...A>G or T>C mutations in the plant genome. We constructed three versions of the vector (pKABE6.3, pKABE7.9...Agrobacterium-mediated transformation. We also constructed smaller vectors suitable for transient assays...require other base editor platforms. Hua et al. constructed a number of plant base editors replacing the ...

  11. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...permit five-color fluorescent imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc...photobleaching and phototoxicity are a concern, these constructs come in very handy. This approach is especially...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... to infect a cell is dependent on its capsid construction and the cell it’s infecting, getting these particles...not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer...

  13. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... common E. coli histidine kinase. This hybrid construct senses light, and an obligate chromophore allows...intensity. In addition to these improvements, the constructs have been greatly streamlined; only two plasmids...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights You may also like....introns Anthony Leung Return to Top Expression Constructs Luciferase driven by a strong constitutive promoter...luciferase). Feng Zhang Return to Top Reporter Constructs Already know what gene or regulatory element ...reporter for? Browse or use the search box to find a construct that contains your regulatory element of interest...rapamycin-inducible interaction. nLuc and cLuc : Constructs for split firefly luciferase complementation ...and Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag...mScarlet-I. Find more NanoLuc® tools, including constructs for NanoBRET and NanoBiT assays, in the Promega...

  15. Worm Expression Resources

    Type
    Collection
    ...Worm Plasmids and Resources Plasmid Tools Worm Constructs Kits and Collections Other Resources The nematode...characterized which allow researchers to express desired constructs in precise cell types and/or developmental timeframes...plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples of useful...elegans . Synthetic Biology Synthetic biology constructs - Browse Addgene's collection of synthetic biology...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...experiments. Manufacturers rarely provide the construction plans or CAD (computer aided design) drawings... that allow for on-going creativity with the construction plans. Appropriate open-source licenses for ...

  17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...cells.  Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve ...cells are then transfected with several different constructs for the reporter to work and to make the desired...

  18. Hot Plasmids - February 2022

    Type
    Blog Post
    ... Figure 1: (a) Anti-FLAG frankenbody (FB) construction from the complementarity determining region ...and HA tags, you may not even need to clone new constructs to label your favorite protein! While the target...
Showing: 81 - 100 of 310 results