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We narrowed to 100 results for: dcas9, grna

Showing: 81 - 100 of 100 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...coated beads. Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the... Cas13, upon finding complementarity between the gRNA and a given fluorescently labelled substrate, gets... E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli harboring the defective...expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers...

  3. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching...Base editors use either catalytically dead Cas9 (dCas9) or a Cas9 nickase (nCas9) to avoid introducing ... the DNA strand that does not base pair with the gRNA. Base editors target this “non-target strand.” To... predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:...

  6. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is ...PEmax to enhance pegRNA stability epegRNA - additional protection added to 3’ tail of pegRNA to prevent RNA...nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI ...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...1143-8.  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets...DNA break. Next, the gRNA being tested is expressed along with Cas9. If the gRNA effectively cuts the ...for scientists to validate the efficacy of their gRNAs. The first step is to clone the target sequence ...CRISPRS. Developed by David Liu and colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  8. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL...containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The...Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor CEBPB Human FLAG...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...#79888) , which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid...

  9. Modular Cloning Guide

    Type
    Guide
    ...expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use...plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome editing in monocots...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...of applications, including CRISPR activation by dCas9-VPR, prime editing, and delivery of disease-relevant...engineered pegRNAs (epegRNAs), which add a structural motif to stabilize the 3’ end of the pegRNA. As research...The team designed sgRNAs that include an internal barcode and separated multiple sgRNAs with tRNAs, which...BFP2.     Figure 1: CROPseq-multi uses two sgRNAs with internal barcodes (iBARs), multiplexed using...lentiviral integration, producing a second copy of the sgRNAs. Image reused from Walton et al. 2024 under a CC-BY-NC-ND...occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the lentiviral minus strand in ...of host cell proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi ...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries ...Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful...experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic ...thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions... Gateway compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors...Plasmids and Resources collection, and browse our Empty gRNA Expression Vectors page Cre-lox Site-specific recombination...selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral...- Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral...Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops...Lentiviral expression plasmid for two sgRNAs from an hU6 and an mU6 promoter Protein-protein interaction...

  14. Validated gRNA Sequences

    Type
    Collection
    ... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA Target...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences that have...Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...details and outcomes. Submit Validated gRNA Sequences Validated gRNA sequences can be added to this table...validated gRNA sequences. Genome...

  15. Zhang Lab CRISPR Page

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from the Zhang lab...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids... for transient transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the...production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse...CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven...

  16. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...lab. The Bikard library contains ~93,000 unique gRNAs that target random positions along the E. coli MG1655...targeting requires two sgRNAS: one that targets the knock-in site and a universal sgRNA that targets the donor... the genomic loci of interest. As the universal sgRNA is not predicted to target sites in the human, pig.... The lab has previously used the library with SpdCas9 to perform pooled CRISPRi screens to identify essential...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76361 TBK1 gRNA (BRDN0001145663) TBK1...David Root 76362 TBK1 gRNA (BRDN0001148392) TBK1 U6 ALS David Root 76363 TBK1 gRNA (BRDN0001148607) TBK1...David Root 76470 GAK gRNA (BRDN0001146687) GAK U6 Parkinson's David Root 76471 GAK gRNA (BRDN0001146263) ...David Root 76472 GAK gRNA (BRDN0001148048) GAK U6 Parkinson's David Root 76809 PANK2 gRNA (BRDN0001149459)...
Showing: 81 - 100 of 100 results