We narrowed to 210 results for: cycs

1. What's New in CRISPR - Winter 2018

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   Blog Post

   ...transcription factors with GFP. As part of the Encyclopedia of DNA Elements (ENCODE), a consortium aimed...

2. Inntags: Innovative Protein Epitope Tagging

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   ...Georgieva et al. created tagged versions of the cyclin Cdc28 and the chaperone Ydj1, both of which display...

3. Six Spooky Science Stories and Halloween at Addgene

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   ...which get eaten by another snail, completing its lifecycle. Halloween genes The Halloween genes are a set...

4. Science as Art

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   ...on black screens, but not to know even the life cycle of the creature they deal with.” So photography ...

5. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   ...libraries include enrichment against kinases, cell cycle proteins, and more.  “In the past, genome engineering...

6. Bricking Science: Portraying Scientific Reality Through LEGO

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   ...work, see how many pictures we take to make a walk cycle of one step. Challenges in the LEGO science video...

7. Sleeping Beauty Awakens for Genome Engineering

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   ...gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase is proving an...

8. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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   ... Because Arabidopsis is small, has a short life cycle, and is relatively easy to grow, it is an ideal ...

9. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   ...limits its use for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists ...

10. Plasmids 101: Origin of Replication

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    ...ColE1 A Relaxed pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent...

11. Meet the New Addgene Board Members!

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    ...MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell and Paul Nurse,” Stegmeier...

12. Hot Plasmids and Viral Preps - September 2021

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    Blog Post

    ...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...

13. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ... with a freezer wired to a charger and two deep cycle batteries for power so the samples could be kept...

14. Plasmids 101: TOPO Cloning

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    ... to include extra extension time after the last cycle of PCR to make sure that the "A" gets added to all...

15. New and Upcoming Viral Vectors - June 2019

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    ...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

16. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ...viruses rely on the help of human proteins in their lifecycle, identifying these potential human helpers of ...

17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ... blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in Research...

18. A Quick Guide to a Career in Software Product Management

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    ... the users, gather more feedback, and start the cycle over again to refine the solution, or to come up...

19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post

    ...Cas9 expression is activated using 4-OH-T and doxycycline. A gRNA that targets your desired gene (whose...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...