Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 168 results for: crispr library

Showing: 81 - 100 of 168 results

  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... (mTKO) CRISPR Library 159392 159393 Knockout Mouse Moffat 3rd ~5 19,463 mTF-CRISPRai Library 122131 Activation...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...within a given CRISPR library are unique to the genome of particular organism, and the library is only compatible...about each library can be found on the individual library page. Find Pooled Libraries of Interest...

  2. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the high-resolution tracking...Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function...

  3. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like to knockout a gene,...Service, we’re distributing lentivirus (with many CRISPR tools included among the preps that are currently...lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral... in experiments other than just those involving CRISPR and so we also have lentivirus for delivering control...

  4. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this... Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several shout-outs...shout-outs for making popular screening libraries like Brie and Brunello available to the scientific community...Use Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...

  5. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... TALEN kit to CRISPR/Cas9? When would you choose one or the other? Musunuru: The CRISPR/Cas9 system is...system for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be ... now makes possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with...15 base pairs is take four plasmids out of the library in the appropriate combination and do a restriction...be more efficient. You might often start with CRISPR/Cas9, but TALENs do have more flexibility in terms...data to date suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN ...

  6. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ....02364-19 More CRISPR Blog Posts from Addgene: CRISPR 101: Homology Directed Repair CRISPR Protocol for...Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale ... UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering is the fact that...NHEJ is consequently the principal means by which CRISPR/Cas9-introduced breaks are repaired. The following...variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs... Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell...S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted Nonhomologous End-Joining Strategy for...

  7. Lentiviral Prep Service

    Type
    Collection
    ...Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved...pooled libraries 92385 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition...Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B Human CRISPR inhibition library in backbone...In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses...knockout pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone...Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114...Root Human activation pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...of the special cases below:   Pooled Library A pooled library is a set of plasmids all built with the... Pooled libraries are normally supplied as liquid DNA with all of the plasmids in the library in a single...plasmids in a pooled library can range from a few hundred to millions. Pooled libraries must be verified ...sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs...help center article, "Can I deposit a pooled DNA library with Addgene?" or contact us at [email protected]...

  9. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...other CRISPR plasmids are from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled...pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the list ...years - the majority of these being lentiviral or CRISPR tools. How many times have these plasmids been ...lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from...come.  Resources on the Addgene blog Browse our CRISPR posts Read our viral vector blog posts Read our...guide Resources on the Addgene website Browse our CRISPR collection Find plasmids for your research  Learn...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...about CRISPR Pooled Library Experiments Additional resources on the Addgene website Find CRISPR Plasmids...on your CRISPR background with our CRISPR Guide Find Software Tools for gRNA Design    ...updated in 2022 by Susanna Stroik.  The advent of CRISPR/Cas9 has made it easier than ever to make precise... be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits Is the location...design programs. Find Validated gRNAs for Your Next CRISPR Experiment Cas9 expression systems Addgene carries...the various delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery ...genome edit can be found in our blog post entitled “CRISPR 101: Validating Your Genome Edit”. Is Cas9 not ...

  11. Resolute Plasmid Collection

    Type
    Collection
    ...Insert Industry PI Pooled Libraries CRISPR and overexpression pooled libraries are useful tools for genetic... Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...proteins. Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins...proteins. Mouse SLC Knockout Library 141436 Superti-Furga CRISPR knockout library targeting mouse SLC proteins...Transporter Knockout Libraries 213695 221409 221410 Superti-Furga CRISPR knockout library targeting human ...experiments. These libraries target the solute carrier family of proteins. Library ID PI Description Human... Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...

  12. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources...Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids...While much of CRISPR research has focused on genome editing, numerous discoveries have been made using...genomic region of interest targeted by a gRNA. CRISPR-mediated purification of a specific genomic region...method to purify a specific genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA,...require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification of a specific genomic region...Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic region...

  13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...exist in the CGC library but that would be useful to our model  so that we can use CRISPR/cas9 to build ...accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to modify the worm... crispr...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for...to GeCKO Library SAM Library Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...

  15. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Publication Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR Parasites CRISPR Plasmids...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and...

  16. CRISPR References and Information

    Type
    Collection
    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips... Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community...

  17. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...resources: plasmid, pooled library, blog posts, protocols, and more. Selecting crispr in Guides will return...for “CRISPR” plasmids keeps more than half of these. Instead we can navigate first to the CRISPR Resources... search field front and center. Let’s type in “crispr” and see what comes up. Before even clicking the...return links including our CRISPR guide.  For a closer look at this new functionality and ways to navigate...Type” you can filter by experimental purpose (e.g. CRISPR) Beyond search What can you do if you don’t have...Resources page: The page links to guides, our CRISPR eBook, plasmid kits, and curated pages categorized ...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Muscular Dystrophy with CRISPR Gene Editing Learn to Use CRISPR Pooled Libraries for Genome-Wide Screens...became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM to "shock and kill" To “shock ...HIV-1 out of the genome with CRISPR/Cas9 Before the introduction of CRISPR, HIV-1 genome editing with ...updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ... to vanquish these hidden viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel...University has demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate...genome from infected cells. Read on to learn how CRISPR can take on HIV-1 in vitro, and what obstacles ...

  19. CRISPR Plasmids and Resources

    Type
    Collection
    ...used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout, activation...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... information you need to use CRISPR in your own lab. Check out our CRISPR resources or browse plasmids...

  20. CRISPR Guide

    Type
    Collection
    ...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...Non-mammalian CRISPR libraries are also available, such as those for yeast or drosophila . Although CRISPR has ...
Showing: 81 - 100 of 168 results