Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 435 results for: erg

Showing: 81 - 100 of 435 results

  1. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...sequences. Repeating sequences within a plasmid can undergo intramolecular DNA recombination. This naturally...two repeating elements love each other very much undergo recombination, two smaller plasmids are formed....To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers find that...

  2. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... An undergraduate can mentor a high school student, a graduate student can mentor an undergraduate, a ... up on plans. Make a commitment to put time and energy into your mentoring relationships. You will only...

  3. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...non-governmental groups with seeking “public views on emerging IVF techniques designed to prevent the transmission... a controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR/Cas9 –...in the software industry to inform her work on emerging issues in novel technologies, including self-driving...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Instead, R body monomers respond to pH changes by undergoing local conformational changes. These tiny movements...decades, and they were known to be resistant to detergents and degradation under a variety of conditions...conditions. More speculatively, R bodies might endow divergent organisms with new functions. For inspiration,...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...ll provide information about commonly used and emerging capsids to help you decide which ones may be suitable... of published descriptions of commonly used or emerging capsids. Selected capsids are further highlighted... regional bias towards the cortex and thalamus emerges. AAV-PHP.eC effectively transduces neurons and ... Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg, M. C., Lagor, W. R., Heaney, J. D., Chan, Y. ... Sakopoulos, S., Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith...

  6. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered...distinct effector domains that work together synergistically, modeled after natural transcription activation... J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015...

  7. Tags and Other Markers

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...

  8. Neuroscience

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...

  9. TREAT-AD Plasmid Collection

    Type
    Collection
    ...disease (AD) by generating validated resources for emerging targets in AD and creating high-quality, well-...

  11. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...cargoes, genetically engineered neurotropic viruses emerged as powerful tools for visualizing neural connectivity...monosynaptic tracing to identify inputs to two major dopaminergic cell populations in two specific brain regions...617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, Lepier A, Götz M, Berninger...

  13. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...obstacles when working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition...repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication. To avoid...immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane...

  14. Antibodies

    Type
    Collection
    ... are expressed from plasmids. These antibodies undergo application-specific validation and consistent ...

  15. KLF Research Plasmids

    Type
    Collection
    ...Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About ...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t...accurately suggests the potential of this protein to undergo conformational shifts. This backbone model of Cas9...conformational gymnastics that this protein must undergo as it interrogates your genome. If you are interested...

  17. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...takes roughly 10 days for an egg to develop and emerge as an adult fly at 25°C. At low temperatures like...the day and throughout daytime. They mostly stop emerging at night. An incubator with a light cycle makes...makes for very predictable work! When flies first emerge, their bodies will be very light in coloration ...

  18. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...I knew that when I was “managing” a couple of undergrads back in grad school. Both were hard-working scientists-in-training...reviewing the results of my Myers-Briggs test:  my undergrads were different people! More specifically, they...time I could likely have retained this talented undergrad. The point here is managing is not “one size ...

  19. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... media, while others remain cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo...rAAV sample has to be digested with DNase before undergoing titering, to remove residual plasmid DNA carried...Article 10. https://doi.org/10.1089/hum.2009.223 Vandenberghe, L. H., Xiao, R., Lock, M., Lin, J., Korn, M...
Showing: 81 - 100 of 435 results